Restriction map for
pF1KB8698
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbsI
BcgI BciVI BclI BcnI BglI
BglII BlnI BpuAI BsaHI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
CfoI ClaI CpoI DraI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PleI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1611 GGTACC G^GTACC
AciI 1108 GCGG CCGC(-3/-1)
AcsI 1498 AAATTT [GA]^AATT[CT]
AflII 114 CTTAAG C^TTAAG
AgeI 1581 ACCGGT A^CCGGT
AlwNI 1042 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1498 AAATTT [GA]^AATT[CT]
AseI 918 ATTAAT AT^TAAT
AsnI 918 ATTAAT AT^TAAT
Asp718I 1611 GGTACC G^GTACC
BbrPI 413 CACGTG CAC^GTG
BfrI 114 CTTAAG C^TTAAG
BlpI 1627 GCTAAGC GC^T[ACGT]AGC
BmyI 673 GTGCCC G[AGT]GC[ACT]^C
BpmI 236 CTGGAG CTGGAG(16/14)
Bpu1102I 1627 GCTAAGC GC^T[ACGT]AGC
BsaAI 413 CACGTG [CT]AC^GT[GA]
BsaBI 1228 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1581 ACCGGT [AT]^CCGG[AT]
Bsp1286I 673 GTGCCC G[AGT]GC[ACT]^C
BsrBI 1106 GAGCGG CCGCTC(-3/-3)
BsrFI 1581 ACCGGT [GA]^CCGG[CT]
CelII 1627 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1581 ACCGGT [GA]^CCGG[CT]
DraII 996 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1021 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 996 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 1108 GCGGG CCCGC(4/6)
HapII 1582 CCGG C^CGG
HgaI 1704 GCGTC GACGC(5/10)
HpaII 1582 CCGG C^CGG
KpnI 1611 GGTACC GGTAC^C
MamI 1228 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1582 CCGG C^CGG
NspI 167 ACATGC [GA]CATG^[CT]
PinAI 1581 ACCGGT A^CCGGT
PmaCI 413 CACGTG CAC^GTG
PmlI 413 CACGTG CAC^GTG
PpuMI 996 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 918 ATTAAT AT^TAAT
ScaI 1318 AGTACT AGT^ACT
SspI 823 AATATT AAT^ATT
TaqI 67 TCGA T^CGA
TthHB8I 67 TCGA T^CGA
XcmI 761 CCAAGGGCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 412 ACACGT A^C[GA][CT]GT
1248 ACGTGT
AvaII 227 GGTCC G^G[AT]CC
997 GGACC
BanI 540 GGTGCC G^G[CT][GA]CC
1611 GGTACC
BbvCI 910 CCTCAGC CCTCAGC(-5/-2)
1000 CCTCAGC
BsgI 689 GTGCAG GTGCAG(16/14)
1027 GTGCAG
BstAPI 1041 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1173 GCATCCCGTGC
BstEII 592 GGTGACC G^GT[ACGT]ACC
1584 GGTTACC
BstPI 592 GGTGACC G^GT[ACGT]ACC
1584 GGTTACC
EarI 1390 CTCTTC CTCTTC(1/4)
1711 GAAGAG
EcoO65I 592 GGTGACC G^GT[ACGT]ACC
1584 GGTTACC
EcoT14I 210 CCTTGG C^C[AT][AT]GG
761 CCAAGG
HincII 320 GTCAAC GT[CT]^[GA]AC
359 GTCAAC
HindII 320 GTCAAC GT[CT]^[GA]AC
359 GTCAAC
Ksp632I 1390 CTCTTC CTCTTC(1/4)
1711 GAAGAG
MaeII 414 ACGT A^CGT
1248 ACGT
MslI 9 CACTAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1414 CATTTTTGTG
PflMI 187 CCATCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
349 CCATTTCTTGG
PstI 605 CTGCAG CTGCA^G
1048 CTGCAG
SmlI 114 CTTAAG C^T[CT][GA]AG
1604 CTCAAG
StyI 210 CCTTGG C^C[AT][AT]GG
761 CCAAGG
Tsp45I 593 GTGAC ^GT[GC]AC
610 GTGAC
Van91I 187 CCATCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
349 CCATTTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AfaI AluI AlwI BbvI BfaI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI
XhoII