Restriction map for
pF1KB8627
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BssHII BstEII BstPI
BstUI Bsu36I CelII ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Van91I
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1637 GTATAC GT^[AC][GT]AC
AclI 594 AACGTT AA^CGTT
AflII 1138 CTTAAG C^TTAAG
Aor51HI 2299 AGCGCT AGC^GCT
Asp700I 1237 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1079 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1670 TGGCCA TGG^CCA
BbsI 356 GTCTTC GAAGAC(2/6)
BbvCI 85 GCTGAGG CCTCAGC(-5/-2)
BfrI 1138 CTTAAG C^TTAAG
BpuAI 356 GTCTTC GAAGAC(2/6)
BsgI 1720 GTGCAG GTGCAG(16/14)
BsmFI 19 GGGAC GGGAC(10/14)
Bsp1407I 876 TGTACA T^GTACA
BspHI 1086 TCATGA T^CATGA
BsrFI 100 GCCGGT [GA]^CCGG[CT]
BsrGI 876 TGTACA T^GTACA
BssSI 1762 CTCGTG CACGAG(-5/-1)
Bst1107I 1637 GTATAC GTA^TAC
BstBI 359 TTCGAA TT^CGAA
BstXI 461 CCAAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1637 GTATAC GTA^TAC
Cfr10I 100 GCCGGT [GA]^CCGG[CT]
DraI 1573 TTTAAA TTT^AAA
EaeI 1670 TGGCCA [CT]^GGCC[GA]
EarI 1563 GAAGAG CTCTTC(1/4)
Eco47III 2299 AGCGCT AGC^GCT
EcoNI 1464 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1509 GATATC GAT^ATC
Ksp632I 1563 GAAGAG CTCTTC(1/4)
MluNI 1670 TGGCCA TGG^CCA
MscI 1670 TGGCCA TGG^CCA
NdeI 971 CATATG CA^TATG
NspI 2010 GCATGC [GA]CATG^[CT]
NspV 359 TTCGAA TT^CGAA
PaeR7I 112 CTCGAG C^TCGAG
PflFI 1079 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 670 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 594 AACGTT AA^CGTT
PstI 304 CTGCAG CTGCA^G
RcaI 1086 TCATGA T^CATGA
SacI 1738 GAGCTC GAGCT^C
ScaI 1239 AGTACT AGT^ACT
SfuI 359 TTCGAA TT^CGAA
SphI 2010 GCATGC GCATG^C
SspBI 876 TGTACA T^GTACA
Tth111I 1079 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1173 TCTAGA T^CTAGA
XhoI 112 CTCGAG C^TCGAG
XmnI 1237 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 379 AAATTC [GA]^AATT[CT]
1936 AAATTC
AlwNI 1319 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2122 CAGACTCTG
ApoI 379 AAATTC [GA]^AATT[CT]
1936 AAATTC
AvaII 20 GGACC G^G[AT]CC
2136 GGACC
BanI 254 GGTGCC G^G[CT][GA]CC
2027 GGCACC
BglI 1663 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1957 GCCCAGCAGGC
BglII 453 AGATCT A^GATCT
1896 AGATCT
BsaBI 937 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1488 GATGAGGATC
BseRI 115 GAGGAG GAGGAG(10/8)
1471 GAGGAG
BsmAI 27 GAGAC GTCTC(1/5)
1315 GAGAC
BsmI 1270 GAATGC GAATGC(1/-1)
367 GCATTC
BstAPI 1538 GCAAAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2207 GCACTCTCTGC
CfoI 2160 GCGC GCG^C
2300 GCGC
FauI 143 CCCGC CCCGC(4/6)
1856 CCCGC
HaeII 2159 GGCGCT [GA]GCGC^[CT]
2299 AGCGCT
HhaI 2160 GCGC GCG^C
2300 GCGC
HinP1I 2160 GCGC G^CGC
2300 GCGC
HindIII 1294 AAGCTT A^AGCTT
1578 AAGCTT
MaeII 287 ACGT A^CGT
595 ACGT
MamI 937 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1488 GATGAGGATC
SfcI 304 CTGCAG C^T[GA][CT]AG
662 CTATAG
SmaI 1310 CCCGGG CCC^GGG
2202 CCCGGG
TaqI 113 TCGA T^CGA
360 TCGA
TthHB8I 113 TCGA T^CGA
360 TCGA
XmaCI 1310 CCCGGG C^CCGGG
2202 CCCGGG
XmaI 1310 CCCGGG C^CCGGG
2202 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII