Restriction map for
pF1KB8686
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I KasI KpnI KspI MfeI
MluI MroI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AflIII 803 ACGTGT A^C[GA][CT]GT
AlwI 788 GGATC GGATC(4/5)
AlwNI 37 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 412 ATTAAT AT^TAAT
AsnI 412 ATTAAT AT^TAAT
Asp700I 463 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 81 CCCGAG C^[CT]CG[GA]G
BalI 1193 TGGCCA TGG^CCA
BanII 129 GAGCTC G[GA]GC[CT]^C
BciVI 850 GGATAC GTATCC(6/5)
BcnI 108 CCGGG CC^[GC]GG
BsaBI 145 GATTCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 749 GTGCAG GTGCAG(16/14)
BsiEI 105 CGGCCG CG[GA][CT]^CG
BsmFI 79 GTCCC GGGAC(10/14)
BsoBI 81 CCCGAG C^[CT]CG[GA]G
BspMI 75 GCAGGT ACCTGC(4/8)
BsrDI 859 GCAATG GCAATG(2/0)
BstBI 147 TTCGAA TT^CGAA
BstF5I 605 GGATG GGATG(2/0)
BstYI 788 GGATCT [GA]^GATC[CT]
Cac8I 20 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 18 GCGC GCG^C
DraII 77 AGGTCCC [GA]G^G[ACGT]CC[CT]
EagI 105 CGGCCG C^GGCCG
EclXI 105 CGGCCG C^GGCCG
Eco52I 105 CGGCCG C^GGCCG
EcoO109I 77 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 846 CCTTGG C^C[AT][AT]GG
FokI 605 GGATG GGATG(9/13)
HaeII 17 AGCGCC [GA]GCGC^[CT]
HapII 108 CCGG C^CGG
HhaI 18 GCGC GCG^C
HinP1I 18 GCGC G^CGC
HincII 1152 GTTAAC GT[CT]^[GA]AC
HindII 1152 GTTAAC GT[CT]^[GA]AC
HindIII 1176 AAGCTT A^AGCTT
HpaI 1152 GTTAAC GTT^AAC
HpaII 108 CCGG C^CGG
HphI 1171 GGTGA GGTGA(8/7)
MaeII 803 ACGT A^CGT
MamI 145 GATTCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 788 GGATCT [GA]^GATC[CT]
MluNI 1193 TGGCCA TGG^CCA
MscI 1193 TGGCCA TGG^CCA
MspA1I 28 CAGCGG C[AC]G^C[GT]G
MspI 108 CCGG C^CGG
NciI 108 CCGGG CC^[GC]GG
NspV 147 TTCGAA TT^CGAA
PflMI 36 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 77 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 412 ATTAAT AT^TAAT
SacI 129 GAGCTC GAGCT^C
SfaNI 116 GCATC GCATC(5/9)
SfuI 147 TTCGAA TT^CGAA
SspI 712 AATATT AAT^ATT
StyI 846 CCTTGG C^C[AT][AT]GG
Van91I 36 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 767 CCACTATGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 788 GGATCT [GA]^GATC[CT]
XmnI 463 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 822 AAATTT [GA]^AATT[CT]
1065 AAATTT
ApoI 822 AAATTT [GA]^AATT[CT]
1065 AAATTT
AspHI 129 GAGCTC G[AT]GC[AT]^C
686 GTGCTC
BbsI 1183 GAAGAC GAAGAC(2/6)
758 GTCTTC
BfaI 328 CTAG C^TAG
529 CTAG
BmyI 129 GAGCTC G[AGT]GC[ACT]^C
686 GTGCTC
BpmI 43 CTGGAG CTGGAG(16/14)
662 CTGGAG
BpuAI 1183 GAAGAC GAAGAC(2/6)
758 GTCTTC
BseRI 66 GAGGAG GAGGAG(10/8)
84 GAGGAG
BsiHKAI 129 GAGCTC G[AT]GC[AT]^C
686 GTGCTC
Bsp1286I 129 GAGCTC G[AGT]GC[ACT]^C
686 GTGCTC
BsrBI 46 GAGCGG CCGCTC(-3/-3)
87 GAGCGG
BsrI 755 CCAGT ACTGG(1/-1)
1078 CCAGT
DpnII 526 GATC ^GATC
789 GATC
EaeI 105 CGGCCG [CT]^GGCC[GA]
1193 TGGCCA
FauI 30 GCGGG CCCGC(4/6)
48 GCGGG
HaeIII 106 GGCC GG^CC
1194 GGCC
MaeI 328 CTAG C^TAG
529 CTAG
MaeIII 259 GTGAC ^GT[ACGT]AC
1144 GTCAC
MboI 526 GATC ^GATC
789 GATC
NdeII 526 GATC ^GATC
789 GATC
PstI 22 CTGCAG CTGCA^G
73 CTGCAG
Sau3AI 526 GATC ^GATC
789 GATC
Tsp45I 259 GTGAC ^GT[GC]AC
1144 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BsaJI BsiYI BslI BssKI BstNI
Cfr13I DdeI EarI EcoRII Fnu4HI
HinfI ItaI Ksp632I MboII MnlI
MseI MvaI MwoI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I