Restriction map for
pF1KB8687
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspBI
SwaI Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 240 CGCG CG^CG
AcyI 733 GACGCC G[GA]^CG[CT]C
AflIII 1671 ACATGT A^C[GA][CT]GT
AhdI 1649 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 113 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1807 ATTAAT AT^TAAT
AsnI 1807 ATTAAT AT^TAAT
AspEI 1649 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 17 GAGCTC G[AT]GC[AT]^C
BanI 157 GGCACC G^G[CT][GA]CC
BanII 17 GAGCTC G[GA]GC[CT]^C
BbsI 1474 GTCTTC GAAGAC(2/6)
BbvI 347 GCAGC GCAGC(8/12)
BglII 840 AGATCT A^GATCT
BmyI 17 GAGCTC G[AGT]GC[ACT]^C
BpmI 150 CTGGAG CTGGAG(16/14)
BpuAI 1474 GTCTTC GAAGAC(2/6)
BsaBI 514 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 733 GACGCC G[GA]^CG[CT]C
BsgI 720 CTGCAC GTGCAG(16/14)
BsiEI 249 CGGTCG CG[GA][CT]^CG
BsiHKAI 17 GAGCTC G[AT]GC[AT]^C
BsmAI 1545 GTCTC GTCTC(1/5)
Bsp1286I 17 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 1671 ACATGT A^CATGT
BsrDI 355 GCAATG GCAATG(2/0)
BsrFI 90 GCCGGC [GA]^CCGG[CT]
BssSI 193 CACGAG CACGAG(-5/-1)
BstNI 895 CCTGG CC^[AT]GG
BstUI 240 CGCG CG^CG
BstXI 228 CCACTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 90 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 90 GCCGGC [GA]^CCGG[CT]
DraI 1597 TTTAAA TTT^AAA
Eam1105I 1649 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 895 CCTGG ^CC[AT]GG
EcoRV 1099 GATATC GAT^ATC
EcoT14I 23 CCAAGG C^C[AT][AT]GG
HaeII 75 AGCGCC [GA]GCGC^[CT]
HgaI 733 GACGC GACGC(5/10)
Hin1I 733 GACGCC G[GA]^CG[CT]C
MamI 514 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 809 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 895 CCTGG CC^[AT]GG
MvnI 240 CGCG CG^CG
NaeI 90 GCCGGC GCC^GGC
NgoMIV 90 GCCGGC G^CCGGC
PpuMI 114 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1807 ATTAAT AT^TAAT
SacI 17 GAGCTC GAGCT^C
SfcI 1441 CTACAG C^T[GA][CT]AG
SmlI 1919 CTCAAG C^T[CT][GA]AG
SnaBI 1711 TACGTA TAC^GTA
StuI 26 AGGCCT AGG^CCT
StyI 23 CCAAGG C^C[AT][AT]GG
TseI 347 GCAGC G^C[AT]GC
XbaI 1642 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 338 AAATTT [GA]^AATT[CT]
657 AAATTT
AocI 118 CCTGAGG CC^T[ACGT]AGG
751 CCTGAGG
ApoI 338 AAATTT [GA]^AATT[CT]
657 AAATTT
AspI 63 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1462 GACAAAGTC
AvaI 71 CCCGAG C^[CT]CG[GA]G
974 CTCGGG
AvaII 115 GGTCC G^G[AT]CC
912 GGTCC
BsaAI 33 TACGTG [CT]AC^GT[GA]
1711 TACGTA
BsiYI 29 CCTCTACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
451 CCTTCTGTTGG
BslI 29 CCTCTACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
451 CCTTCTGTTGG
BsmFI 3 GTCCC GGGAC(10/14)
69 GTCCC
BsmI 424 GAATGC GAATGC(1/-1)
442 GCATTC
BsoBI 71 CCCGAG C^[CT]CG[GA]G
974 CTCGGG
BspMI 718 ACCTGC ACCTGC(4/8)
778 GCAGGT
BstYI 840 AGATCT [GA]^GATC[CT]
1359 GGATCT
Bsu36I 118 CCTGAGG CC^T[ACGT]AGG
751 CCTGAGG
DraII 114 AGGTCCT [GA]G^G[ACGT]CC[CT]
166 AGGCCCC
EaeI 182 TGGCCG [CT]^GGCC[GA]
502 CGGCCA
Eco81I 118 CCTGAGG CC^T[ACGT]AGG
751 CCTGAGG
EcoO109I 114 AGGTCCT [GA]G^G[ACGT]CC[CT]
166 AGGCCCC
MflI 840 AGATCT [GA]^GATC[CT]
1359 GGATCT
MspA1I 185 CCGCTG C[AC]G^C[GT]G
205 CCGCTG
NlaIV 157 GGCACC GG[ACGT]^[ACGT]CC
167 GGCCCC
PflFI 63 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1462 GACAAAGTC
TfiI 105 GAATC G^A[AT]TC
325 GAATC
Tth111I 63 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1462 GACAAAGTC
XcmI 447 CCAACCTTCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
505 CCATTGATAGATTGG
XhoII 840 AGATCT [GA]^GATC[CT]
1359 GGATCT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI BcnI
BfaI BsaJI BseRI BsrI BssKI
BstF5I CfoI Cfr13I DdeI DpnII
DsaI EarI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspI
MwoI NciI NdeII NlaIII NspI
PleI RsaI SapI Sau3AI Sau96I
ScrFI SspI TaqI Tru9I Tsp45I
Tsp509I TspRI TthHB8I