Restriction map for
pF1KB8650
##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
Eco47III Eco81I EcoNI EcoRI EcoT14I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 385 GTAGAC GT^[AC][GT]AC
AhdI 481 GACCGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 561 GGATC GGATC(4/5)
AlwNI 634 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 824 GGGCCC GGGCC^C
AspEI 481 GACCGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 258 GAGCTC G[AT]GC[AT]^C
BbsI 451 GAAGAC GAAGAC(2/6)
BglI 695 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 425 AGATCT A^GATCT
BpuAI 451 GAAGAC GAAGAC(2/6)
BsaAI 970 TACGTG [CT]AC^GT[GA]
BsiEI 930 CGGCCG CG[GA][CT]^CG
BsiHKAI 258 GAGCTC G[AT]GC[AT]^C
BsmI 756 GAATGC GAATGC(1/-1)
BstEII 460 GGTCACC G^GT[ACGT]ACC
BstPI 460 GGTCACC G^GT[ACGT]ACC
BstYI 425 AGATCT [GA]^GATC[CT]
EaeI 930 CGGCCG [CT]^GGCC[GA]
EagI 930 CGGCCG C^GGCCG
Eam1105I 481 GACCGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 955 CTCTTC CTCTTC(1/4)
EclXI 930 CGGCCG C^GGCCG
Eco52I 930 CGGCCG C^GGCCG
EcoO65I 460 GGTCACC G^GT[ACGT]ACC
EcoRV 662 GATATC GAT^ATC
EcoT22I 769 ATGCAT ATGCA^T
FauI 881 GCGGG CCCGC(4/6)
Ksp632I 955 CTCTTC CTCTTC(1/4)
MflI 425 AGATCT [GA]^GATC[CT]
NsiI 769 ATGCAT ATGCA^T
PshAI 454 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 637 CAGCTG CAG^CTG
SacI 258 GAGCTC GAGCT^C
SfaNI 771 GCATC GCATC(5/9)
SmaI 827 CCCGGG CCC^GGG
StuI 183 AGGCCT AGG^CCT
Tsp45I 461 GTCAC ^GT[GC]AC
XhoII 425 AGATCT [GA]^GATC[CT]
XmaCI 827 CCCGGG C^CCGGG
XmaI 827 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 144 GAATTT [GA]^AATT[CT]
402 AAATTC
ApoI 144 GAATTT [GA]^AATT[CT]
402 AAATTC
AvaI 446 CCCGAG C^[CT]CG[GA]G
827 CCCGGG
BanI 733 GGCACC G^G[CT][GA]CC
975 GGCACC
BanII 258 GAGCTC G[GA]GC[CT]^C
824 GGGCCC
BsaWI 482 ACCGGA [AT]^CCGG[AT]
548 ACCGGA
BseRI 94 GAGGAG GAGGAG(10/8)
698 GAGGAG
BsgI 937 CTGCAC GTGCAG(16/14)
793 GTGCAG
BsiYI 507 CCTCAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
876 CCACAGCGGGG
BslI 507 CCTCAGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
876 CCACAGCGGGG
BsmFI 269 GGGAC GGGAC(10/14)
489 GTCCC
BsoBI 446 CCCGAG C^[CT]CG[GA]G
827 CCCGGG
BsrFI 736 ACCGGC [GA]^CCGG[CT]
928 ACCGGC
BstXI 916 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
965 CCAACTACGTGG
Cac8I 250 GCCAGC GC[ACGT]^[ACGT]GC
935 GCCTGC
CfoI 628 GCGC GCG^C
832 GCGC
Cfr10I 736 ACCGGC [GA]^CCGG[CT]
928 ACCGGC
DpnII 426 GATC ^GATC
562 GATC
HaeII 627 AGCGCC [GA]GCGC^[CT]
831 GGCGCT
HhaI 628 GCGC GCG^C
832 GCGC
HinP1I 628 GCGC G^CGC
832 GCGC
MaeII 302 ACGT A^CGT
971 ACGT
MaeIII 86 GTAAC ^GT[ACGT]AC
461 GTCAC
MboI 426 GATC ^GATC
562 GATC
MseI 82 TTAA T^TAA
104 TTAA
MspA1I 637 CAGCTG C[AC]G^C[GT]G
879 CAGCGG
NdeII 426 GATC ^GATC
562 GATC
PpuMI 487 AGGTCCC [GA]G^G[AT]CC[CT]
896 AGGTCCT
Sau3AI 426 GATC ^GATC
562 GATC
TaqI 192 TCGA T^CGA
541 TCGA
Tru9I 82 TTAA T^TAA
104 TTAA
TthHB8I 192 TCGA T^CGA
541 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BmyI BpmI BsaJI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MboII MnlI MslI MspI
MvaI MwoI NciI NlaIII NlaIV
PstI Sau96I ScrFI SfcI TseI
Tsp509I TspRI