Restriction map for
pF1KB8658
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbvCI BbvI
BcgI BfrI BglI BlnI BsaBI
BseAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
MspA1I MvnI NarI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TseI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 71 enzymes) #
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AatII 1424 GACGTC GACGT^C
AcyI 1424 GACGTC G[GA]^CG[CT]C
AlwNI 299 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 394 ATTAAT AT^TAAT
AsnI 394 ATTAAT AT^TAAT
BanII 866 GAGCTC G[GA]GC[CT]^C
BbrPI 1040 CACGTG CAC^GTG
BbsI 1404 GTCTTC GAAGAC(2/6)
BciVI 536 GGATAC GTATCC(6/5)
BclI 1245 TGATCA T^GATCA
BcnI 949 CCGGG CC^[GC]GG
BglII 808 AGATCT A^GATCT
BlpI 36 GCTGAGC GC^T[ACGT]AGC
BpmI 860 CTCCAG CTGGAG(16/14)
Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC
BpuAI 1404 GTCTTC GAAGAC(2/6)
BsaAI 1040 CACGTG [CT]AC^GT[GA]
BsaHI 1424 GACGTC G[GA]^CG[CT]C
BsaWI 49 ACCGGA [AT]^CCGG[AT]
BsgI 1037 CTGCAC GTGCAG(16/14)
BsiEI 5 CGGCCG CG[GA][CT]^CG
BspMI 1551 ACCTGC ACCTGC(4/8)
BsrBI 39 GAGCGG CCGCTC(-3/-3)
BsrDI 1058 GCAATG GCAATG(2/0)
BsrFI 7 GCCGGC [GA]^CCGG[CT]
BstF5I 57 GGATG GGATG(2/0)
BstXI 1074 CCATCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 808 AGATCT [GA]^GATC[CT]
CelII 36 GCTGAGC GC^T[ACGT]AGC
Cfr10I 7 GCCGGC [GA]^CCGG[CT]
DraII 794 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1259 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 445 CCATGG C^C[GA][CT]GG
EaeI 5 CGGCCG [CT]^GGCC[GA]
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoNI 961 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 794 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 445 CCATGG C^C[AT][AT]GG
FbaI 1245 TGATCA T^GATCA
Fnu4HI 41 GCGGC GC^[ACGT]GC
FokI 57 GGATG GGATG(9/13)
HgaI 1326 GCGTC GACGC(5/10)
Hin1I 1424 GACGTC G[GA]^CG[CT]C
HindIII 512 AAGCTT A^AGCTT
HphI 879 TCACC GGTGA(8/7)
ItaI 41 GCGGC GC^[ACGT]GC
MfeI 716 CAATTG C^AATTG
MflI 808 AGATCT [GA]^GATC[CT]
MslI 1432 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 716 CAATTG C^AATTG
MwoI 1546 GCTACACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 7 GCCGGC GCC^GGC
NciI 949 CCGGG CC^[GC]GG
NcoI 445 CCATGG C^CATGG
NdeI 1052 CATATG CA^TATG
NgoMIV 7 GCCGGC G^CCGGC
NlaIV 46 GGGACC GG[ACGT]^[ACGT]CC
PflMI 277 CCACAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1040 CACGTG CAC^GTG
PmlI 1040 CACGTG CAC^GTG
PpuMI 794 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 394 ATTAAT AT^TAAT
PstI 1553 CTGCAG CTGCA^G
SacI 866 GAGCTC GAGCT^C
SexAI 456 ACCTGGT A^CC[AT]GGT
SfaNI 379 GATGC GCATC(5/9)
StyI 445 CCATGG C^C[AT][AT]GG
Van91I 277 CCACAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 808 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 47 GGACC G^G[AT]CC
795 GGTCC
BfaI 25 CTAG C^TAG
1103 CTAG
BsaI 193 GAGACC GGTCTC(1/5)
1190 GAGACC
BsaJI 445 CCATGG C^C[ACGT][ACGT]GG
709 CCTGGG
CfoI 21 GCGC GCG^C
971 GCGC
Cfr13I 47 GGACC G^G[ACGT]CC
795 GGTCC
FauI 44 GCGGG CCCGC(4/6)
1507 GCGGG
HaeII 20 AGCGCC [GA]GCGC^[CT]
970 GGCGCT
HhaI 21 GCGC GCG^C
971 GCGC
HinP1I 21 GCGC G^CGC
971 GCGC
HinfI 427 GATTC G^A[ACGT]TC
939 GATTC
MaeI 25 CTAG C^TAG
1103 CTAG
MaeII 1041 ACGT A^CGT
1425 ACGT
PshAI 354 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1397 GACATAAGTC
Sau96I 47 GGACC G^G[ACGT]CC
795 GGTCC
TfiI 427 GATTC G^A[AT]TC
939 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccI AciI AfaI AluI AspHI
BmyI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I DdeI DpnII EarI
EcoRII HaeIII HapII HpaII Ksp632I
MaeIII MboI MboII MnlI MseI
MspI MvaI NdeII NlaIII RsaI
Sau3AI ScrFI SfcI TaqI Tru9I
Tsp45I Tsp509I TspRI TthHB8I