Restriction map for
pF1KB8603
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccII AciI AclI
AcyI AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BalI BbeI BbrPI BcgI BciVI
BclI BglI BglII BlnI BpmI
BsaAI BsaBI BsaHI BsaI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT14I FauI FbaI FseI
FspI Hin1I HindIII HpaI HphI
KasI KspI MamI MluI MluNI
MscI MspA1I MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 110 GGTACC G^GTACC
AccIII 671 TCCGGA T^CCGGA
AflII 289 CTTAAG C^TTAAG
AlwNI 1184 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1112 AGCGCT AGC^GCT
Asp718I 110 GGTACC G^GTACC
AspHI 939 GAGCAC G[AT]GC[AT]^C
AvaI 932 CTCGAG C^[CT]CG[GA]G
AvaII 354 GGACC G^G[AT]CC
BamHI 550 GGATCC G^GATCC
BanII 922 GGGCTC G[GA]GC[CT]^C
BbsI 871 GAAGAC GAAGAC(2/6)
BbvCI 713 CCTCAGC CCTCAGC(-5/-2)
BcnI 427 CCCGG CC^[GC]GG
BfaI 572 CTAG C^TAG
BfrI 289 CTTAAG C^TTAAG
BlpI 309 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 309 GCTGAGC GC^T[ACGT]AGC
BpuAI 871 GAAGAC GAAGAC(2/6)
BsaJI 167 CCAGGG C^C[ACGT][ACGT]GG
BsaWI 671 TCCGGA [AT]^CCGG[AT]
BseAI 671 TCCGGA T^CCGGA
BsgI 191 GTGCAG GTGCAG(16/14)
BsiHKAI 939 GAGCAC G[AT]GC[AT]^C
BsoBI 932 CTCGAG C^[CT]CG[GA]G
BspEI 671 TCCGGA T^CCGGA
BspMI 122 ACCTGC ACCTGC(4/8)
BstAPI 376 GCATCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 838 GCCTGC GC[ACGT]^[ACGT]GC
CelII 309 GCTGAGC GC^T[ACGT]AGC
Eco47III 1112 AGCGCT AGC^GCT
EcoRI 196 GAATTC G^AATTC
EcoRV 784 GATATC GAT^ATC
EcoT22I 374 ATGCAT ATGCA^T
HaeII 1112 AGCGCT [GA]GCGC^[CT]
HgaI 241 GACGC GACGC(5/10)
HincII 1060 GTTGAC GT[CT]^[GA]AC
HindII 1060 GTTGAC GT[CT]^[GA]AC
KpnI 110 GGTACC GGTAC^C
MaeI 572 CTAG C^TAG
MfeI 1164 CAATTG C^AATTG
MroI 671 TCCGGA T^CCGGA
MslI 471 CATTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1164 CAATTG C^AATTG
NciI 427 CCCGG CC^[GC]GG
NsiI 374 ATGCAT ATGCA^T
PaeR7I 932 CTCGAG C^TCGAG
PleI 950 GACTC GAGTC(4/5)
PstI 257 CTGCAG CTGCA^G
SexAI 1049 ACCTGGT A^CC[AT]GGT
XhoI 932 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 4 GAATTT [GA]^AATT[CT]
196 GAATTC
AfaI 111 GTAC GT^AC
1217 GTAC
ApoI 4 GAATTT [GA]^AATT[CT]
196 GAATTC
Asp700I 418 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
679 GAACTGATTC
BanI 110 GGTACC G^G[CT][GA]CC
975 GGTGCC
BseRI 748 GAGGAG GAGGAG(10/8)
857 GAGGAG
BsiYI 421 CCTGTTCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1220 CCCACTTGAGG
BslI 421 CCTGTTCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1220 CCCACTTGAGG
BsmFI 732 GGGAC GGGAC(10/14)
948 GGGAC
BsmI 846 GAATGC GAATGC(1/-1)
65 GCATTC
BspHI 911 TCATGA T^CATGA
1175 TCATGA
Cfr13I 354 GGACC G^G[ACGT]CC
1075 GGGCC
EarI 502 CTCTTC CTCTTC(1/4)
532 GAAGAG
HaeIII 403 GGCC GG^CC
1076 GGCC
HapII 428 CCGG C^CGG
672 CCGG
HpaII 428 CCGG C^CGG
672 CCGG
Ksp632I 502 CTCTTC CTCTTC(1/4)
532 GAAGAG
MaeII 332 ACGT A^CGT
579 ACGT
MspI 428 CCGG C^CGG
672 CCGG
RcaI 911 TCATGA T^CATGA
1175 TCATGA
RsaI 111 GTAC GT^AC
1217 GTAC
SapI 532 GAAGAGC GCTCTTC(1/4)
501 GCTCTTC
Sau96I 354 GGACC G^G[ACGT]CC
1075 GGGCC
TaqI 855 TCGA T^CGA
933 TCGA
TfiI 262 GATTC G^A[AT]TC
684 GATTC
TthHB8I 855 TCGA T^CGA
933 TCGA
XmnI 418 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
679 GAACTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AluI AlwI BbvI BmyI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI CfoI DdeI DpnII EcoRII
Fnu4HI FokI HhaI HinP1I HinfI
ItaI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI ScrFI SfaNI
SfcI SmlI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII