Restriction map for
pF1KB8683
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspI
AviII AvrII BalI BamHI BbeI
BcgI BcnI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BsgI
BsiWI BsmBI BspDI BspEI BspLU11I
BspMI BsrDI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 933 GGTACC G^GTACC
AccII 96 CGCG CG^CG
AflII 441 CTTAAG C^TTAAG
AflIII 1142 ACACGT A^C[GA][CT]GT
AhdI 1354 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 565 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 395 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 933 GGTACC G^GTACC
AspEI 1354 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 577 GAGCTC G[AT]GC[AT]^C
AvaI 1594 CCCGAG C^[CT]CG[GA]G
BbrPI 1143 CACGTG CAC^GTG
BciVI 696 GGATAC GTATCC(6/5)
BclI 629 TGATCA T^GATCA
BfaI 1801 CTAG C^TAG
BfrI 441 CTTAAG C^TTAAG
BglI 1237 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 19 GAGACC GGTCTC(1/5)
BsaWI 752 ACCGGA [AT]^CCGG[AT]
BsiEI 1209 CGGCCG CG[GA][CT]^CG
BsiHKAI 577 GAGCTC G[AT]GC[AT]^C
BsoBI 1594 CCCGAG C^[CT]CG[GA]G
Bsp1407I 272 TGTACA T^GTACA
BspHI 674 TCATGA T^CATGA
BsrGI 272 TGTACA T^GTACA
BssSI 1201 CACGAG CACGAG(-5/-1)
BstUI 96 CGCG CG^CG
DsaI 278 CCATGG C^C[GA][CT]GG
EagI 1209 CGGCCG C^GGCCG
Eam1105I 1354 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1209 CGGCCG C^GGCCG
Eco52I 1209 CGGCCG C^GGCCG
FbaI 629 TGATCA T^GATCA
HapII 753 CCGG C^CGG
HpaII 753 CCGG C^CGG
HphI 1605 GGTGA GGTGA(8/7)
KpnI 933 GGTACC GGTAC^C
MaeI 1801 CTAG C^TAG
MaeIII 227 GTGAC ^GT[ACGT]AC
MslI 840 CATTGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1212 CCGCTG C[AC]G^C[GT]G
MspI 753 CCGG C^CGG
MvnI 96 CGCG CG^CG
NcoI 278 CCATGG C^CATGG
PflMI 564 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1143 CACGTG CAC^GTG
PmlI 1143 CACGTG CAC^GTG
RcaI 674 TCATGA T^CATGA
SacI 577 GAGCTC GAGCT^C
SfiI 1236 GGCCTGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 272 TGTACA T^GTACA
TfiI 1503 GAATC G^A[AT]TC
Tsp45I 227 GTGAC ^GT[GC]AC
Van91I 564 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 395 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 194 GATCC GGATC(4/5)
1158 GATCC
BanI 637 GGCACC G^G[CT][GA]CC
933 GGTACC
BseRI 1282 GAGGAG GAGGAG(10/8)
1575 GAGGAG
BsrBI 61 GAGCGG CCGCTC(-3/-3)
745 GAGCGG
BstYI 193 AGATCC [GA]^GATC[CT]
1157 AGATCC
CfoI 97 GCGC GCG^C
1423 GCGC
EaeI 50 TGGCCG [CT]^GGCC[GA]
1209 CGGCCG
EcoRI 343 GAATTC G^AATTC
1876 GAATTC
HhaI 97 GCGC GCG^C
1423 GCGC
HinP1I 97 GCGC G^CGC
1423 GCGC
HindIII 1402 AAGCTT A^AGCTT
1529 AAGCTT
MflI 193 AGATCC [GA]^GATC[CT]
1157 AGATCC
MseI 410 TTAA T^TAA
442 TTAA
PstI 1018 CTGCAG CTGCA^G
1675 CTGCAG
SfcI 1018 CTGCAG C^T[GA][CT]AG
1675 CTGCAG
Tru9I 410 TTAA T^TAA
442 TTAA
XcmI 1316 CCAGTGTTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1721 CCAGAAGCGATTTGG
XhoII 193 AGATCC [GA]^GATC[CT]
1157 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BanII BbsI BbvCI BbvI
BmyI BpmI BpuAI BsaAI BsaJI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StuI
StyI TaqI TseI Tsp509I TspRI
TthHB8I