Restriction map for
pF1KB8675
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
MamI MfeI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tru9I Tsp45I Tsp509I
Tth111I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 1327 GTAGAC GT^[AC][GT]AC
Aor51HI 167 AGCGCT AGC^GCT
AviII 1355 TGCGCA TGC^GCA
BbeI 1379 GGCGCC GGCGC^C
BbvCI 1018 GCTGAGG CCTCAGC(-5/-2)
BglI 109 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1055 AGATCT A^GATCT
Bsp1407I 1163 TGTACA T^GTACA
BsrBI 1249 GAGCGG CCGCTC(-3/-3)
BsrGI 1163 TGTACA T^GTACA
BstXI 1826 CCAGCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 1771 GAAGAG CTCTTC(1/4)
Eco47III 167 AGCGCT AGC^GCT
FspI 1355 TGCGCA TGC^GCA
HincII 1342 GTCAAC GT[CT]^[GA]AC
HindII 1342 GTCAAC GT[CT]^[GA]AC
KasI 1379 GGCGCC G^GCGCC
Ksp632I 1771 GAAGAG CTCTTC(1/4)
KspI 19 CCGCGG CCGC^GG
MaeIII 1430 GTAAC ^GT[ACGT]AC
NaeI 139 GCCGGC GCC^GGC
NarI 1379 GGCGCC GG^CGCC
NcoI 1832 CCATGG C^CATGG
NgoMIV 139 GCCGGC G^CCGGC
PflMI 75 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 19 CCGCGG CCGC^GG
SapI 1771 GAAGAGC GCTCTTC(1/4)
SexAI 608 ACCTGGT A^CC[AT]GGT
SfoI 1379 GGCGCC GGC^GCC
SspBI 1163 TGTACA T^GTACA
SspI 1031 AATATT AAT^ATT
StuI 1316 AGGCCT AGG^CCT
TfiI 265 GAATC G^A[AT]TC
Van91I 75 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AatII 472 GACGTC GACGT^C
690 GACGTC
AocI 133 CCTGAGG CC^T[ACGT]AGG
1656 CCTGAGG
ApaI 1075 GGGCCC GGGCC^C
1367 GGGCCC
BfaI 533 CTAG C^TAG
1411 CTAG
BsgI 601 CTGCAC GTGCAG(16/14)
307 GTGCAG
BspHI 416 TCATGA T^CATGA
1188 TCATGA
BspMI 782 ACCTGC ACCTGC(4/8)
291 GCAGGT
BsrFI 139 GCCGGC [GA]^CCGG[CT]
1376 ACCGGC
Bsu36I 133 CCTGAGG CC^T[ACGT]AGG
1656 CCTGAGG
Cfr10I 139 GCCGGC [GA]^CCGG[CT]
1376 ACCGGC
Eco81I 133 CCTGAGG CC^T[ACGT]AGG
1656 CCTGAGG
EcoNI 532 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1128 CCTGAAGCAGG
MaeI 533 CTAG C^TAG
1411 CTAG
MslI 6 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1230 CATCTGGATG
PaeR7I 388 CTCGAG C^TCGAG
1567 CTCGAG
PleI 87 GACTC GAGTC(4/5)
1558 GAGTC
PpuMI 941 AGGTCCT [GA]G^G[AT]CC[CT]
1578 GGGACCT
PstI 358 CTGCAG CTGCA^G
1690 CTGCAG
PvuII 1305 CAGCTG CAG^CTG
1586 CAGCTG
RcaI 416 TCATGA T^CATGA
1188 TCATGA
XcmI 479 CCAAAGACACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1103 CCAAGAACAACCTGG
XhoI 388 CTCGAG C^TCGAG
1567 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI TspRI
TthHB8I XhoII