Restriction map for
pF1KB8629
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanII
BcgI BciVI BfrI BglI BglII
BlnI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsoBI BspEI BspHI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI Ksp632I
KspI MfeI MflI MluI MroI
MunI MvnI NaeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccI 1297 GTCGAC GT^[AC][GT]AC
AcyI 1028 GGCGCC G[GA]^CG[CT]C
AlwNI 1100 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1291 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 250 GGACC G^G[AT]CC
BalI 471 TGGCCA TGG^CCA
BbeI 1028 GGCGCC GGCGC^C
BbrPI 916 CACGTG CAC^GTG
BbvCI 86 CCTCAGC CCTCAGC(-5/-2)
BclI 377 TGATCA T^GATCA
BcnI 939 CCGGG CC^[GC]GG
BlpI 819 GCTAAGC GC^T[ACGT]AGC
BmyI 913 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 819 GCTAAGC GC^T[ACGT]AGC
BsaAI 916 CACGTG [CT]AC^GT[GA]
BsaBI 1230 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1028 GGCGCC G[GA]^CG[CT]C
BseRI 509 CTCCTC GAGGAG(10/8)
BsgI 981 CTGCAC GTGCAG(16/14)
BsmAI 1021 GTCTC GTCTC(1/5)
BsmBI 1020 CGTCTC CGTCTC(1/5)
BsmFI 328 GGGAC GGGAC(10/14)
BsmI 1410 GAATGC GAATGC(1/-1)
Bsp1286I 913 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 920 TGTACA T^GTACA
BspDI 1237 ATCGAT AT^CGAT
BsrGI 920 TGTACA T^GTACA
BssSI 1023 CTCGTG CACGAG(-5/-1)
BstBI 1121 TTCGAA TT^CGAA
CelII 819 GCTAAGC GC^T[ACGT]AGC
CfoI 1029 GCGC GCG^C
ClaI 1237 ATCGAT AT^CGAT
DraIII 691 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 578 CCATGG C^C[GA][CT]GG
EaeI 471 TGGCCA [CT]^GGCC[GA]
FbaI 377 TGATCA T^GATCA
HaeII 1028 GGCGCC [GA]GCGC^[CT]
HapII 939 CCGG C^CGG
HhaI 1029 GCGC GCG^C
Hin1I 1028 GGCGCC G[GA]^CG[CT]C
HinP1I 1029 GCGC G^CGC
HincII 1297 GTCGAC GT[CT]^[GA]AC
HindII 1297 GTCGAC GT[CT]^[GA]AC
HinfI 1072 GAGTC G^A[ACGT]TC
HpaII 939 CCGG C^CGG
HphI 968 TCACC GGTGA(8/7)
KasI 1028 GGCGCC G^GCGCC
MamI 1230 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 471 TGGCCA TGG^CCA
MscI 471 TGGCCA TGG^CCA
MslI 834 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 939 CCGG C^CGG
NarI 1028 GGCGCC GG^CGCC
NciI 939 CCGGG CC^[GC]GG
NcoI 578 CCATGG C^CATGG
NdeI 782 CATATG CA^TATG
NspV 1121 TTCGAA TT^CGAA
PflFI 1291 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1072 GAGTC GAGTC(4/5)
PmaCI 916 CACGTG CAC^GTG
PmlI 916 CACGTG CAC^GTG
SalI 1297 GTCGAC G^TCGAC
SfoI 1028 GGCGCC GGC^GCC
SfuI 1121 TTCGAA TT^CGAA
SmlI 44 CTCAAG C^T[CT][GA]AG
SspBI 920 TGTACA T^GTACA
Tth111I 1291 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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BanI 82 GGCACC G^G[CT][GA]CC
1028 GGCGCC
BpmI 1273 CTCCAG CTGGAG(16/14)
167 CTGGAG
BspLU11I 255 ACATGT A^CATGT
923 ACATGT
BspMI 815 ACCTGC ACCTGC(4/8)
1301 ACCTGC
BsrDI 555 CATTGC GCAATG(2/0)
1557 CATTGC
EcoRI 1465 GAATTC G^AATTC
1552 GAATTC
EcoT14I 339 CCAAGG C^C[AT][AT]GG
578 CCATGG
FauI 960 CCCGC CCCGC(4/6)
1015 CCCGC
HgaI 903 GACGC GACGC(5/10)
55 GCGTC
NspI 255 ACATGT [GA]CATG^[CT]
923 ACATGT
StyI 339 CCAAGG C^C[AT][AT]GG
578 CCATGG
Tsp45I 875 GTCAC ^GT[GC]AC
1074 GTCAC
XcmI 315 CCAGCTTGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
578 CCATGGCTGTTCTGG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI BbsI BbvI BfaI
BpuAI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I