Restriction map for
pF1KB8582
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AocI Aor51HI AscI AseI
AsnI Asp718I AvrII BamHI BbrPI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsiWI BspDI
BspHI BspLU11I BsrDI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI Eco47III
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI HpaI KpnI KspI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspI StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 130 CGCG CG^CG
AccIII 147 TCCGGA T^CCGGA
AhdI 73 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1225 GTGCAC G^TGCAC
ApaI 1266 GGGCCC GGGCC^C
ApaLI 1225 GTGCAC G^TGCAC
Asp700I 1662 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 73 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 400 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1163 TGCGCA TGC^GCA
BbeI 26 GGCGCC GGCGC^C
BcgI 1363 CGAATGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2054 GGATAC GTATCC(6/5)
BfaI 58 CTAG C^TAG
BsaAI 1014 CACGTA [CT]AC^GT[GA]
BsaWI 147 TCCGGA [AT]^CCGG[AT]
BseAI 147 TCCGGA T^CCGGA
BsiEI 812 CGGCCG CG[GA][CT]^CG
BspEI 147 TCCGGA T^CCGGA
BstEII 621 GGTTACC G^GT[ACGT]ACC
BstPI 621 GGTTACC G^GT[ACGT]ACC
BstUI 130 CGCG CG^CG
BstXI 770 CCATACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1990 TTTAAA TTT^AAA
DsaI 455 CCATGG C^C[GA][CT]GG
EagI 812 CGGCCG C^GGCCG
Eam1105I 73 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 812 CGGCCG C^GGCCG
Eco52I 812 CGGCCG C^GGCCG
EcoO65I 621 GGTTACC G^GT[ACGT]ACC
EcoRI 432 GAATTC G^AATTC
FspI 1163 TGCGCA TGC^GCA
HaeII 26 GGCGCC [GA]GCGC^[CT]
HindIII 733 AAGCTT A^AGCTT
KasI 26 GGCGCC G^GCGCC
MaeI 58 CTAG C^TAG
MaeII 1015 ACGT A^CGT
MroI 147 TCCGGA T^CCGGA
MseI 1991 TTAA T^TAA
MvnI 130 CGCG CG^CG
NarI 26 GGCGCC GG^CGCC
NcoI 455 CCATGG C^CATGG
NspI 875 GCATGC [GA]CATG^[CT]
PflFI 400 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1250 GGGTCCT [GA]G^G[AT]CC[CT]
SalI 1456 GTCGAC G^TCGAC
SfoI 26 GGCGCC GGC^GCC
SmaI 1823 CCCGGG CCC^GGG
SphI 875 GCATGC GCATG^C
TaqI 1457 TCGA T^CGA
Tru9I 1991 TTAA T^TAA
Tth111I 400 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1457 TCGA T^CGA
XcmI 1308 CCAAGTCATCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1823 CCCGGG C^CCGGG
XmaI 1823 CCCGGG C^CCGGG
XmnI 1662 GAAGTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1294 GTCTAC GT^[AC][GT]AC
1456 GTCGAC
AcyI 26 GGCGCC G[GA]^CG[CT]C
1780 GGCGTC
BalI 542 TGGCCA TGG^CCA
1688 TGGCCA
BbsI 1921 GAAGAC GAAGAC(2/6)
2089 GAAGAC
BbvCI 948 CCTCAGC CCTCAGC(-5/-2)
571 GCTGAGG
BpuAI 1921 GAAGAC GAAGAC(2/6)
2089 GAAGAC
BsaHI 26 GGCGCC G[GA]^CG[CT]C
1780 GGCGTC
BsgI 1600 CTGCAC GTGCAG(16/14)
1708 GTGCAG
BsmBI 1782 CGTCTC CGTCTC(1/5)
796 GAGACG
BsmI 1364 GAATGC GAATGC(1/-1)
607 GCATTC
Bsp1407I 1007 TGTACA T^GTACA
1208 TGTACA
BsrGI 1007 TGTACA T^GTACA
1208 TGTACA
BstYI 1886 AGATCC [GA]^GATC[CT]
2223 GGATCT
Hin1I 26 GGCGCC G[GA]^CG[CT]C
1780 GGCGTC
HincII 1456 GTCGAC GT[CT]^[GA]AC
1531 GTCAAC
HindII 1456 GTCGAC GT[CT]^[GA]AC
1531 GTCAAC
HphI 1037 TCACC GGTGA(8/7)
1586 TCACC
MflI 1886 AGATCC [GA]^GATC[CT]
2223 GGATCT
MluNI 542 TGGCCA TGG^CCA
1688 TGGCCA
MscI 542 TGGCCA TGG^CCA
1688 TGGCCA
PflMI 510 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1284 CCAGCCCTTGG
SacI 1401 GAGCTC GAGCT^C
1485 GAGCTC
SbfI 258 CCTGCAGG CCTGCA^GG
1536 CCTGCAGG
SmlI 883 CTCAAG C^T[CT][GA]AG
997 CTCAAG
Sse8387I 258 CCTGCAGG CCTGCA^GG
1536 CCTGCAGG
SspBI 1007 TGTACA T^GTACA
1208 TGTACA
TfiI 85 GAATC G^A[AT]TC
2039 GATTC
Van91I 510 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1284 CCAGCCCTTGG
XhoII 1886 AGATCC [GA]^GATC[CT]
2223 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
Tsp509I TspRI