Restriction map for
pF1KB8615
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI Alw44I Aor51HI ApaLI ApoI
AscI Asp700I Asp718I AspI AvaII
AvrII BalI BamHI BbeI BbrPI
BbvCI BciVI BclI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DrdI EagI EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoT22I
FauI FbaI FseI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 505 CGCG CG^CG
AlwI 1075 GGATC GGATC(4/5)
AlwNI 350 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 268 CCTGAGG CC^T[ACGT]AGG
ApaI 277 GGGCCC GGGCC^C
AseI 600 ATTAAT AT^TAAT
AsnI 600 ATTAAT AT^TAAT
AviII 915 TGCGCA TGC^GCA
BanI 1246 GGTGCC G^G[CT][GA]CC
BcgI 1333 GCAGACACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 497 CTAG C^TAG
BseRI 775 GAGGAG GAGGAG(10/8)
BsgI 480 CTGCAC GTGCAG(16/14)
BsmFI 1441 GTCCC GGGAC(10/14)
BsmI 912 GAATGC GAATGC(1/-1)
BspHI 509 TCATGA T^CATGA
BsrFI 1056 GCCGGC [GA]^CCGG[CT]
BstAPI 1388 GCAATCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 505 CGCG CG^CG
BstXI 698 CCAGTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 268 CCTGAGG CC^T[ACGT]AGG
CfoI 916 GCGC GCG^C
Cfr10I 1056 GCCGGC [GA]^CCGG[CT]
DraIII 425 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 635 CCATGG C^C[GA][CT]GG
Eco81I 268 CCTGAGG CC^T[ACGT]AGG
EcoNI 268 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 500 GATATC GAT^ATC
FspI 915 TGCGCA TGC^GCA
HgaI 506 GCGTC GACGC(5/10)
HhaI 916 GCGC GCG^C
HinP1I 916 GCGC G^CGC
MaeI 497 CTAG C^TAG
MaeII 1453 ACGT A^CGT
MslI 19 CATGTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 505 CGCG CG^CG
NaeI 1056 GCCGGC GCC^GGC
NcoI 635 CCATGG C^CATGG
NgoMIV 1056 GCCGGC G^CCGGC
PleI 683 GACTC GAGTC(4/5)
PshBI 600 ATTAAT AT^TAAT
RcaI 509 TCATGA T^CATGA
SacI 778 GAGCTC GAGCT^C
SspI 448 AATATT AAT^ATT
StuI 265 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 337 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
648 GACTGCTCGTC
AspEI 337 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
648 GACTGCTCGTC
AspHI 94 GTGCTC G[AT]GC[AT]^C
778 GAGCTC
AvaI 781 CTCGGG C^[CT]CG[GA]G
1285 CCCGAG
BanII 277 GGGCCC G[GA]GC[CT]^C
778 GAGCTC
BbsI 1205 GAAGAC GAAGAC(2/6)
1278 GAAGAC
BglII 38 AGATCT A^GATCT
395 AGATCT
BpuAI 1205 GAAGAC GAAGAC(2/6)
1278 GAAGAC
BsiHKAI 94 GTGCTC G[AT]GC[AT]^C
778 GAGCTC
BsmAI 681 GAGAC GTCTC(1/5)
1019 GTCTC
BsoBI 781 CTCGGG C^[CT]CG[GA]G
1285 CCCGAG
BsrI 1173 ACTGG ACTGG(1/-1)
698 CCAGT
BssSI 1401 CACGAG CACGAG(-5/-1)
383 CTCGTG
BstNI 90 CCTGG CC^[AT]GG
1349 CCAGG
EaeI 722 TGGCCG [CT]^GGCC[GA]
1058 CGGCCA
Eam1105I 337 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
648 GACTGCTCGTC
EcoRII 90 CCTGG ^CC[AT]GG
1349 CCAGG
EcoT14I 635 CCATGG C^C[AT][AT]GG
1268 CCTTGG
HphI 1011 GGTGA GGTGA(8/7)
1044 TCACC
MspA1I 718 CAGCTG C[AC]G^C[GT]G
817 CAGCTG
MvaI 90 CCTGG CC^[AT]GG
1349 CCAGG
PvuII 718 CAGCTG CAG^CTG
817 CAGCTG
SmlI 406 CTTGAG C^T[CT][GA]AG
876 CTCAAG
StyI 635 CCATGG C^C[AT][AT]GG
1268 CCTTGG
TaqI 400 TCGA T^CGA
812 TCGA
TfiI 303 GATTC G^A[AT]TC
352 GATTC
TthHB8I 400 TCGA T^CGA
812 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeIII MboI MboII MflI MnlI
MseI MspI MwoI NciI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII