Restriction map for
pF1KB8699
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccI AccIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT14I FbaI FseI FspI
HincII HindII HpaI KspI MamI
MfeI MluNI MroI MscI MunI
NcoI NdeI NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tth111I
Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 106 GGTACC G^GTACC
AclI 1341 AACGTT AA^CGTT
AfaI 107 GTAC GT^AC
AflII 598 CTTAAG C^TTAAG
AflIII 1012 ACGCGT A^C[GA][CT]GT
Alw44I 346 GTGCAC G^TGCAC
AlwI 15 GATCC GGATC(4/5)
AlwNI 1520 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1379 CCTTAGG CC^T[ACGT]AGG
ApaLI 346 GTGCAC G^TGCAC
Asp700I 1450 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 106 GGTACC G^GTACC
AspHI 346 GTGCAC G[AT]GC[AT]^C
AvaI 73 CCCGGG C^[CT]CG[GA]G
BfrI 598 CTTAAG C^TTAAG
BglI 277 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 238 CTGCAC GTGCAG(16/14)
BsiHKAI 346 GTGCAC G[AT]GC[AT]^C
BsoBI 73 CCCGGG C^[CT]CG[GA]G
BspHI 915 TCATGA T^CATGA
BspMI 62 ACCTGC ACCTGC(4/8)
BsrBI 18 CCGCTC CCGCTC(-3/-3)
BssSI 349 CACGAG CACGAG(-5/-1)
BstYI 14 AGATCC [GA]^GATC[CT]
Bsu36I 1379 CCTTAGG CC^T[ACGT]AGG
DraI 1345 TTTAAA TTT^AAA
DraII 59 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 284 TGGCCG [CT]^GGCC[GA]
Eco81I 1379 CCTTAGG CC^T[ACGT]AGG
EcoO109I 59 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 725 GAATTC G^AATTC
EcoT22I 740 ATGCAT ATGCA^T
FauI 331 CCCGC CCCGC(4/6)
HgaI 356 GCGTC GACGC(5/10)
KpnI 106 GGTACC GGTAC^C
MaeII 1342 ACGT A^CGT
MflI 14 AGATCC [GA]^GATC[CT]
MluI 1012 ACGCGT A^CGCGT
MslI 1129 CATCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 286 GCCGGC GCC^GGC
NgoMIV 286 GCCGGC G^CCGGC
NsiI 740 ATGCAT ATGCA^T
NspI 742 GCATGT [GA]CATG^[CT]
PleI 380 GACTC GAGTC(4/5)
PpuMI 59 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1341 AACGTT AA^CGTT
PvuII 184 CAGCTG CAG^CTG
RcaI 915 TCATGA T^CATGA
RsaI 107 GTAC GT^AC
SexAI 1085 ACCTGGT A^CC[AT]GGT
SfcI 181 CTACAG C^T[GA][CT]AG
SfiI 276 GGCCCTTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 73 CCCGGG CCC^GGG
SmlI 598 CTTAAG C^T[CT][GA]AG
XbaI 678 TCTAGA T^CTAGA
XhoII 14 AGATCC [GA]^GATC[CT]
XmaCI 73 CCCGGG C^CCGGG
XmaI 73 CCCGGG C^CCGGG
XmnI 1450 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 312 CGCG CG^CG
1013 CGCG
AcyI 220 GGCGCC G[GA]^CG[CT]C
855 GGCGCC
AvaII 33 GGACC G^G[AT]CC
60 GGACC
BanII 70 GAGCCC G[GA]GC[CT]^C
534 GAGCCC
BbeI 220 GGCGCC GGCGC^C
855 GGCGCC
BfaI 664 CTAG C^TAG
679 CTAG
BsaHI 220 GGCGCC G[GA]^CG[CT]C
855 GGCGCC
BsmAI 378 GAGAC GTCTC(1/5)
1230 GTCTC
BsrFI 176 ACCGGC [GA]^CCGG[CT]
286 GCCGGC
BstBI 1151 TTCGAA TT^CGAA
1220 TTCGAA
BstUI 312 CGCG CG^CG
1013 CGCG
CfoI 221 GCGC GCG^C
856 GCGC
Cfr10I 176 ACCGGC [GA]^CCGG[CT]
286 GCCGGC
DpnII 15 GATC ^GATC
1618 GATC
HaeII 220 GGCGCC [GA]GCGC^[CT]
855 GGCGCC
HhaI 221 GCGC GCG^C
856 GCGC
Hin1I 220 GGCGCC G[GA]^CG[CT]C
855 GGCGCC
HinP1I 221 GCGC G^CGC
856 GCGC
KasI 220 GGCGCC G^GCGCC
855 GGCGCC
MaeI 664 CTAG C^TAG
679 CTAG
MboI 15 GATC ^GATC
1618 GATC
MspA1I 184 CAGCTG C[AC]G^C[GT]G
235 CCGCTG
MvnI 312 CGCG CG^CG
1013 CGCG
NarI 220 GGCGCC GG^CGCC
855 GGCGCC
NdeII 15 GATC ^GATC
1618 GATC
NspV 1151 TTCGAA TT^CGAA
1220 TTCGAA
Sau3AI 15 GATC ^GATC
1618 GATC
SfaNI 739 GATGC GCATC(5/9)
850 GATGC
SfoI 220 GGCGCC GGC^GCC
855 GGCGCC
SfuI 1151 TTCGAA TT^CGAA
1220 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AluI ApoI BanI
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EarI
EcoRII Fnu4HI FokI HaeIII HapII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboII MnlI MseI
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I