Restriction map for
pF1KB8588
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AcyI AflIII AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AvaI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BglI BglII BlnI BlpI Bpu1102I
BsaHI BsaI BsgI BsiEI BsiWI
BsmBI BsoBI BspLU11I BspMI BsrDI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII CfoI Cfr10I
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoT22I FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PstI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 1714 GTAGAC GT^[AC][GT]AC
AccII 3220 CGCG CG^CG
AccIII 2486 TCCGGA T^CCGGA
AclI 2438 AACGTT AA^CGTT
Alw44I 2350 GTGCAC G^TGCAC
AlwNI 1721 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3382 CCTGAGG CC^T[ACGT]AGG
ApaLI 2350 GTGCAC G^TGCAC
AseI 2329 ATTAAT AT^TAAT
AsnI 2329 ATTAAT AT^TAAT
Asp700I 1282 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1024 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1953 TGGCCA TGG^CCA
BanI 396 GGTGCC G^G[CT][GA]CC
BpmI 3410 CTCCAG CTGGAG(16/14)
BsaAI 1591 TACGTA [CT]AC^GT[GA]
BseAI 2486 TCCGGA T^CCGGA
BseRI 3416 CTCCTC GAGGAG(10/8)
BsmI 2707 GCATTC GAATGC(1/-1)
Bsp1407I 2290 TGTACA T^GTACA
BspDI 3194 ATCGAT AT^CGAT
BspEI 2486 TCCGGA T^CCGGA
BsrGI 2290 TGTACA T^GTACA
BssSI 279 CACGAG CACGAG(-5/-1)
BstUI 3220 CGCG CG^CG
BstXI 1398 CCAAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2481 GGATCT [GA]^GATC[CT]
Bsu36I 3382 CCTGAGG CC^T[ACGT]AGG
ClaI 3194 ATCGAT AT^CGAT
DraI 1228 TTTAAA TTT^AAA
EaeI 1953 TGGCCA [CT]^GGCC[GA]
Eco81I 3382 CCTGAGG CC^T[ACGT]AGG
EcoRI 2399 GAATTC G^AATTC
EcoRV 1898 GATATC GAT^ATC
EcoT14I 3519 CCAAGG C^C[AT][AT]GG
HindIII 1438 AAGCTT A^AGCTT
MflI 2481 GGATCT [GA]^GATC[CT]
MluNI 1953 TGGCCA TGG^CCA
MroI 2486 TCCGGA T^CCGGA
MscI 1953 TGGCCA TGG^CCA
MvnI 3220 CGCG CG^CG
PflFI 1024 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1061 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2329 ATTAAT AT^TAAT
Psp1406I 2438 AACGTT AA^CGTT
SfcI 5 CTACAG C^T[GA][CT]AG
SnaBI 1591 TACGTA TAC^GTA
SphI 875 GCATGC GCATG^C
SspBI 2290 TGTACA T^GTACA
SspI 1866 AATATT AAT^ATT
StyI 3519 CCAAGG C^C[AT][AT]GG
Tth111I 1024 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 2481 GGATCT [GA]^GATC[CT]
XmnI 1282 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 276 GAGCAC G[AT]GC[AT]^C
2350 GTGCAC
AvaII 2208 GGTCC G^G[AT]CC
3516 GGACC
BciVI 3534 GGATAC GTATCC(6/5)
1787 GTATCC
BclI 495 TGATCA T^GATCA
1995 TGATCA
BsaBI 823 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1894 GATGGATATC
BsaWI 426 ACCGGA [AT]^CCGG[AT]
2486 TCCGGA
BsiHKAI 276 GAGCAC G[AT]GC[AT]^C
2350 GTGCAC
BspHI 969 TCATGA T^CATGA
1937 TCATGA
BsrBI 1641 CCGCTC CCGCTC(-3/-3)
3068 CCGCTC
BstAPI 1876 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2707 GCATTCTGTGC
Cfr13I 2208 GGTCC G^G[ACGT]CC
3516 GGACC
FbaI 495 TGATCA T^GATCA
1995 TGATCA
HaeIII 1157 GGCC GG^CC
1954 GGCC
MamI 823 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1894 GATGGATATC
MspA1I 8 CAGCTG C[AC]G^C[GT]G
2120 CAGCTG
NdeI 1220 CATATG CA^TATG
2272 CATATG
NspI 875 GCATGC [GA]CATG^[CT]
3059 ACATGC
PflMI 2033 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2812 CCATTACCTGG
PvuII 8 CAGCTG CAG^CTG
2120 CAGCTG
RcaI 969 TCATGA T^CATGA
1937 TCATGA
SapI 273 GAAGAGC GCTCTTC(1/4)
1654 GCTCTTC
Sau96I 2208 GGTCC G^G[ACGT]CC
3516 GGACC
ScaI 2464 AGTACT AGT^ACT
2545 AGTACT
Van91I 2033 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2812 CCATTACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI BbsI BbvI BcnI
BfaI BfrI BmyI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI ScrFI SfaNI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I