Restriction map for
pF1KB8680
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BsaAI BsaBI BsaI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspMI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FseI FspI HaeII HgaI
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NciI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 1804 GACGTC GACGT^C
AccI 874 GTCGAC GT^[AC][GT]AC
AccII 239 CGCG CG^CG
AccIII 564 TCCGGA T^CCGGA
AcyI 1804 GACGTC G[GA]^CG[CT]C
Alw44I 74 GTGCAC G^TGCAC
AlwI 124 GATCC GGATC(4/5)
AlwNI 131 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 74 GTGCAC G^TGCAC
AspI 868 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 424 CTCGGG C^[CT]CG[GA]G
BalI 320 TGGCCA TGG^CCA
BanI 1859 GGCACC G^G[CT][GA]CC
BfaI 220 CTAG C^TAG
BsaHI 1804 GACGTC G[GA]^CG[CT]C
BseAI 564 TCCGGA T^CCGGA
BsgI 739 GTGCAG GTGCAG(16/14)
BsmAI 984 GTCTC GTCTC(1/5)
BsoBI 424 CTCGGG C^[CT]CG[GA]G
BspEI 564 TCCGGA T^CCGGA
BspLU11I 1073 ACATGT A^CATGT
BsrDI 1340 CATTGC GCAATG(2/0)
BstUI 239 CGCG CG^CG
BstYI 123 AGATCC [GA]^GATC[CT]
CfoI 240 GCGC GCG^C
DraIII 92 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 320 TGGCCA [CT]^GGCC[GA]
EcoT22I 927 ATGCAT ATGCA^T
FauI 527 CCCGC CCCGC(4/6)
HhaI 240 GCGC GCG^C
Hin1I 1804 GACGTC G[GA]^CG[CT]C
HinP1I 240 GCGC G^CGC
HincII 874 GTCGAC GT[CT]^[GA]AC
HindII 874 GTCGAC GT[CT]^[GA]AC
HindIII 409 AAGCTT A^AGCTT
MaeI 220 CTAG C^TAG
MflI 123 AGATCC [GA]^GATC[CT]
MluNI 320 TGGCCA TGG^CCA
MroI 564 TCCGGA T^CCGGA
MscI 320 TGGCCA TGG^CCA
MvnI 239 CGCG CG^CG
NaeI 181 GCCGGC GCC^GGC
NgoMIV 181 GCCGGC G^CCGGC
NsiI 927 ATGCAT ATGCA^T
PflFI 868 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 593 GGGACCT [GA]G^G[AT]CC[CT]
PstI 997 CTGCAG CTGCA^G
SalI 874 GTCGAC G^TCGAC
SapI 1684 GAAGAGC GCTCTTC(1/4)
SmlI 302 CTCAAG C^T[CT][GA]AG
Tsp45I 1328 GTGAC ^GT[GC]AC
Tth111I 868 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 219 TCTAGA T^CTAGA
XhoII 123 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 528 CCGC CCGC(-3/-1)
390 GCGG
AflIII 1073 ACATGT A^C[GA][CT]GT
1578 ACACGT
AocI 766 CCTGAGG CC^T[ACGT]AGG
1465 CCTGAGG
BanII 421 GAGCTC G[GA]GC[CT]^C
439 GAGCTC
BclI 951 TGATCA T^GATCA
1167 TGATCA
BpmI 547 CTCCAG CTGGAG(16/14)
778 CTGGAG
BsaWI 193 TCCGGT [AT]^CCGG[AT]
564 TCCGGA
BspHI 903 TCATGA T^CATGA
954 TCATGA
BsrBI 528 CCGCTC CCGCTC(-3/-3)
388 GAGCGG
BsrFI 181 GCCGGC [GA]^CCGG[CT]
326 ACCGGC
Bsu36I 766 CCTGAGG CC^T[ACGT]AGG
1465 CCTGAGG
Cfr10I 181 GCCGGC [GA]^CCGG[CT]
326 ACCGGC
Eco81I 766 CCTGAGG CC^T[ACGT]AGG
1465 CCTGAGG
FbaI 951 TGATCA T^GATCA
1167 TGATCA
HphI 196 GGTGA GGTGA(8/7)
1045 GGTGA
MseI 493 TTAA T^TAA
689 TTAA
MslI 814 CACCCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1159 CATTGGTGTG
MspA1I 247 CAGCTG C[AC]G^C[GT]G
607 CAGCTG
NspI 1073 ACATGT [GA]CATG^[CT]
1752 ACATGC
PvuII 247 CAGCTG CAG^CTG
607 CAGCTG
RcaI 903 TCATGA T^CATGA
954 TCATGA
SacI 421 GAGCTC GAGCT^C
439 GAGCTC
SfcI 997 CTGCAG C^T[GA][CT]AG
1320 CTACAG
Tru9I 493 TTAA T^TAA
689 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AfaI AluI AspHI AvaII BbsI
BbvCI BbvI BlpI BmyI Bpu1102I
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CelII Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp509I TspRI TthHB8I