Restriction map for
pF1KB8641
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglI BglII BlnI BsaBI
BsaHI BsiEI BsiWI Bsp1407I BspDI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI FauI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NarI
NciI NcoI NdeI NheI NotI
NruI NspV PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 408 TCCGGA T^CCGGA
AflIII 2297 ACATGT A^C[GA][CT]GT
AocI 2077 CCTGAGG CC^T[ACGT]AGG
AseI 1350 ATTAAT AT^TAAT
AsnI 1350 ATTAAT AT^TAAT
Asp700I 1722 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1229 TGGCCA TGG^CCA
BlpI 1447 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1447 GCTGAGC GC^T[ACGT]AGC
BsaAI 1994 CACGTA [CT]AC^GT[GA]
BseAI 408 TCCGGA T^CCGGA
BsmBI 1681 GAGACG CGTCTC(1/5)
BsmI 783 GAATGC GAATGC(1/-1)
BspEI 408 TCCGGA T^CCGGA
BspHI 552 TCATGA T^CATGA
BspLU11I 2297 ACATGT A^CATGT
BsrDI 890 CATTGC GCAATG(2/0)
Bsu36I 2077 CCTGAGG CC^T[ACGT]AGG
CelII 1447 GCTGAGC GC^T[ACGT]AGC
DsaI 1673 CCACGG C^C[GA][CT]GG
Eco81I 2077 CCTGAGG CC^T[ACGT]AGG
EcoT22I 887 ATGCAT ATGCA^T
HaeII 1129 AGCGCC [GA]GCGC^[CT]
MluNI 1229 TGGCCA TGG^CCA
MroI 408 TCCGGA T^CCGGA
MscI 1229 TGGCCA TGG^CCA
NaeI 1337 GCCGGC GCC^GGC
NgoMIV 1337 GCCGGC G^CCGGC
NsiI 887 ATGCAT ATGCA^T
PacI 1351 TTAATTAA TTAAT^TAA
PshBI 1350 ATTAAT AT^TAAT
RcaI 552 TCATGA T^CATGA
SacI 1450 GAGCTC GAGCT^C
SphI 1851 GCATGC GCATG^C
StuI 2052 AGGCCT AGG^CCT
XmnI 1722 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 49 AAATTT [GA]^AATT[CT]
349 GAATTT
AlwNI 2264 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2392 CAGCCACTG
ApoI 49 AAATTT [GA]^AATT[CT]
349 GAATTT
BanII 1450 GAGCTC G[GA]GC[CT]^C
1813 GGGCTC
BciVI 1755 GTATCC GTATCC(6/5)
2424 GTATCC
BsaWI 408 TCCGGA [AT]^CCGG[AT]
1421 ACCGGA
BsmFI 1990 GTCCC GGGAC(10/14)
2388 GTCCC
BspMI 672 ACCTGC ACCTGC(4/8)
2381 ACCTGC
BsrFI 603 GCCGGT [GA]^CCGG[CT]
1337 GCCGGC
BstAPI 1290 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2263 GCAGCAGCTGC
BstXI 1577 CCAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2016 CCACCGAAGTGG
BstYI 363 AGATCC [GA]^GATC[CT]
1700 AGATCC
CfoI 1130 GCGC GCG^C
2376 GCGC
Cfr10I 603 GCCGGT [GA]^CCGG[CT]
1337 GCCGGC
EaeI 1196 TGGCCG [CT]^GGCC[GA]
1229 TGGCCA
EcoRV 1831 GATATC GAT^ATC
2131 GATATC
HgaI 1683 GACGC GACGC(5/10)
1155 GCGTC
HhaI 1130 GCGC GCG^C
2376 GCGC
HinP1I 1130 GCGC G^CGC
2376 GCGC
MaeII 1995 ACGT A^CGT
2334 ACGT
MflI 363 AGATCC [GA]^GATC[CT]
1700 AGATCC
MspA1I 1459 CAGCTG C[AC]G^C[GT]G
2267 CAGCTG
PaeR7I 1187 CTCGAG C^TCGAG
2036 CTCGAG
PpuMI 584 AGGACCT [GA]G^G[AT]CC[CT]
1988 AGGTCCC
PvuII 1459 CAGCTG CAG^CTG
2267 CAGCTG
SexAI 437 ACCTGGT A^CC[AT]GGT
1985 ACCAGGT
Tsp45I 683 GTGAC ^GT[GC]AC
998 GTGAC
XhoI 1187 CTCGAG C^TCGAG
2036 CTCGAG
XhoII 363 AGATCC [GA]^GATC[CT]
1700 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanI BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XcmI