Restriction map for
pF1KB8633
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRV FauI FseI FspI
HpaI KspI MamI MfeI MflI
MluI MroI MslI MunI NaeI
NcoI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 1173 GGTACC G^GTACC
AccII 144 CGCG CG^CG
AcsI 541 GAATTC [GA]^AATT[CT]
AcyI 6 GGCGCC G[GA]^CG[CT]C
AflIII 152 ACACGT A^C[GA][CT]GT
AlwNI 1097 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 594 CCTAAGG CC^T[ACGT]AGG
Aor51HI 938 AGCGCT AGC^GCT
ApoI 541 GAATTC [GA]^AATT[CT]
Asp700I 1461 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1173 GGTACC G^GTACC
BalI 472 TGGCCA TGG^CCA
BbeI 6 GGCGCC GGCGC^C
BclI 675 TGATCA T^GATCA
BlpI 424 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 424 GCTGAGC GC^T[ACGT]AGC
BsaHI 6 GGCGCC G[GA]^CG[CT]C
BsgI 56 GTGCAG GTGCAG(16/14)
BspMI 271 GCAGGT ACCTGC(4/8)
BsrDI 819 GCAATG GCAATG(2/0)
BstUI 144 CGCG CG^CG
BstXI 1388 CCATCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 594 CCTAAGG CC^T[ACGT]AGG
CelII 424 GCTGAGC GC^T[ACGT]AGC
DraII 79 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 721 GACATCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 472 TGGCCA [CT]^GGCC[GA]
Eco47III 938 AGCGCT AGC^GCT
Eco81I 594 CCTAAGG CC^T[ACGT]AGG
EcoO109I 79 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 541 GAATTC G^AATTC
EcoT14I 1288 CCTTGG C^C[AT][AT]GG
EcoT22I 884 ATGCAT ATGCA^T
FbaI 675 TGATCA T^GATCA
HgaI 142 GACGC GACGC(5/10)
Hin1I 6 GGCGCC G[GA]^CG[CT]C
HincII 1081 GTTGAC GT[CT]^[GA]AC
HindII 1081 GTTGAC GT[CT]^[GA]AC
KasI 6 GGCGCC G^GCGCC
KpnI 1173 GGTACC GGTAC^C
MluNI 472 TGGCCA TGG^CCA
MscI 472 TGGCCA TGG^CCA
MseI 176 TTAA T^TAA
MvnI 144 CGCG CG^CG
NarI 6 GGCGCC GG^CGCC
NheI 1105 GCTAGC G^CTAGC
NsiI 884 ATGCAT ATGCA^T
NspI 811 GCATGT [GA]CATG^[CT]
PpuMI 79 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1205 GAGCTC GAGCT^C
SfoI 6 GGCGCC GGC^GCC
SspI 1473 AATATT AAT^ATT
StuI 918 AGGCCT AGG^CCT
StyI 1288 CCTTGG C^C[AT][AT]GG
TaqI 1050 TCGA T^CGA
Tru9I 176 TTAA T^TAA
TthHB8I 1050 TCGA T^CGA
XmnI 1461 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 446 GTAC GT^AC
1174 GTAC
AlwI 1258 GATCC GGATC(4/5)
181 GGATC
BbsI 631 GAAGAC GAAGAC(2/6)
644 GTCTTC
BcnI 123 CCCGG CC^[GC]GG
980 CCCGG
BpuAI 631 GAAGAC GAAGAC(2/6)
644 GTCTTC
BseRI 962 CTCCTC GAGGAG(10/8)
1218 CTCCTC
EarI 1068 CTCTTC CTCTTC(1/4)
127 GAAGAG
HindIII 362 AAGCTT A^AGCTT
1248 AAGCTT
Ksp632I 1068 CTCTTC CTCTTC(1/4)
127 GAAGAG
MaeII 154 ACGT A^CGT
734 ACGT
MspA1I 100 CAGCTG C[AC]G^C[GT]G
1421 CAGCTG
NciI 123 CCCGG CC^[GC]GG
980 CCCGG
PvuII 100 CAGCTG CAG^CTG
1421 CAGCTG
RsaI 446 GTAC GT^AC
1174 GTAC
SmlI 343 CTCAAG C^T[CT][GA]AG
922 CTTGAG
XcmI 168 CCAGTCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
869 CCATGACTGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AspHI AvaII BanI
BanII BbvI BfaI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI TfiI
TseI Tsp45I Tsp509I TspRI