Restriction map for
pF1KB8677
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbvCI BcgI BfaI BfrI
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BseRI BsgI BsiWI BsmI
BspDI BspEI BspHI BspMI BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 126 GTCGAC GT^[AC][GT]AC
AccII 140 CGCG CG^CG
AcsI 768 GAATTC [GA]^AATT[CT]
AocI 1088 CCTCAGG CC^T[ACGT]AGG
ApoI 768 GAATTC [GA]^AATT[CT]
Asp700I 592 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 685 GAGCTC G[AT]GC[AT]^C
AspI 520 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 638 CTCGGG C^[CT]CG[GA]G
AvaII 50 GGTCC G^G[AT]CC
BbrPI 775 CACGTG CAC^GTG
BciVI 797 GTATCC GTATCC(6/5)
BclI 561 TGATCA T^GATCA
BglI 16 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 445 AGATCT A^GATCT
BsaBI 694 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 232 GAGACC GGTCTC(1/5)
BsiHKAI 685 GAGCTC G[AT]GC[AT]^C
BsoBI 638 CTCGGG C^[CT]CG[GA]G
Bsp1407I 10 TGTACA T^GTACA
BspLU11I 986 ACATGT A^CATGT
BsrGI 10 TGTACA T^GTACA
BssSI 419 CTCGTG CACGAG(-5/-1)
BstUI 140 CGCG CG^CG
BstYI 445 AGATCT [GA]^GATC[CT]
Bsu36I 1088 CCTCAGG CC^T[ACGT]AGG
CfoI 789 GCGC GCG^C
EagI 98 CGGCCG C^GGCCG
EclXI 98 CGGCCG C^GGCCG
Eco52I 98 CGGCCG C^GGCCG
Eco81I 1088 CCTCAGG CC^T[ACGT]AGG
EcoRI 768 GAATTC G^AATTC
FauI 21 GCGGG CCCGC(4/6)
FbaI 561 TGATCA T^GATCA
HaeII 788 GGCGCT [GA]GCGC^[CT]
HhaI 789 GCGC GCG^C
HinP1I 789 GCGC G^CGC
HincII 126 GTCGAC GT[CT]^[GA]AC
HindII 126 GTCGAC GT[CT]^[GA]AC
MamI 694 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 445 AGATCT [GA]^GATC[CT]
MspA1I 42 CCGCTG C[AC]G^C[GT]G
MvnI 140 CGCG CG^CG
NcoI 360 CCATGG C^CATGG
NspI 986 ACATGT [GA]CATG^[CT]
PflFI 520 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 775 CACGTG CAC^GTG
PmlI 775 CACGTG CAC^GTG
SacI 685 GAGCTC GAGCT^C
SalI 126 GTCGAC G^TCGAC
SfaNI 415 GATGC GCATC(5/9)
SspBI 10 TGTACA T^GTACA
StuI 1092 AGGCCT AGG^CCT
TaqI 127 TCGA T^CGA
Tsp509I 769 AATT ^AATT
Tth111I 520 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 127 TCGA T^CGA
XcmI 506 CCATCCCAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 445 AGATCT [GA]^GATC[CT]
XmnI 592 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BalI 76 TGGCCA TGG^CCA
357 TGGCCA
BbsI 535 GAAGAC GAAGAC(2/6)
1077 GAAGAC
BpuAI 535 GAAGAC GAAGAC(2/6)
1077 GAAGAC
BsaAI 722 TACGTG [CT]AC^GT[GA]
775 CACGTG
BsiEI 55 CGGTCG CG[GA][CT]^CG
98 CGGCCG
BsmBI 960 CGTCTC CGTCTC(1/5)
643 GAGACG
BsmFI 51 GTCCC GGGAC(10/14)
156 GTCCC
BsrBI 85 CCGCTC CCGCTC(-3/-3)
841 GAGCGG
DpnII 446 GATC ^GATC
562 GATC
DsaI 32 CCGTGG C^C[GA][CT]GG
360 CCATGG
HinfI 669 GAATC G^A[ACGT]TC
1041 GATTC
HphI 375 GGTGA GGTGA(8/7)
672 TCACC
MboI 446 GATC ^GATC
562 GATC
MluNI 76 TGGCCA TGG^CCA
357 TGGCCA
MscI 76 TGGCCA TGG^CCA
357 TGGCCA
MslI 747 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1027 CACTTTGATG
NdeII 446 GATC ^GATC
562 GATC
PstI 326 CTGCAG CTGCA^G
967 CTGCAG
Sau3AI 446 GATC ^GATC
562 GATC
SfcI 326 CTGCAG C^T[GA][CT]AG
967 CTGCAG
TfiI 669 GAATC G^A[AT]TC
1041 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwNI
ApaI BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HpaII
ItaI MaeII MaeIII MboII MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI StyI
TseI Tsp45I TspRI