Restriction map for
pF1KB8693
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
Acc65I AccI AccII AclI AflII
AgeI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BsrBI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
KasI KpnI KspI MaeI MamI
MfeI MluI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 526 GACGTC GACGT^C
AccIII 1589 TCCGGA T^CCGGA
AcsI 757 GAATTT [GA]^AATT[CT]
AcyI 526 GACGTC G[GA]^CG[CT]C
AhdI 380 GACGGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 73 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 70 CCTCAGG CC^T[ACGT]AGG
Aor51HI 710 AGCGCT AGC^GCT
ApoI 757 GAATTT [GA]^AATT[CT]
AspEI 380 GACGGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 545 GTGCTC G[AT]GC[AT]^C
BalI 246 TGGCCA TGG^CCA
BanI 1543 GGTGCC G^G[CT][GA]CC
BciVI 744 GTATCC GTATCC(6/5)
BsaHI 526 GACGTC G[GA]^CG[CT]C
BseAI 1589 TCCGGA T^CCGGA
BsiHKAI 545 GTGCTC G[AT]GC[AT]^C
BsmBI 1293 GAGACG CGTCTC(1/5)
BspEI 1589 TCCGGA T^CCGGA
BspLU11I 1130 ACATGT A^CATGT
BspMI 425 GCAGGT ACCTGC(4/8)
BsrDI 1604 GCAATG GCAATG(2/0)
BstBI 551 TTCGAA TT^CGAA
Bsu36I 70 CCTCAGG CC^T[ACGT]AGG
DraIII 262 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 380 GACGGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 710 AGCGCT AGC^GCT
Eco81I 70 CCTCAGG CC^T[ACGT]AGG
EcoNI 402 CCTGTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 238 GATATC GAT^ATC
HgaI 464 GACGC GACGC(5/10)
Hin1I 526 GACGTC G[GA]^CG[CT]C
HincII 952 GTTAAC GT[CT]^[GA]AC
HindII 952 GTTAAC GT[CT]^[GA]AC
HpaI 952 GTTAAC GTT^AAC
MluNI 246 TGGCCA TGG^CCA
MroI 1589 TCCGGA T^CCGGA
MscI 246 TGGCCA TGG^CCA
MslI 1281 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 4 GCCGGC GCC^GGC
NgoMIV 4 GCCGGC G^CCGGC
NspV 551 TTCGAA TT^CGAA
PpuMI 40 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1465 CTGCAG CTGCA^G
SapI 547 GCTCTTC GCTCTTC(1/4)
SfcI 1465 CTGCAG C^T[GA][CT]AG
SfuI 551 TTCGAA TT^CGAA
SmaI 1253 CCCGGG CCC^GGG
SphI 1286 GCATGC GCATG^C
TfiI 219 GATTC G^A[AT]TC
XmaCI 1253 CCCGGG C^CCGGG
XmaI 1253 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaI 11 CTCGGG C^[CT]CG[GA]G
1253 CCCGGG
BanII 799 GGGCTC G[GA]GC[CT]^C
1054 GGGCTC
BbsI 1224 GAAGAC GAAGAC(2/6)
1276 GAAGAC
BglI 433 GCCACACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1483 GCCTTCTTGGC
BlpI 1146 GCTCAGC GC^T[ACGT]AGC
1478 GCTCAGC
Bpu1102I 1146 GCTCAGC GC^T[ACGT]AGC
1478 GCTCAGC
BpuAI 1224 GAAGAC GAAGAC(2/6)
1276 GAAGAC
BsaWI 1540 TCCGGT [AT]^CCGG[AT]
1589 TCCGGA
BsgI 372 GTGCAG GTGCAG(16/14)
497 GTGCAG
BsoBI 11 CTCGGG C^[CT]CG[GA]G
1253 CCCGGG
BsrFI 4 GCCGGC [GA]^CCGG[CT]
145 ACCGGC
BstAPI 499 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1467 GCAGGAGGTGC
BstYI 903 GGATCT [GA]^GATC[CT]
1085 AGATCC
CelII 1146 GCTCAGC GC^T[ACGT]AGC
1478 GCTCAGC
Cfr10I 4 GCCGGC [GA]^CCGG[CT]
145 ACCGGC
DraII 40 GGGTCCT [GA]G^G[ACGT]CC[CT]
1553 GGGGCCT
EcoO109I 40 GGGTCCT [GA]G^G[ACGT]CC[CT]
1553 GGGGCCT
EcoT14I 128 CCAAGG C^C[AT][AT]GG
1323 CCTTGG
MflI 903 GGATCT [GA]^GATC[CT]
1085 AGATCC
MseI 809 TTAA T^TAA
953 TTAA
NspI 1130 ACATGT [GA]CATG^[CT]
1286 GCATGC
StyI 128 CCAAGG C^C[AT][AT]GG
1323 CCTTGG
Tru9I 809 TTAA T^TAA
953 TTAA
XhoII 903 GGATCT [GA]^GATC[CT]
1085 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I