Restriction map for
pF1KB8688
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI KspI MboI
MfeI MflI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 104 GTCTAC GT^[AC][GT]AC
Alw44I 1044 GTGCAC G^TGCAC
AlwNI 1156 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1044 GTGCAC G^TGCAC
Asp700I 1305 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 934 GAGCTC G[GA]GC[CT]^C
BbvI 372 GCAGC GCAGC(8/12)
BfaI 667 CTAG C^TAG
BpmI 505 CTGGAG CTGGAG(16/14)
BsaBI 457 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 634 GAGGAG GAGGAG(10/8)
BsgI 20 GTGCAG GTGCAG(16/14)
BsiWI 1214 CGTACG C^GTACG
BsmBI 1189 GAGACG CGTCTC(1/5)
BsrDI 881 GCAATG GCAATG(2/0)
BstAPI 866 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1083 GGTCACC G^GT[ACGT]ACC
BstPI 1083 GGTCACC G^GT[ACGT]ACC
DraII 638 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1079 CCACGG C^C[GA][CT]GG
EcoO109I 638 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1083 GGTCACC G^GT[ACGT]ACC
EcoT14I 650 CCTTGG C^C[AT][AT]GG
FauI 228 GCGGG CCCGC(4/6)
HincII 1198 GTCAAC GT[CT]^[GA]AC
HindII 1198 GTCAAC GT[CT]^[GA]AC
MaeI 667 CTAG C^TAG
MaeII 889 ACGT A^CGT
MamI 457 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1128 CAGCTG C[AC]G^C[GT]G
NspI 1121 ACATGC [GA]CATG^[CT]
PpuMI 638 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1191 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1128 CAGCTG CAG^CTG
SacI 934 GAGCTC GAGCT^C
SapI 532 GAAGAGC GCTCTTC(1/4)
StuI 1139 AGGCCT AGG^CCT
StyI 650 CCTTGG C^C[AT][AT]GG
TseI 372 GCAGC G^C[AT]GC
XmnI 1305 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaI 398 CTCGGG C^[CT]CG[GA]G
1490 CTCGGG
BglI 284 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
841 GCCAAGCTGGC
BsmAI 74 GAGAC GTCTC(1/5)
1189 GAGAC
BsoBI 398 CTCGGG C^[CT]CG[GA]G
1490 CTCGGG
BspMI 771 ACCTGC ACCTGC(4/8)
138 GCAGGT
BsrBI 226 GAGCGG CCGCTC(-3/-3)
1174 GAGCGG
EcoRI 270 GAATTC G^AATTC
897 GAATTC
Fnu4HI 372 GCAGC GC^[ACGT]GC
1176 GCGGC
HaeIII 283 GGCC GG^CC
1140 GGCC
HphI 1060 TCACC GGTGA(8/7)
1085 TCACC
ItaI 372 GCAGC GC^[ACGT]GC
1176 GCGGC
MslI 987 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1450 CACAACAATG
PflMI 185 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1066 CCAAATACTGG
PleI 318 GACTC GAGTC(4/5)
814 GACTC
PstI 773 CTGCAG CTGCA^G
1153 CTGCAG
SfcI 773 CTGCAG C^T[GA][CT]AG
1153 CTGCAG
SspI 544 AATATT AAT^ATT
1255 AATATT
TfiI 1054 GAATC G^A[AT]TC
1266 GATTC
Van91I 185 CCATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1066 CCAAATACTGG
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BbsI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EarI EcoRII
FokI HinfI Ksp632I MaeIII MboII
MnlI MseI MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
TaqI Tru9I Tsp45I Tsp509I TspRI
TthHB8I