Restriction map for
pF1KB8673
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspBI StuI SwaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AflIII 953 ACGTGT A^C[GA][CT]GT
AocI 156 CCTGAGG CC^T[ACGT]AGG
Asp700I 1041 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 535 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1763 TGGCCA TGG^CCA
BanII 625 GAGCTC G[GA]GC[CT]^C
BbsI 108 GAAGAC GAAGAC(2/6)
BbvI 42 GCAGC GCAGC(8/12)
BciVI 1077 GTATCC GTATCC(6/5)
BglII 220 AGATCT A^GATCT
BpmI 389 CTCCAG CTGGAG(16/14)
BpuAI 108 GAAGAC GAAGAC(2/6)
BsaI 1203 GAGACC GGTCTC(1/5)
BsaWI 363 ACCGGA [AT]^CCGG[AT]
BsgI 40 GTGCAG GTGCAG(16/14)
BsmI 942 GAATGC GAATGC(1/-1)
BsrBI 1215 CCGCTC CCGCTC(-3/-3)
BsrDI 1010 GCAATG GCAATG(2/0)
BssSI 664 CTCGTG CACGAG(-5/-1)
BstXI 793 CCATATCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 220 AGATCT [GA]^GATC[CT]
Bsu36I 156 CCTGAGG CC^T[ACGT]AGG
DraI 1462 TTTAAA TTT^AAA
DraII 1813 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1250 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1642 GACCCTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 278 CCATGG C^C[GA][CT]GG
Eco81I 156 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1813 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 278 CCATGG C^C[AT][AT]GG
EcoT22I 1366 ATGCAT ATGCA^T
FauI 1818 CCCGC CCCGC(4/6)
Fnu4HI 42 GCAGC GC^[ACGT]GC
HapII 364 CCGG C^CGG
HincII 1015 GTCAAC GT[CT]^[GA]AC
HindII 1015 GTCAAC GT[CT]^[GA]AC
HpaII 364 CCGG C^CGG
ItaI 42 GCAGC GC^[ACGT]GC
MaeII 953 ACGT A^CGT
MflI 220 AGATCT [GA]^GATC[CT]
MluNI 1763 TGGCCA TGG^CCA
MscI 1763 TGGCCA TGG^CCA
MslI 948 CATGAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 364 CCGG C^CGG
NcoI 278 CCATGG C^CATGG
NsiI 1366 ATGCAT ATGCA^T
NspI 1662 GCATGC [GA]CATG^[CT]
PaeR7I 1646 CTCGAG C^TCGAG
PflFI 535 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1605 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1403 CTGCAG CTGCA^G
SacI 625 GAGCTC GAGCT^C
ScaI 619 AGTACT AGT^ACT
SfaNI 1368 GCATC GCATC(5/9)
SmlI 1646 CTCGAG C^T[CT][GA]AG
SpeI 517 ACTAGT A^CTAGT
SphI 1662 GCATGC GCATG^C
SspI 174 AATATT AAT^ATT
StyI 278 CCATGG C^C[AT][AT]GG
TseI 42 GCAGC G^C[AT]GC
Tth111I 535 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1605 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 189 TCTAGA T^CTAGA
XhoI 1646 CTCGAG C^TCGAG
XhoII 220 AGATCT [GA]^GATC[CT]
XmnI 1041 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AciI 1215 CCGC CCGC(-3/-1)
1819 CCGC
AlwI 129 GGATC GGATC(4/5)
1424 GGATC
AspHI 625 GAGCTC G[AT]GC[AT]^C
859 GAGCAC
AvaII 354 GGACC G^G[AT]CC
1056 GGACC
BmyI 625 GAGCTC G[AGT]GC[ACT]^C
859 GAGCAC
BsaJI 278 CCATGG C^C[ACGT][ACGT]GG
1604 CCCAGG
BseRI 53 CTCCTC GAGGAG(10/8)
1274 CTCCTC
BsiHKAI 625 GAGCTC G[AT]GC[AT]^C
859 GAGCAC
Bsp1286I 625 GAGCTC G[AGT]GC[ACT]^C
859 GAGCAC
Cac8I 1662 GCATGC GC[ACGT]^[ACGT]GC
1705 GCAAGC
EaeI 1557 TGGCCG [CT]^GGCC[GA]
1763 TGGCCA
MwoI 1696 GCAAAGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1796 GCAGTCATGGC
SfcI 1050 CTGTAG C^T[GA][CT]AG
1403 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaI
BfaI BsiYI BslI BsmAI BsmFI
BsoBI BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EarI EcoRII
FokI HaeIII HinfI HphI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I