Restriction map for
pF1KB8661
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspHI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI StuI StyI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 773 TCCGGA T^CCGGA
AfaI 345 GTAC GT^AC
AlwNI 956 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 976 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 170 GGTGCC G^G[CT][GA]CC
BbsI 198 GAAGAC GAAGAC(2/6)
BclI 582 TGATCA T^GATCA
BcnI 452 CCCGG CC^[GC]GG
BpuAI 198 GAAGAC GAAGAC(2/6)
BsaWI 773 TCCGGA [AT]^CCGG[AT]
BseAI 773 TCCGGA T^CCGGA
BsgI 790 GTGCAG GTGCAG(16/14)
BsmFI 1161 GGGAC GGGAC(10/14)
BsmI 130 GAATGC GAATGC(1/-1)
BspEI 773 TCCGGA T^CCGGA
DraI 307 TTTAAA TTT^AAA
DraII 1214 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 1001 CTCTTC CTCTTC(1/4)
EcoO109I 1214 AGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 582 TGATCA T^GATCA
HgaI 292 GACGC GACGC(5/10)
HincII 334 GTCAAC GT[CT]^[GA]AC
HindII 334 GTCAAC GT[CT]^[GA]AC
HindIII 634 AAGCTT A^AGCTT
Ksp632I 1001 CTCTTC CTCTTC(1/4)
MroI 773 TCCGGA T^CCGGA
MslI 783 CACTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1073 CAGCTG C[AC]G^C[GT]G
NciI 452 CCCGG CC^[GC]GG
PpuMI 1214 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1073 CAGCTG CAG^CTG
RsaI 345 GTAC GT^AC
SapI 1000 GCTCTTC GCTCTTC(1/4)
ScaI 344 AGTACT AGT^ACT
SfaNI 49 GATGC GCATC(5/9)
SspI 589 AATATT AAT^ATT
TaqI 480 TCGA T^CGA
Tsp509I 227 AATT ^AATT
TthHB8I 480 TCGA T^CGA
XcmI 72 CCAGACATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 976 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 76 ACATGT A^C[GA][CT]GT
1145 ACATGT
AspHI 998 GAGCTC G[AT]GC[AT]^C
1232 GAGCTC
BsiHKAI 998 GAGCTC G[AT]GC[AT]^C
1232 GAGCTC
BsmAI 733 GAGAC GTCTC(1/5)
795 GAGAC
BspLU11I 76 ACATGT A^CATGT
1145 ACATGT
BspMI 217 ACCTGC ACCTGC(4/8)
247 ACCTGC
BsrDI 1086 CATTGC GCAATG(2/0)
1110 CATTGC
EaeI 394 CGGCCA [CT]^GGCC[GA]
882 TGGCCG
EcoNI 26 CCTACAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
979 CCTGTTCCAGG
FauI 645 GCGGG CCCGC(4/6)
1182 GCGGG
HapII 453 CCGG C^CGG
774 CCGG
HpaII 453 CCGG C^CGG
774 CCGG
MaeIII 290 GTGAC ^GT[ACGT]AC
431 GTGAC
MspI 453 CCGG C^CGG
774 CCGG
PflMI 1129 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1152 CCACCCAATGG
PleI 958 GACTC GAGTC(4/5)
179 GAGTC
SacI 998 GAGCTC GAGCT^C
1232 GAGCTC
SphI 185 GCATGC GCATG^C
404 GCATGC
TfiI 477 GAATC G^A[AT]TC
611 GAATC
Tsp45I 290 GTGAC ^GT[GC]AC
431 GTGAC
Van91I 1129 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1152 CCACCCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI PstI Sau3AI Sau96I
ScrFI SfcI Tru9I TseI TspRI
XhoII