Restriction map for
pF1KB8665
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI AspEI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI KspI MaeI
MaeII MamI MfeI MluI MluNI
MscI MseI MunI NarI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI RsrII SacII SalI SapI
SbfI SexAI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 1073 GGTACC G^GTACC
AccI 789 GTCTAC GT^[AC][GT]AC
AccIII 1112 TCCGGA T^CCGGA
AcsI 979 AAATTC [GA]^AATT[CT]
AcyI 1156 GACGCC G[GA]^CG[CT]C
Alw44I 894 GTGCAC G^TGCAC
ApaI 758 GGGCCC GGGCC^C
ApaLI 894 GTGCAC G^TGCAC
ApoI 979 AAATTC [GA]^AATT[CT]
Asp700I 1431 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1073 GGTACC G^GTACC
AspI 771 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1417 GCTGAGG CCTCAGC(-5/-2)
BsaHI 1156 GACGCC G[GA]^CG[CT]C
BseAI 1112 TCCGGA T^CCGGA
BspEI 1112 TCCGGA T^CCGGA
BssSI 925 CACGAG CACGAG(-5/-1)
BstAPI 840 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoNI 720 CCTCTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 579 GGCGCT [GA]GCGC^[CT]
Hin1I 1156 GACGCC G[GA]^CG[CT]C
HindIII 227 AAGCTT A^AGCTT
KpnI 1073 GGTACC GGTAC^C
MroI 1112 TCCGGA T^CCGGA
NaeI 703 GCCGGC GCC^GGC
NgoMIV 703 GCCGGC G^CCGGC
NotI 1064 GCGGCCGC GC^GGCCGC
PflFI 771 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1169 AGGACCT [GA]G^G[AT]CC[CT]
PvuI 92 CGATCG CGAT^CG
SacI 16 GAGCTC GAGCT^C
ScaI 350 AGTACT AGT^ACT
SfiI 1242 GGCCACCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1459 CTCAAG C^T[CT][GA]AG
StuI 566 AGGCCT AGG^CCT
Tsp509I 980 AATT ^AATT
Tth111I 771 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 890 CCAAGTGCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1431 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 351 GTAC GT^AC
1074 GTAC
AvaI 754 CTCGGG C^[CT]CG[GA]G
1335 CTCGGG
BanI 729 GGTGCC G^G[CT][GA]CC
1073 GGTACC
BanII 16 GAGCTC G[GA]GC[CT]^C
758 GGGCCC
BglI 1190 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1243 GCCACCAAGGC
BsaWI 852 ACCGGA [AT]^CCGG[AT]
1112 TCCGGA
BsgI 103 GTGCAG GTGCAG(16/14)
838 GTGCAG
BsmAI 112 GAGAC GTCTC(1/5)
3 GTCTC
BsoBI 754 CTCGGG C^[CT]CG[GA]G
1335 CTCGGG
BsrFI 703 GCCGGC [GA]^CCGG[CT]
811 ACCGGC
Cfr10I 703 GCCGGC [GA]^CCGG[CT]
811 ACCGGC
DsaI 299 CCGTGG C^C[GA][CT]GG
677 CCACGG
EagI 680 CGGCCG C^GGCCG
1065 CGGCCG
EclXI 680 CGGCCG C^GGCCG
1065 CGGCCG
Eco52I 680 CGGCCG C^GGCCG
1065 CGGCCG
FauI 733 CCCGC CCCGC(4/6)
1307 GCGGG
MaeIII 505 GTCAC ^GT[ACGT]AC
830 GTGAC
MslI 486 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
693 CATGGGTGTG
PflMI 155 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1371 CCAGGGCCTGG
PleI 376 GAGTC GAGTC(4/5)
625 GAGTC
PvuII 118 CAGCTG CAG^CTG
532 CAGCTG
RsaI 351 GTAC GT^AC
1074 GTAC
Tsp45I 505 GTCAC ^GT[GC]AC
830 GTGAC
Van91I 155 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1371 CCAGGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI TspRI
TthHB8I XhoII