Restriction map for
pF1KB8690
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaAI BsaBI BsaI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI Eco47III EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HindIII HpaI KpnI MaeI
MamI MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Van91I XbaI XcmI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AhdI 1698 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1454 GTGCAC G^TGCAC
ApaLI 1454 GTGCAC G^TGCAC
AspEI 1698 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 749 TGGCCA TGG^CCA
BbeI 1068 GGCGCC GGCGC^C
BbsI 1764 GTCTTC GAAGAC(2/6)
BbvCI 1645 CCTCAGC CCTCAGC(-5/-2)
BglI 110 GCCTGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1764 GTCTTC GAAGAC(2/6)
BsaWI 1149 ACCGGA [AT]^CCGG[AT]
BsiEI 104 CGGCCG CG[GA][CT]^CG
BsmBI 1203 GAGACG CGTCTC(1/5)
BssHII 92 GCGCGC G^CGCGC
BstAPI 690 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1223 GGTCACC G^GT[ACGT]ACC
BstPI 1223 GGTCACC G^GT[ACGT]ACC
BstXI 881 CCATCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 842 AGATCC [GA]^GATC[CT]
DraIII 800 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 104 CGGCCG C^GGCCG
Eam1105I 1698 GACAGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 160 CTCTTC CTCTTC(1/4)
EclXI 104 CGGCCG C^GGCCG
Eco52I 104 CGGCCG C^GGCCG
EcoNI 1658 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1223 GGTCACC G^GT[ACGT]ACC
FseI 105 GGCCGGCC GGCCGG^CC
KasI 1068 GGCGCC G^GCGCC
Ksp632I 160 CTCTTC CTCTTC(1/4)
MaeII 742 ACGT A^CGT
MflI 842 AGATCC [GA]^GATC[CT]
MluNI 749 TGGCCA TGG^CCA
MscI 749 TGGCCA TGG^CCA
MslI 882 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 106 GCCGGC GCC^GGC
NarI 1068 GGCGCC GG^CGCC
NgoMIV 106 GCCGGC G^CCGGC
NlaIII 1446 CATG CATG^
PshAI 910 GACTACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 793 GAGCTC GAGCT^C
SapI 159 GCTCTTC GCTCTTC(1/4)
SfaNI 1324 GATGC GCATC(5/9)
SfiI 109 GGCCTGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1068 GGCGCC GGC^GCC
XhoII 842 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 372 GTAC GT^AC
735 GTAC
AlwI 843 GATCC GGATC(4/5)
1829 GGATC
ApaI 117 GGGCCC GGGCC^C
959 GGGCCC
AspI 633 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1758 GACTTTGTC
BlpI 1302 GCTGAGC GC^T[ACGT]AGC
1607 GCTCAGC
Bpu1102I 1302 GCTGAGC GC^T[ACGT]AGC
1607 GCTCAGC
BspMI 695 ACCTGC ACCTGC(4/8)
999 GCAGGT
BsrI 920 ACTGG ACTGG(1/-1)
1808 CCAGT
CelII 1302 GCTGAGC GC^T[ACGT]AGC
1607 GCTCAGC
KspI 583 CCGCGG CCGC^GG
1254 CCGCGG
MboII 161 TCTTC GAAGA(8/7)
1765 TCTTC
PflFI 633 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1758 GACTTTGTC
PpuMI 454 GGGTCCC [GA]G^G[AT]CC[CT]
1727 GGGACCC
PstI 688 CTGCAG CTGCA^G
1576 CTGCAG
PvuII 1047 CAGCTG CAG^CTG
1291 CAGCTG
RsaI 372 GTAC GT^AC
735 GTAC
SacII 583 CCGCGG CCGC^GG
1254 CCGCGG
SfcI 688 CTGCAG C^T[GA][CT]AG
1576 CTGCAG
Tth111I 633 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1758 GACTTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AocI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BssKI BstF5I
BstNI BstUI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIV PaeR7I PleI Sau3AI Sau96I
ScrFI SmaI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoI
XmaCI XmaI