Restriction map for
pF1KB8704
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbsI BciVI
BclI BfrI BglII BlnI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CfoI
Cfr13I ClaI CpoI DpnII DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaI KasI KpnI
KspI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI MvnI NarI
NcoI NdeI NdeII NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 110 AAATTC [GA]^AATT[CT]
AlwNI 275 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 110 AAATTC [GA]^AATT[CT]
BanI 250 GGCACC G^G[CT][GA]CC
BanII 298 GAGCCC G[GA]GC[CT]^C
BbvCI 184 GCTGAGG CCTCAGC(-5/-2)
BcgI 504 CGATGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 119 CTAG C^TAG
BglI 370 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 407 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 407 GCTCAGC GC^T[ACGT]AGC
BsrFI 403 GCCGGC [GA]^CCGG[CT]
BstAPI 550 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 375 CCAGG CC^[AT]GG
CelII 407 GCTCAGC GC^T[ACGT]AGC
Cfr10I 403 GCCGGC [GA]^CCGG[CT]
DsaI 126 CCGTGG C^C[GA][CT]GG
EcoRII 375 CCAGG ^CC[AT]GG
HindIII 434 AAGCTT A^AGCTT
HphI 346 GGTGA GGTGA(8/7)
MaeI 119 CTAG C^TAG
MaeIII 520 GTCAC ^GT[ACGT]AC
MspA1I 410 CAGCTG C[AC]G^C[GT]G
MvaI 375 CCAGG CC^[AT]GG
NaeI 403 GCCGGC GCC^GGC
NgoMIV 403 GCCGGC G^CCGGC
NheI 118 GCTAGC G^CTAGC
NlaIII 709 CATG CATG^
NspI 708 ACATGC [GA]CATG^[CT]
PflMI 274 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 410 CAGCTG CAG^CTG
SfcI 632 CTGTAG C^T[GA][CT]AG
Tsp45I 520 GTCAC ^GT[GC]AC
Tsp509I 111 AATT ^AATT
Van91I 274 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 101 CCGC CCGC(-3/-1)
548 CCGC
AocI 649 CCTAAGG CC^T[ACGT]AGG
691 CCTGAGG
BcnI 301 CCCGG CC^[GC]GG
662 CCCGG
BmyI 298 GAGCCC G[AGT]GC[ACT]^C
659 GTGCCC
BsiYI 274 CCAGCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
477 CCCAGCCAAGG
BslI 274 CCAGCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
477 CCCAGCCAAGG
BsmAI 415 GAGAC GTCTC(1/5)
592 GAGAC
Bsp1286I 298 GAGCCC G[AGT]GC[ACT]^C
659 GTGCCC
BsrI 280 ACTGG ACTGG(1/-1)
595 ACTGG
Bsu36I 649 CCTAAGG CC^T[ACGT]AGG
691 CCTGAGG
EarI 616 GAAGAG CTCTTC(1/4)
682 GAAGAG
Eco81I 649 CCTAAGG CC^T[ACGT]AGG
691 CCTGAGG
HaeIII 99 GGCC GG^CC
402 GGCC
Ksp632I 616 GAAGAG CTCTTC(1/4)
682 GAAGAG
NciI 301 CCCGG CC^[GC]GG
662 CCCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BbvI BsaJI BseRI BssKI
BstF5I Cac8I DdeI EcoT14I Fnu4HI
FokI HapII HpaII ItaI MboII
MnlI MspI MwoI NlaIV ScrFI
SfaNI StyI TseI TspRI