Restriction map for
pF1KB8654
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AlwI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AviII BamHI BbvCI BciVI BclI
BfrI BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
KpnI MamI MroI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII ScaI SfiI SfuI
SnaBI SpeI SspBI StuI SwaI
XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 162 GACGTC GACGT^C
AccI 1789 GTCGAC GT^[AC][GT]AC
AhdI 2554 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1633 GTGCAC G^TGCAC
ApaLI 1633 GTGCAC G^TGCAC
AspEI 2554 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2410 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 498 CCTAGG C^CTAGG
BbeI 1595 GGCGCC GGCGC^C
BbrPI 178 CACGTG CAC^GTG
BcgI 1791 CGACTCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1186 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 757 AGATCT A^GATCT
BlnI 498 CCTAGG C^CTAGG
BsaAI 178 CACGTG [CT]AC^GT[GA]
BseRI 1390 GAGGAG GAGGAG(10/8)
BsmBI 2380 GAGACG CGTCTC(1/5)
BsmI 571 GCATTC GAATGC(1/-1)
BsrDI 1797 CATTGC GCAATG(2/0)
BssSI 115 CACGAG CACGAG(-5/-1)
BstYI 757 AGATCT [GA]^GATC[CT]
DraI 819 TTTAAA TTT^AAA
Eam1105I 2554 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1479 CCTGACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HpaI 739 GTTAAC GTT^AAC
KasI 1595 GGCGCC G^GCGCC
MfeI 47 CAATTG C^AATTG
MflI 757 AGATCT [GA]^GATC[CT]
MluI 1211 ACGCGT A^CGCGT
MunI 47 CAATTG C^AATTG
NarI 1595 GGCGCC GG^CGCC
PflFI 2410 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 178 CACGTG CAC^GTG
PmlI 178 CACGTG CAC^GTG
PshAI 162 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1699 CAGCTG CAG^CTG
SalI 1789 GTCGAC G^TCGAC
SbfI 303 CCTGCAGG CCTGCA^GG
SexAI 577 ACCAGGT A^CC[AT]GGT
SfoI 1595 GGCGCC GGC^GCC
SgrAI 1149 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2370 CCCGGG CCC^GGG
SmlI 1603 CTCAAG C^T[CT][GA]AG
Sse8387I 303 CCTGCAGG CCTGCA^GG
SspI 593 AATATT AAT^ATT
Tth111I 2410 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 64 TCTAGA T^CTAGA
XhoII 757 AGATCT [GA]^GATC[CT]
XmaCI 2370 CCCGGG C^CCGGG
XmaI 2370 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 1211 ACGCGT A^C[GA][CT]GT
1760 ACGTGT
ApaI 1306 GGGCCC GGGCC^C
2220 GGGCCC
BalI 138 TGGCCA TGG^CCA
926 TGGCCA
BanI 1595 GGCGCC G^G[CT][GA]CC
1966 GGCACC
BfaI 65 CTAG C^TAG
499 CTAG
BlpI 973 GCTGAGC GC^T[ACGT]AGC
2151 GCTGAGC
Bpu1102I 973 GCTGAGC GC^T[ACGT]AGC
2151 GCTGAGC
BsgI 1921 CTGCAC GTGCAG(16/14)
1685 GTGCAG
BspHI 407 TCATGA T^CATGA
1889 TCATGA
BsrFI 1150 GCCGGC [GA]^CCGG[CT]
1598 GCCGGC
CelII 973 GCTGAGC GC^T[ACGT]AGC
2151 GCTGAGC
Cfr10I 1150 GCCGGC [GA]^CCGG[CT]
1598 GCCGGC
DraIII 1865 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2284 CACCGCGTG
EarI 933 CTCTTC CTCTTC(1/4)
1315 CTCTTC
HaeII 522 GGCGCT [GA]GCGC^[CT]
1595 GGCGCC
HincII 739 GTTAAC GT[CT]^[GA]AC
1789 GTCGAC
HindII 739 GTTAAC GT[CT]^[GA]AC
1789 GTCGAC
Ksp632I 933 CTCTTC CTCTTC(1/4)
1315 CTCTTC
KspI 1132 CCGCGG CCGC^GG
2469 CCGCGG
MaeI 65 CTAG C^TAG
499 CTAG
MluNI 138 TGGCCA TGG^CCA
926 TGGCCA
MscI 138 TGGCCA TGG^CCA
926 TGGCCA
MseI 740 TTAA T^TAA
820 TTAA
NaeI 1150 GCCGGC GCC^GGC
1598 GCCGGC
NcoI 420 CCATGG C^CATGG
488 CCATGG
NgoMIV 1150 GCCGGC G^CCGGC
1598 GCCGGC
NspI 2156 GCATGC [GA]CATG^[CT]
2442 GCATGC
PleI 654 GACTC GAGTC(4/5)
1792 GACTC
PpuMI 127 GGGACCT [GA]G^G[AT]CC[CT]
1739 AGGTCCT
RcaI 407 TCATGA T^CATGA
1889 TCATGA
SacII 1132 CCGCGG CCGC^GG
2469 CCGCGG
SapI 932 GCTCTTC GCTCTTC(1/4)
1314 GCTCTTC
SphI 2156 GCATGC GCATG^C
2442 GCATGC
TfiI 560 GAATC G^A[AT]TC
668 GATTC
Tru9I 740 TTAA T^TAA
820 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI