Restriction map for
pF1KB8681
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI AlwI AocI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoRV FbaI FseI HincII HindII
HindIII HpaI KasI MaeI MamI
MfeI MflI MluI MroI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1085 GGTACC G^GTACC
AcyI 774 GGCGTC G[GA]^CG[CT]C
AflIII 325 ACGTGT A^C[GA][CT]GT
Alw44I 727 GTGCAC G^TGCAC
Aor51HI 263 AGCGCT AGC^GCT
ApaLI 727 GTGCAC G^TGCAC
Asp718I 1085 GGTACC G^GTACC
AspHI 727 GTGCAC G[AT]GC[AT]^C
AvaI 132 CCCGAG C^[CT]CG[GA]G
AviII 404 TGCGCA TGC^GCA
BalI 830 TGGCCA TGG^CCA
BcgI 360 CGACTTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 266 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 266 GCTCAGC GC^T[ACGT]AGC
BsaHI 774 GGCGTC G[GA]^CG[CT]C
BsgI 845 GTGCAG GTGCAG(16/14)
BsiHKAI 727 GTGCAC G[AT]GC[AT]^C
BsmFI 329 GTCCC GGGAC(10/14)
BsmI 1045 GCATTC GAATGC(1/-1)
BsoBI 132 CCCGAG C^[CT]CG[GA]G
BsrFI 962 ACCGGC [GA]^CCGG[CT]
BssHII 240 GCGCGC G^CGCGC
BstEII 603 GGTGACC G^GT[ACGT]ACC
BstPI 603 GGTGACC G^GT[ACGT]ACC
CelII 266 GCTCAGC GC^T[ACGT]AGC
Cfr10I 962 ACCGGC [GA]^CCGG[CT]
EarI 547 GAAGAG CTCTTC(1/4)
Eco47III 263 AGCGCT AGC^GCT
EcoNI 918 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 603 GGTGACC G^GT[ACGT]ACC
EcoT14I 50 CCTTGG C^C[AT][AT]GG
EcoT22I 1043 ATGCAT ATGCA^T
FspI 404 TGCGCA TGC^GCA
HgaI 775 GCGTC GACGC(5/10)
Hin1I 774 GGCGTC G[GA]^CG[CT]C
KpnI 1085 GGTACC GGTAC^C
Ksp632I 547 GAAGAG CTCTTC(1/4)
KspI 166 CCGCGG CCGC^GG
MaeIII 604 GTGAC ^GT[ACGT]AC
MboII 547 GAAGA GAAGA(8/7)
MluNI 830 TGGCCA TGG^CCA
MscI 830 TGGCCA TGG^CCA
MslI 447 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 1043 ATGCAT ATGCA^T
PflMI 381 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 301 CAGCTG CAG^CTG
SacII 166 CCGCGG CCGC^GG
ScaI 623 AGTACT AGT^ACT
StuI 1115 AGGCCT AGG^CCT
StyI 50 CCTTGG C^C[AT][AT]GG
TfiI 990 GAATC G^A[AT]TC
Tsp45I 604 GTGAC ^GT[GC]AC
TspRI 706 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 381 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 79 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
538 CAGCCCCTG
BcnI 6 CCCGG CC^[GC]GG
102 CCGGG
BglI 101 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
571 GCCATGAAGGC
BpmI 895 CTCCAG CTGGAG(16/14)
57 CTGGAG
BspMI 236 ACCTGC ACCTGC(4/8)
1088 ACCTGC
BstXI 446 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
833 CCAAGTACATGG
DdeI 267 CTCAG C^T[ACGT]AG
413 CTGAG
DraII 248 AGGACCT [GA]G^G[ACGT]CC[CT]
914 AGGACCT
DrdI 490 GACTCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
784 GACTACATTGTC
EaeI 830 TGGCCA [CT]^GGCC[GA]
851 CGGCCA
EcoO109I 248 AGGACCT [GA]G^G[ACGT]CC[CT]
914 AGGACCT
MaeII 325 ACGT A^CGT
905 ACGT
NciI 6 CCCGG CC^[GC]GG
102 CCGGG
PleI 490 GACTC GAGTC(4/5)
875 GACTC
PpuMI 248 AGGACCT [GA]G^G[AT]CC[CT]
914 AGGACCT
SexAI 380 ACCAGGT A^CC[AT]GGT
524 ACCTGGT
SfaNI 1042 GATGC GCATC(5/9)
1059 GATGC
SmlI 514 CTCAAG C^T[CT][GA]AG
877 CTCAAG
TaqI 359 TCGA T^CGA
1049 TCGA
TthHB8I 359 TCGA T^CGA
1049 TCGA
XcmI 654 CCAGCAAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
761 CCACCGAGGCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanI BanII BbvI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII DsaI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MboI MnlI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfcI TseI