Restriction map for
pF1KB8697
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 612 GAATTC [GA]^AATT[CT]
AgeI 1295 ACCGGT A^CCGGT
ApoI 612 GAATTC [GA]^AATT[CT]
BalI 1473 TGGCCA TGG^CCA
BamHI 414 GGATCC G^GATCC
BanI 1061 GGCACC G^G[CT][GA]CC
BbvCI 1339 GCTGAGG CCTCAGC(-5/-2)
BlpI 222 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 222 GCTGAGC GC^T[ACGT]AGC
BsgI 778 GTGCAG GTGCAG(16/14)
BsrDI 1433 GCAATG GCAATG(2/0)
BstEII 816 GGTGACC G^GT[ACGT]ACC
BstPI 816 GGTGACC G^GT[ACGT]ACC
BstXI 494 CCAAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 222 GCTGAGC GC^T[ACGT]AGC
DraIII 299 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1473 TGGCCA [CT]^GGCC[GA]
EcoO65I 816 GGTGACC G^GT[ACGT]ACC
EcoRI 612 GAATTC G^AATTC
HaeII 1560 AGCGCC [GA]GCGC^[CT]
HindIII 718 AAGCTT A^AGCTT
HinfI 1102 GAGTC G^A[ACGT]TC
MaeII 517 ACGT A^CGT
MluNI 1473 TGGCCA TGG^CCA
MscI 1473 TGGCCA TGG^CCA
NaeI 309 GCCGGC GCC^GGC
NgoMIV 309 GCCGGC G^CCGGC
PflMI 1392 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1295 ACCGGT A^CCGGT
PleI 1102 GAGTC GAGTC(4/5)
SapI 1054 GAAGAGC GCTCTTC(1/4)
SbfI 1377 CCTGCAGG CCTGCA^GG
SmaI 788 CCCGGG CCC^GGG
Sse8387I 1377 CCTGCAGG CCTGCA^GG
Tsp509I 613 AATT ^AATT
Van91I 1392 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 788 CCCGGG C^CCGGG
XmaI 788 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 682 CGCG CG^CG
955 CGCG
AlwNI 1000 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1456 CAGGACCTG
AspI 445 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
970 GACAAAGTC
AvaI 79 CCCGAG C^[CT]CG[GA]G
788 CCCGGG
BbsI 238 GAAGAC GAAGAC(2/6)
976 GTCTTC
BpuAI 238 GAAGAC GAAGAC(2/6)
976 GTCTTC
BsoBI 79 CCCGAG C^[CT]CG[GA]G
788 CCCGGG
BspMI 677 ACCTGC ACCTGC(4/8)
1380 GCAGGT
BsrFI 309 GCCGGC [GA]^CCGG[CT]
1295 ACCGGT
BstUI 682 CGCG CG^CG
955 CGCG
BstYI 414 GGATCC [GA]^GATC[CT]
431 AGATCC
Cfr10I 309 GCCGGC [GA]^CCGG[CT]
1295 ACCGGT
EarI 427 GAAGAG CTCTTC(1/4)
1054 GAAGAG
EcoNI 1086 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1625 CCTCCGCCAGG
EcoT14I 407 CCAAGG C^C[AT][AT]GG
494 CCAAGG
Ksp632I 427 GAAGAG CTCTTC(1/4)
1054 GAAGAG
MflI 414 GGATCC [GA]^GATC[CT]
431 AGATCC
MvnI 682 CGCG CG^CG
955 CGCG
NlaIII 906 CATG CATG^
1290 CATG
PflFI 445 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
970 GACAAAGTC
SfiI 699 GGCCGTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1511 GGCCAGAGGGGCC
StyI 407 CCAAGG C^C[AT][AT]GG
494 CCAAGG
Tth111I 445 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
970 GACAAAGTC
XhoII 414 GGATCC [GA]^GATC[CT]
431 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaII BanII BbvI BcnI
BglI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIV PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TseI Tsp45I
TspRI TthHB8I