Restriction map for
pF1KB8678
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BalI BciVI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BseAI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BsrI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 565 GACGTC GACGT^C
AccII 555 CGCG CG^CG
AocI 997 CCTCAGG CC^T[ACGT]AGG
ApaI 770 GGGCCC GGGCC^C
AviII 542 TGCGCA TGC^GCA
BamHI 49 GGATCC G^GATCC
BbeI 336 GGCGCC GGCGC^C
BbrPI 1017 CACGTG CAC^GTG
BbsI 1093 GAAGAC GAAGAC(2/6)
BbvCI 184 GCTGAGG CCTCAGC(-5/-2)
BcgI 545 GCATCGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 81 TGATCA T^GATCA
BpuAI 1093 GAAGAC GAAGAC(2/6)
BsaAI 1017 CACGTG [CT]AC^GT[GA]
BsaWI 52 TCCGGT [AT]^CCGG[AT]
BsgI 992 CTGCAC GTGCAG(16/14)
BsmFI 563 GGGAC GGGAC(10/14)
BsrBI 404 CCGCTC CCGCTC(-3/-3)
BssSI 826 CACGAG CACGAG(-5/-1)
BstAPI 315 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 555 CGCG CG^CG
BstXI 458 CCACAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 997 CCTCAGG CC^T[ACGT]AGG
DraI 342 TTTAAA TTT^AAA
DsaI 221 CCATGG C^C[GA][CT]GG
EarI 784 CTCTTC CTCTTC(1/4)
Eco81I 997 CCTCAGG CC^T[ACGT]AGG
EcoRV 987 GATATC GAT^ATC
EcoT14I 221 CCATGG C^C[AT][AT]GG
FbaI 81 TGATCA T^GATCA
FspI 542 TGCGCA TGC^GCA
HaeII 336 GGCGCC [GA]GCGC^[CT]
HgaI 696 GACGC GACGC(5/10)
KasI 336 GGCGCC G^GCGCC
Ksp632I 784 CTCTTC CTCTTC(1/4)
MspA1I 292 CAGCTG C[AC]G^C[GT]G
MvnI 555 CGCG CG^CG
NarI 336 GGCGCC GG^CGCC
NcoI 221 CCATGG C^CATGG
PmaCI 1017 CACGTG CAC^GTG
PmlI 1017 CACGTG CAC^GTG
PpuMI 830 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 295 CTGCAG CTGCA^G
PvuII 292 CAGCTG CAG^CTG
SapI 783 GCTCTTC GCTCTTC(1/4)
ScaI 719 AGTACT AGT^ACT
SfoI 336 GGCGCC GGC^GCC
StyI 221 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 336 GGCGCC G[GA]^CG[CT]C
565 GACGTC
AfaI 430 GTAC GT^AC
720 GTAC
AspHI 190 GAGCAC G[AT]GC[AT]^C
322 GTGCTC
BanI 336 GGCGCC G^G[CT][GA]CC
1099 GGTGCC
BfaI 700 CTAG C^TAG
961 CTAG
BsaHI 336 GGCGCC G[GA]^CG[CT]C
565 GACGTC
BsaI 388 GAGACC GGTCTC(1/5)
1071 GGTCTC
BsiHKAI 190 GAGCAC G[AT]GC[AT]^C
322 GTGCTC
BsmBI 567 CGTCTC CGTCTC(1/5)
694 GAGACG
BstNI 834 CCTGG CC^[AT]GG
1047 CCTGG
BstYI 49 GGATCC [GA]^GATC[CT]
86 AGATCC
DraII 770 GGGCCCC [GA]G^G[ACGT]CC[CT]
830 AGGTCCT
EcoNI 327 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
591 CCTGCGTAAGG
EcoO109I 770 GGGCCCC [GA]G^G[ACGT]CC[CT]
830 AGGTCCT
EcoRII 834 CCTGG ^CC[AT]GG
1047 CCTGG
FauI 403 CCCGC CCCGC(4/6)
556 GCGGG
Hin1I 336 GGCGCC G[GA]^CG[CT]C
565 GACGTC
MaeI 700 CTAG C^TAG
961 CTAG
MflI 49 GGATCC [GA]^GATC[CT]
86 AGATCC
MvaI 834 CCTGG CC^[AT]GG
1047 CCTGG
PleI 4 GAGTC GAGTC(4/5)
488 GAGTC
RsaI 430 GTAC GT^AC
720 GTAC
SfcI 295 CTGCAG C^T[GA][CT]AG
446 CTACAG
TfiI 475 GATTC G^A[AT]TC
764 GATTC
Tsp45I 490 GTCAC ^GT[GC]AC
932 GTGAC
XhoII 49 GGATCC [GA]^GATC[CT]
86 AGATCC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BspMI
BssKI BstF5I Cac8I CfoI Cfr13I
DdeI DpnII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TaqI
Tru9I TseI TthHB8I