Restriction map for
pF1KB8594
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# NO CUT ENZYMES ( 150 enzymes) #
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Acc65I AccII AccIII AciI AclI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvrII BalI
BamHI BanI BbeI BbrPI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FauI FbaI FseI HaeII HaeIII
HgaI HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 194 GACGTC GACGT^C
AccI 152 GTCGAC GT^[AC][GT]AC
AcyI 194 GACGTC G[GA]^CG[CT]C
AflIII 1228 ACATGT A^C[GA][CT]GT
AviII 899 TGCGCA TGC^GCA
BanII 524 GAGCTC G[GA]GC[CT]^C
BbvCI 421 GCTGAGG CCTCAGC(-5/-2)
BcgI 1113 GCAGATCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 286 GGATAC GTATCC(6/5)
BcnI 380 CCGGG CC^[GC]GG
BfaI 521 CTAG C^TAG
BglI 974 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 20 CTCCAG CTGGAG(16/14)
BsaHI 194 GACGTC G[GA]^CG[CT]C
BsmFI 655 GTCCC GGGAC(10/14)
BspHI 738 TCATGA T^CATGA
BspLU11I 1228 ACATGT A^CATGT
BsrDI 1091 GCAATG GCAATG(2/0)
BstNI 26 CCTGG CC^[AT]GG
DraII 62 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoNI 199 CCTAATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 62 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1261 GAATTC G^AATTC
EcoRII 26 CCTGG ^CC[AT]GG
FspI 899 TGCGCA TGC^GCA
HapII 380 CCGG C^CGG
Hin1I 194 GACGTC G[GA]^CG[CT]C
HpaII 380 CCGG C^CGG
HphI 1032 TCACC GGTGA(8/7)
MaeI 521 CTAG C^TAG
MaeII 195 ACGT A^CGT
MspI 380 CCGG C^CGG
MvaI 26 CCTGG CC^[AT]GG
MwoI 974 GCCGTGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 380 CCGGG CC^[GC]GG
NcoI 291 CCATGG C^CATGG
NspI 1228 ACATGT [GA]CATG^[CT]
PpuMI 62 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 485 CTGCAG CTGCA^G
RcaI 738 TCATGA T^CATGA
SacI 524 GAGCTC GAGCT^C
SalI 152 GTCGAC G^TCGAC
ScaI 1027 AGTACT AGT^ACT
SfaNI 534 GATGC GCATC(5/9)
SfcI 485 CTGCAG C^T[GA][CT]AG
XbaI 520 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AspHI 524 GAGCTC G[AT]GC[AT]^C
1232 GTGCTC
BmyI 524 GAGCTC G[AGT]GC[ACT]^C
1232 GTGCTC
BsiHKAI 524 GAGCTC G[AT]GC[AT]^C
1232 GTGCTC
BsmAI 941 GAGAC GTCTC(1/5)
1402 GAGAC
BsmI 896 GAATGC GAATGC(1/-1)
105 GCATTC
Bsp1286I 524 GAGCTC G[AGT]GC[ACT]^C
1232 GTGCTC
BspMI 663 ACCTGC ACCTGC(4/8)
487 GCAGGT
BssKI 26 CCTGG ^CC[ACGT]GG
380 CCGGG
Cac8I 979 GCTGGC GC[ACGT]^[ACGT]GC
1199 GCTGGC
CfoI 4 GCGC GCG^C
900 GCGC
DpnII 1116 GATC ^GATC
1332 GATC
DraI 438 TTTAAA TTT^AAA
775 TTTAAA
DsaI 291 CCATGG C^C[GA][CT]GG
1354 CCGTGG
HhaI 4 GCGC GCG^C
900 GCGC
HinP1I 4 GCGC G^CGC
900 GCGC
HincII 152 GTCGAC GT[CT]^[GA]AC
853 GTTGAC
HindII 152 GTCGAC GT[CT]^[GA]AC
853 GTTGAC
MboI 1116 GATC ^GATC
1332 GATC
MslI 277 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
961 CATGAAGATG
NdeII 1116 GATC ^GATC
1332 GATC
PleI 1000 GACTC GAGTC(4/5)
742 GAGTC
Sau3AI 1116 GATC ^GATC
1332 GATC
ScrFI 26 CCTGG CC^[ACGT]GG
380 CCGGG
SmlI 1079 CTCAAG C^T[CT][GA]AG
1165 CTTGAG
Tsp45I 606 GTCAC ^GT[GC]AC
1337 GTGAC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbsI BbvI BpuAI BsaJI BsiYI
BslI BsrI BstF5I Cfr13I DdeI
EarI EcoT14I Fnu4HI FokI HinfI
ItaI Ksp632I MaeIII MboII MnlI
MseI NlaIII RsaI Sau96I StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I