Restriction map for
pF1KB8668
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstUI
BstXI CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SpeI Sse8387I SspBI
StyI SwaI Tth111I XbaI XcmI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1053 GTATAC GT^[AC][GT]AC
AcsI 388 GAATTT [GA]^AATT[CT]
AfaI 1363 GTAC GT^AC
AflIII 205 ACATGT A^C[GA][CT]GT
Alw44I 708 GTGCAC G^TGCAC
ApaLI 708 GTGCAC G^TGCAC
ApoI 388 GAATTT [GA]^AATT[CT]
Asp700I 796 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 145 GGACC G^G[AT]CC
BanII 247 GAGCTC G[GA]GC[CT]^C
BbsI 362 GAAGAC GAAGAC(2/6)
BpuAI 362 GAAGAC GAAGAC(2/6)
BsaAI 891 TACGTA [CT]AC^GT[GA]
BsmAI 95 GAGAC GTCTC(1/5)
BsmI 664 GAATGC GAATGC(1/-1)
BspLU11I 205 ACATGT A^CATGT
Bst1107I 1053 GTATAC GTA^TAC
BstAPI 952 GCAGTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 166 TTCGAA TT^CGAA
BstEII 1251 GGTAACC G^GT[ACGT]ACC
BstPI 1251 GGTAACC G^GT[ACGT]ACC
BstYI 570 AGATCC [GA]^GATC[CT]
BstZ17I 1053 GTATAC GTA^TAC
Cfr13I 145 GGACC G^G[ACGT]CC
EarI 542 CTCTTC CTCTTC(1/4)
EcoO65I 1251 GGTAACC G^GT[ACGT]ACC
EcoRV 537 GATATC GAT^ATC
Ksp632I 542 CTCTTC CTCTTC(1/4)
MaeII 892 ACGT A^CGT
MflI 570 AGATCC [GA]^GATC[CT]
MspA1I 962 CAGCTG C[AC]G^C[GT]G
NspV 166 TTCGAA TT^CGAA
PaeR7I 133 CTCGAG C^TCGAG
PleI 499 GACTC GAGTC(4/5)
PvuII 962 CAGCTG CAG^CTG
RsaI 1363 GTAC GT^AC
SacI 247 GAGCTC GAGCT^C
Sau96I 145 GGACC G^G[ACGT]CC
SfuI 166 TTCGAA TT^CGAA
SmaI 629 CCCGGG CCC^GGG
SnaBI 891 TACGTA TAC^GTA
SphI 530 GCATGC GCATG^C
StuI 346 AGGCCT AGG^CCT
Tsp45I 1345 GTCAC ^GT[GC]AC
XhoI 133 CTCGAG C^TCGAG
XhoII 570 AGATCC [GA]^GATC[CT]
XmaCI 629 CCCGGG C^CCGGG
XmaI 629 CCCGGG C^CCGGG
XmnI 796 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 571 GATCC GGATC(4/5)
820 GATCC
AocI 367 CCTAAGG CC^T[ACGT]AGG
775 CCTAAGG
AvaI 133 CTCGAG C^[CT]CG[GA]G
629 CCCGGG
BcnI 629 CCCGG CC^[GC]GG
630 CCGGG
BsaJI 629 CCCGGG C^C[ACGT][ACGT]GG
727 CCCAGG
BseRI 13 GAGGAG GAGGAG(10/8)
43 GAGGAG
BsiYI 512 CCAGAAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
805 CCATTAGGTGG
BslI 512 CCAGAAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
805 CCATTAGGTGG
BsoBI 133 CTCGAG C^[CT]CG[GA]G
629 CCCGGG
BstNI 643 CCTGG CC^[AT]GG
728 CCAGG
Bsu36I 367 CCTAAGG CC^T[ACGT]AGG
775 CCTAAGG
DraI 616 TTTAAA TTT^AAA
985 TTTAAA
Eco81I 367 CCTAAGG CC^T[ACGT]AGG
775 CCTAAGG
EcoRII 643 CCTGG ^CC[AT]GG
728 CCAGG
HapII 19 CCGG C^CGG
630 CCGG
HpaII 19 CCGG C^CGG
630 CCGG
HphI 633 GGTGA GGTGA(8/7)
1346 TCACC
MspI 19 CCGG C^CGG
630 CCGG
MvaI 643 CCTGG CC^[AT]GG
728 CCAGG
NciI 629 CCCGG CC^[GC]GG
630 CCGGG
NspI 205 ACATGT [GA]CATG^[CT]
530 GCATGC
PflMI 512 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
805 CCATTAGGTGG
PstI 383 CTGCAG CTGCA^G
932 CTGCAG
SfaNI 718 GCATC GCATC(5/9)
865 GCATC
SmlI 133 CTCGAG C^T[CT][GA]AG
905 CTCAAG
SspI 877 AATATT AAT^ATT
1117 AATATT
Van91I 512 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
805 CCATTAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AspHI BbvI BfaI
BmyI BpmI BsiHKAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I Cac8I
DdeI DpnII Fnu4HI FokI HaeIII
HinfI ItaI MaeI MaeIII MboI
MboII MnlI MseI MwoI NdeII
NlaIII NlaIV Sau3AI ScrFI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I