Restriction map for
pF1KB8666
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspMI BsrDI BsrFI BsrGI
BssHII Bst1107I BstEII BstPI BstYI
BstZ17I CelII Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MroI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 919 CGCG CG^CG
AflIII 969 ACATGT A^C[GA][CT]GT
AlwI 247 GATCC GGATC(4/5)
Aor51HI 344 AGCGCT AGC^GCT
ApaI 542 GGGCCC GGGCC^C
AvaI 524 CCCGAG C^[CT]CG[GA]G
BcnI 861 CCGGG CC^[GC]GG
BpmI 588 CTGGAG CTGGAG(16/14)
BsaI 865 GAGACC GGTCTC(1/5)
BsaJI 1013 CCGTGG C^C[ACGT][ACGT]GG
BseRI 1003 GAGGAG GAGGAG(10/8)
BsmFI 362 GGGAC GGGAC(10/14)
BsoBI 524 CCCGAG C^[CT]CG[GA]G
BspDI 751 ATCGAT AT^CGAT
BspLU11I 969 ACATGT A^CATGT
BsrBI 1053 CCGCTC CCGCTC(-3/-3)
BssSI 871 CACGAG CACGAG(-5/-1)
BstBI 92 TTCGAA TT^CGAA
BstNI 888 CCTGG CC^[AT]GG
BstUI 919 CGCG CG^CG
ClaI 751 ATCGAT AT^CGAT
DraII 541 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 1013 CCGTGG C^C[GA][CT]GG
Eco47III 344 AGCGCT AGC^GCT
EcoO109I 541 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 397 GAATTC G^AATTC
EcoRII 888 CCTGG ^CC[AT]GG
FauI 545 CCCGC CCCGC(4/6)
HaeII 344 AGCGCT [GA]GCGC^[CT]
HapII 861 CCGG C^CGG
HincII 264 GTTGAC GT[CT]^[GA]AC
HindII 264 GTTGAC GT[CT]^[GA]AC
HpaII 861 CCGG C^CGG
HphI 336 GGTGA GGTGA(8/7)
MspA1I 902 CCGCTG C[AC]G^C[GT]G
MspI 861 CCGG C^CGG
MvaI 888 CCTGG CC^[AT]GG
MvnI 919 CGCG CG^CG
NciI 861 CCGGG CC^[GC]GG
NspV 92 TTCGAA TT^CGAA
SacI 736 GAGCTC GAGCT^C
SapI 733 GAAGAGC GCTCTTC(1/4)
SfcI 501 CTGTAG C^T[GA][CT]AG
SfuI 92 TTCGAA TT^CGAA
StuI 1024 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 397 GAATTC [GA]^AATT[CT]
667 AAATTC
AluI 404 AGCT AG^CT
737 AGCT
AlwNI 415 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
850 CAGGCTCTG
AocI 412 CCTCAGG CC^T[ACGT]AGG
624 CCTGAGG
ApoI 397 GAATTC [GA]^AATT[CT]
667 AAATTC
Asp700I 95 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
235 GAATATGTTC
BalI 764 TGGCCA TGG^CCA
807 TGGCCA
BanII 542 GGGCCC G[GA]GC[CT]^C
736 GAGCTC
BbsI 220 GAAGAC GAAGAC(2/6)
455 GTCTTC
BbvI 420 GCTGC GCAGC(8/12)
904 GCTGC
BfaI 217 CTAG C^TAG
1000 CTAG
BpuAI 220 GAAGAC GAAGAC(2/6)
455 GTCTTC
BsgI 847 GTGCAG GTGCAG(16/14)
926 GTGCAG
BsiYI 354 CCTGCAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
843 CCAAGTGCAGG
BslI 354 CCTGCAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
843 CCAAGTGCAGG
BsmAI 865 GAGAC GTCTC(1/5)
1019 GAGAC
BssKI 861 CCGGG ^CC[ACGT]GG
888 CCTGG
BstF5I 705 CATCC GGATG(2/0)
595 GGATG
BstXI 881 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
941 CCAGAGAGATGG
Bsu36I 412 CCTCAGG CC^T[ACGT]AGG
624 CCTGAGG
EaeI 764 TGGCCA [CT]^GGCC[GA]
807 TGGCCA
EarI 733 GAAGAG CTCTTC(1/4)
823 GAAGAG
Eco81I 412 CCTCAGG CC^T[ACGT]AGG
624 CCTGAGG
FokI 705 CATCC GGATG(9/13)
595 GGATG
Ksp632I 733 GAAGAG CTCTTC(1/4)
823 GAAGAG
MaeI 217 CTAG C^TAG
1000 CTAG
MaeIII 17 GTCAC ^GT[ACGT]AC
568 GTGAC
MluNI 764 TGGCCA TGG^CCA
807 TGGCCA
MscI 764 TGGCCA TGG^CCA
807 TGGCCA
NspI 451 GCATGT [GA]CATG^[CT]
969 ACATGT
PleI 223 GACTC GAGTC(4/5)
364 GACTC
ScrFI 861 CCGGG CC^[ACGT]GG
888 CCTGG
SfaNI 649 GATGC GCATC(5/9)
704 GCATC
TseI 420 GCTGC G^C[AT]GC
904 GCTGC
Tsp45I 17 GTCAC ^GT[GC]AC
568 GTGAC
Tsp509I 398 AATT ^AATT
668 AATT
XmnI 95 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
235 GAATATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AspHI BmyI BsiHKAI
Bsp1286I BsrI BstAPI Cac8I CfoI
Cfr13I DdeI DpnII Fnu4HI HaeIII
HhaI HinP1I HinfI ItaI MboI
MboII MnlI MseI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
TaqI TfiI Tru9I TspRI TthHB8I