Restriction map for
pF1KB8691
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BbrPI BbvCI
BcgI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BseAI BsiEI
BsiHKAI BsiWI BspEI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspI SwaI Tth111I
Van91I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 824 GAATTT [GA]^AATT[CT]
AocI 361 CCTGAGG CC^T[ACGT]AGG
ApoI 824 GAATTT [GA]^AATT[CT]
BamHI 248 GGATCC G^GATCC
BsaBI 1869 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2074 ACCGGA [AT]^CCGG[AT]
BsgI 1529 GTGCAG GTGCAG(16/14)
BsmBI 715 CGTCTC CGTCTC(1/5)
BsmI 805 GCATTC GAATGC(1/-1)
Bsp1407I 402 TGTACA T^GTACA
BspDI 388 ATCGAT AT^CGAT
BspHI 1341 TCATGA T^CATGA
BspMI 882 GCAGGT ACCTGC(4/8)
BsrDI 1763 GCAATG GCAATG(2/0)
BsrFI 998 GCCGGC [GA]^CCGG[CT]
BsrGI 402 TGTACA T^GTACA
Bsu36I 361 CCTGAGG CC^T[ACGT]AGG
Cfr10I 998 GCCGGC [GA]^CCGG[CT]
ClaI 388 ATCGAT AT^CGAT
Eco81I 361 CCTGAGG CC^T[ACGT]AGG
EcoNI 1116 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HpaI 1165 GTTAAC GTT^AAC
MamI 1869 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1166 TTAA T^TAA
NaeI 998 GCCGGC GCC^GGC
NgoMIV 998 GCCGGC G^CCGGC
PaeR7I 235 CTCGAG C^TCGAG
RcaI 1341 TCATGA T^CATGA
SapI 486 GAAGAGC GCTCTTC(1/4)
SbfI 645 CCTGCAGG CCTGCA^GG
ScaI 1811 AGTACT AGT^ACT
Sse8387I 645 CCTGCAGG CCTGCA^GG
SspBI 402 TGTACA T^GTACA
StuI 365 AGGCCT AGG^CCT
Tru9I 1166 TTAA T^TAA
XcmI 332 CCACGGAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 235 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 198 GGGCCC GGGCC^C
431 GGGCCC
BbeI 321 GGCGCC GGCGC^C
462 GGCGCC
BclI 836 TGATCA T^GATCA
1265 TGATCA
BfaI 436 CTAG C^TAG
2017 CTAG
BglII 1316 AGATCT A^GATCT
1352 AGATCT
EaeI 26 CGGCCA [CT]^GGCC[GA]
173 TGGCCG
EcoT14I 908 CCATGG C^C[AT][AT]GG
1653 CCATGG
FbaI 836 TGATCA T^GATCA
1265 TGATCA
HgaI 1276 GACGC GACGC(5/10)
714 GCGTC
HincII 478 GTTGAC GT[CT]^[GA]AC
1165 GTTAAC
HindII 478 GTTGAC GT[CT]^[GA]AC
1165 GTTAAC
KasI 321 GGCGCC G^GCGCC
462 GGCGCC
MaeI 436 CTAG C^TAG
2017 CTAG
NarI 321 GGCGCC GG^CGCC
462 GGCGCC
NcoI 908 CCATGG C^CATGG
1653 CCATGG
PpuMI 1457 AGGTCCC [GA]G^G[AT]CC[CT]
2033 AGGTCCC
PstI 646 CTGCAG CTGCA^G
880 CTGCAG
PvuII 667 CAGCTG CAG^CTG
1095 CAGCTG
SfoI 321 GGCGCC GGC^GCC
462 GGCGCC
SmlI 235 CTCGAG C^T[CT][GA]AG
718 CTCAAG
StyI 908 CCATGG C^C[AT][AT]GG
1653 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII