Restriction map for
pF1KB8684
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspHI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HapII HincII HindII
HpaI HpaII KasI KpnI KspI
MaeI MfeI MluNI MroI MscI
MslI MspA1I MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PleI PmeI PpuMI
PshAI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TthHB8I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccII 793 CGCG CG^CG
AclI 316 AACGTT AA^CGTT
AcyI 526 GACGCC G[GA]^CG[CT]C
AfaI 1017 GTAC GT^AC
AflIII 792 ACGCGT A^C[GA][CT]GT
AhdI 260 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 75 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 249 ATTAAT AT^TAAT
AsnI 249 ATTAAT AT^TAAT
Asp700I 379 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 260 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1117 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 390 GGACC G^G[AT]CC
BbrPI 142 CACGTG CAC^GTG
BbsI 895 GAAGAC GAAGAC(2/6)
BbvI 759 GCAGC GCAGC(8/12)
BpuAI 895 GAAGAC GAAGAC(2/6)
BsaAI 142 CACGTG [CT]AC^GT[GA]
BsaBI 1033 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 526 GACGCC G[GA]^CG[CT]C
BsaI 258 GAGACC GGTCTC(1/5)
BseRI 741 GAGGAG GAGGAG(10/8)
BsmAI 258 GAGAC GTCTC(1/5)
BsmI 85 GAATGC GAATGC(1/-1)
BsrDI 17 GCAATG GCAATG(2/0)
BstAPI 98 GCATTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 793 CGCG CG^CG
BstYI 1045 AGATCC [GA]^GATC[CT]
DraI 363 TTTAAA TTT^AAA
DraII 962 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 466 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 530 CCGTGG C^C[GA][CT]GG
EaeI 457 CGGCCA [CT]^GGCC[GA]
Eam1105I 260 GACCCAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 910 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 962 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 500 CCAAGG C^C[AT][AT]GG
Fnu4HI 759 GCAGC GC^[ACGT]GC
HaeII 772 AGCGCC [GA]GCGC^[CT]
HgaI 526 GACGC GACGC(5/10)
Hin1I 526 GACGCC G[GA]^CG[CT]C
ItaI 759 GCAGC GC^[ACGT]GC
MaeIII 1027 GTGAC ^GT[ACGT]AC
MamI 1033 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1045 AGATCC [GA]^GATC[CT]
MluI 792 ACGCGT A^CGCGT
MvnI 793 CGCG CG^CG
PflFI 1117 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 465 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 142 CACGTG CAC^GTG
PmlI 142 CACGTG CAC^GTG
PshBI 249 ATTAAT AT^TAAT
Psp1406I 316 AACGTT AA^CGTT
PstI 731 CTGCAG CTGCA^G
RsaI 1017 GTAC GT^AC
ScaI 1016 AGTACT AGT^ACT
SexAI 392 ACCAGGT A^CC[AT]GGT
SfcI 731 CTGCAG C^T[GA][CT]AG
StyI 500 CCAAGG C^C[AT][AT]GG
TseI 759 GCAGC G^C[AT]GC
Tsp45I 1027 GTGAC ^GT[GC]AC
Tth111I 1117 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 465 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1045 AGATCC [GA]^GATC[CT]
XmnI 379 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 337 AAATTT [GA]^AATT[CT]
922 AAATTC
AlwI 1046 GATCC GGATC(4/5)
450 GGATC
AocI 715 CCTGAGG CC^T[ACGT]AGG
877 CCTGAGG
ApoI 337 AAATTT [GA]^AATT[CT]
922 AAATTC
BsmFI 675 GGGAC GGGAC(10/14)
712 GTCCC
BstF5I 874 CATCC GGATG(2/0)
350 GGATG
Bsu36I 715 CCTGAGG CC^T[ACGT]AGG
877 CCTGAGG
Cac8I 222 GCAGGC GC[ACGT]^[ACGT]GC
790 GCACGC
Cfr13I 390 GGACC G^G[ACGT]CC
963 GGCCC
Eco81I 715 CCTGAGG CC^T[ACGT]AGG
877 CCTGAGG
EcoT22I 20 ATGCAT ATGCA^T
150 ATGCAT
FokI 874 CATCC GGATG(9/13)
350 GGATG
HindIII 416 AAGCTT A^AGCTT
1126 AAGCTT
HphI 28 GGTGA GGTGA(8/7)
549 GGTGA
MaeII 143 ACGT A^CGT
317 ACGT
NsiI 20 ATGCAT ATGCA^T
150 ATGCAT
Sau96I 390 GGACC G^G[ACGT]CC
963 GGCCC
SfaNI 873 GCATC GCATC(5/9)
1038 GCATC
SmlI 642 CTCAAG C^T[CT][GA]AG
691 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI BsaJI BsiYI BslI
BsrI BssKI BstNI CfoI DdeI
DpnII EarI EcoRII HaeIII HhaI
HinP1I HinfI Ksp632I MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI Sau3AI ScrFI
TfiI Tru9I Tsp509I TspRI