Restriction map for
pF1KB8695
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AviII AvrII
BanII BbeI BbrPI BbsI BbvCI
BcgI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XcmI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 134 GTCTAC GT^[AC][GT]AC
AflII 1621 CTTAAG C^TTAAG
AflIII 1672 ACATGT A^C[GA][CT]GT
AocI 1678 CCTAAGG CC^T[ACGT]AGG
AvaI 1088 CCCGGG C^[CT]CG[GA]G
BalI 1753 TGGCCA TGG^CCA
BamHI 1302 GGATCC G^GATCC
BanI 1453 GGTGCC G^G[CT][GA]CC
BciVI 1780 GTATCC GTATCC(6/5)
BclI 990 TGATCA T^GATCA
BfrI 1621 CTTAAG C^TTAAG
BglII 739 AGATCT A^GATCT
BmyI 1788 GGGCAC G[AGT]GC[ACT]^C
BseRI 1021 GAGGAG GAGGAG(10/8)
BsgI 1640 CTGCAC GTGCAG(16/14)
BsoBI 1088 CCCGGG C^[CT]CG[GA]G
Bsp1286I 1788 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 1672 ACATGT A^CATGT
Bsu36I 1678 CCTAAGG CC^T[ACGT]AGG
DraII 1084 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 1753 TGGCCA [CT]^GGCC[GA]
Eco81I 1678 CCTAAGG CC^T[ACGT]AGG
EcoO109I 1084 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1328 GAATTC G^AATTC
FbaI 990 TGATCA T^GATCA
MfeI 1337 CAATTG C^AATTG
MluNI 1753 TGGCCA TGG^CCA
MscI 1753 TGGCCA TGG^CCA
MslI 294 CATTTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1999 CAGCTG C[AC]G^C[GT]G
MunI 1337 CAATTG C^AATTG
NcoI 228 CCATGG C^CATGG
NdeI 1486 CATATG CA^TATG
NspI 1672 ACATGT [GA]CATG^[CT]
PflMI 745 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1084 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1999 CAGCTG CAG^CTG
SapI 973 GAAGAGC GCTCTTC(1/4)
ScaI 316 AGTACT AGT^ACT
SfaNI 755 GCATC GCATC(5/9)
SmaI 1088 CCCGGG CCC^GGG
SmlI 1621 CTTAAG C^T[CT][GA]AG
SspI 2064 AATATT AAT^ATT
Van91I 745 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1088 CCCGGG C^CCGGG
XmaI 1088 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 746 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
779 CAGAATCTG
AvaII 176 GGACC G^G[AT]CC
1085 GGACC
Bsp1407I 786 TGTACA T^GTACA
891 TGTACA
BsrGI 786 TGTACA T^GTACA
891 TGTACA
Cac8I 77 GCAAGC GC[ACGT]^[ACGT]GC
978 GCCTGC
DsaI 228 CCATGG C^C[GA][CT]GG
881 CCACGG
EcoT22I 1586 ATGCAT ATGCA^T
1732 ATGCAT
HapII 690 CCGG C^CGG
1089 CCGG
HindIII 896 AAGCTT A^AGCTT
1836 AAGCTT
HpaII 690 CCGG C^CGG
1089 CCGG
HphI 1068 GGTGA GGTGA(8/7)
100 TCACC
MspI 690 CCGG C^CGG
1089 CCGG
NsiI 1586 ATGCAT ATGCA^T
1732 ATGCAT
SspBI 786 TGTACA T^GTACA
891 TGTACA
TaqI 1518 TCGA T^CGA
1980 TCGA
Tsp45I 482 GTGAC ^GT[GC]AC
524 GTCAC
TthHB8I 1518 TCGA T^CGA
1980 TCGA
XbaI 1170 TCTAGA T^CTAGA
1968 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI Asp700I BbvI BcnI
BfaI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PshBI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI StyI TfiI Tru9I TseI
Tsp509I TspRI XhoII XmnI