Restriction map for
pF1KB8676
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccI AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspI AviII BalI BbeI
BbrPI BbvCI BcgI BclI BcnI
BglI BglII BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI NarI NciI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 1197 GGTACC G^GTACC
AccII 326 CGCG CG^CG
AcsI 718 GAATTC [GA]^AATT[CT]
AflII 259 CTTAAG C^TTAAG
AlwNI 1044 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 376 GGGCCC GGGCC^C
ApoI 718 GAATTC [GA]^AATT[CT]
Asp718I 1197 GGTACC G^GTACC
AspHI 968 GAGCAC G[AT]GC[AT]^C
AvaI 833 CCCGAG C^[CT]CG[GA]G
AvrII 208 CCTAGG C^CTAGG
BamHI 938 GGATCC G^GATCC
BbsI 653 GTCTTC GAAGAC(2/6)
BciVI 978 GTATCC GTATCC(6/5)
BfrI 259 CTTAAG C^TTAAG
BlnI 208 CCTAGG C^CTAGG
BpmI 349 CTGGAG CTGGAG(16/14)
BpuAI 653 GTCTTC GAAGAC(2/6)
BseRI 846 CTCCTC GAGGAG(10/8)
BsiHKAI 968 GAGCAC G[AT]GC[AT]^C
BsmI 25 GAATGC GAATGC(1/-1)
BsoBI 833 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1064 TGTACA T^GTACA
BsrDI 189 CATTGC GCAATG(2/0)
BsrFI 1222 GCCGGC [GA]^CCGG[CT]
BsrGI 1064 TGTACA T^GTACA
BstUI 326 CGCG CG^CG
BstYI 938 GGATCC [GA]^GATC[CT]
Cfr10I 1222 GCCGGC [GA]^CCGG[CT]
DraI 444 TTTAAA TTT^AAA
EaeI 1220 TGGCCG [CT]^GGCC[GA]
EcoRI 718 GAATTC G^AATTC
HapII 1223 CCGG C^CGG
HpaII 1223 CCGG C^CGG
KpnI 1197 GGTACC GGTAC^C
KspI 325 CCGCGG CCGC^GG
MflI 938 GGATCC [GA]^GATC[CT]
MspI 1223 CCGG C^CGG
MvnI 326 CGCG CG^CG
NaeI 1222 GCCGGC GCC^GGC
NcoI 744 CCATGG C^CATGG
NgoMIV 1222 GCCGGC G^CCGGC
NheI 907 GCTAGC G^CTAGC
NspI 1259 GCATGC [GA]CATG^[CT]
PleI 960 GACTC GAGTC(4/5)
SacII 325 CCGCGG CCGC^GG
SbfI 723 CCTGCAGG CCTGCA^GG
SexAI 527 ACCTGGT A^CC[AT]GGT
SphI 1259 GCATGC GCATG^C
Sse8387I 723 CCTGCAGG CCTGCA^GG
SspBI 1064 TGTACA T^GTACA
SspI 766 AATATT AAT^ATT
StuI 810 AGGCCT AGG^CCT
TaqI 387 TCGA T^CGA
TfiI 491 GAATC G^A[AT]TC
TthHB8I 387 TCGA T^CGA
XcmI 296 CCACACCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 938 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 939 GATCC GGATC(4/5)
938 GGATC
Asp700I 277 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
886 GAACGGCTTC
BanI 321 GGTGCC G^G[CT][GA]CC
1197 GGTACC
BanII 376 GGGCCC G[GA]GC[CT]^C
460 GGGCTC
BlpI 310 GCTAAGC GC^T[ACGT]AGC
793 GCTAAGC
Bpu1102I 310 GCTAAGC GC^T[ACGT]AGC
793 GCTAAGC
BsaI 900 GAGACC GGTCTC(1/5)
1038 GAGACC
BspHI 1031 TCATGA T^CATGA
1073 TCATGA
BsrI 403 ACTGG ACTGG(1/-1)
1043 CCAGT
CelII 310 GCTAAGC GC^T[ACGT]AGC
793 GCTAAGC
EarI 1243 CTCTTC CTCTTC(1/4)
1035 GAAGAG
EcoT14I 208 CCTAGG C^C[AT][AT]GG
744 CCATGG
FauI 327 GCGGG CCCGC(4/6)
630 GCGGG
HaeII 711 GGCGCT [GA]GCGC^[CT]
1088 GGCGCT
HinfI 491 GAATC G^A[ACGT]TC
960 GACTC
HphI 957 GGTGA GGTGA(8/7)
1114 TCACC
Ksp632I 1243 CTCTTC CTCTTC(1/4)
1035 GAAGAG
MslI 745 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1227 CATCAGAGTG
RcaI 1031 TCATGA T^CATGA
1073 TCATGA
StyI 208 CCTAGG C^C[AT][AT]GG
744 CCATGG
Tsp45I 38 GTGAC ^GT[GC]AC
958 GTGAC
Tsp509I 497 AATT ^AATT
719 AATT
XmnI 277 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
886 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BmyI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I ItaI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
Tru9I TseI TspRI