Restriction map for
pF1KB8706
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BcnI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmFI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HapII HgaI Hin1I HindIII HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccII 26 CGCG CG^CG
AfaI 623 GTAC GT^AC
AflII 823 CTTAAG C^TTAAG
AhdI 13 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 13 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1338 GGTCC G^G[AT]CC
AvrII 1383 CCTAGG C^CTAGG
BciVI 879 GTATCC GTATCC(6/5)
BfrI 823 CTTAAG C^TTAAG
BlnI 1383 CCTAGG C^CTAGG
BpmI 739 CTCCAG CTGGAG(16/14)
BsaJI 1383 CCTAGG C^C[ACGT][ACGT]GG
BsmBI 744 GAGACG CGTCTC(1/5)
Bsp1407I 622 TGTACA T^GTACA
BsrGI 622 TGTACA T^GTACA
BstUI 26 CGCG CG^CG
Eam1105I 13 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 1383 CCTAGG C^C[AT][AT]GG
HincII 166 GTCAAC GT[CT]^[GA]AC
HindII 166 GTCAAC GT[CT]^[GA]AC
MvnI 26 CGCG CG^CG
PshAI 447 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1355 CTGCAG CTGCA^G
PvuII 212 CAGCTG CAG^CTG
RsaI 623 GTAC GT^AC
SacI 1191 GAGCTC GAGCT^C
SmlI 823 CTTAAG C^T[CT][GA]AG
SspBI 622 TGTACA T^GTACA
SspI 943 AATATT AAT^ATT
StyI 1383 CCTAGG C^C[AT][AT]GG
Tsp45I 1321 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AspHI 189 GAGCAC G[AT]GC[AT]^C
1191 GAGCTC
BanII 431 GAGCCC G[GA]GC[CT]^C
1191 GAGCTC
BbsI 691 GAAGAC GAAGAC(2/6)
762 GTCTTC
BbvI 33 GCAGC GCAGC(8/12)
1042 GCTGC
BpuAI 691 GAAGAC GAAGAC(2/6)
762 GTCTTC
BsiHKAI 189 GAGCAC G[AT]GC[AT]^C
1191 GAGCTC
BsrDI 251 GCAATG GCAATG(2/0)
973 GCAATG
BsrI 1221 ACTGG ACTGG(1/-1)
628 CCAGT
BssKI 696 CCTGG ^CC[ACGT]GG
997 CCTGG
BstNI 696 CCTGG CC^[AT]GG
997 CCTGG
CfoI 25 GCGC GCG^C
1045 GCGC
DdeI 275 CTCAG C^T[ACGT]AG
723 CTTAG
DraII 993 GGGGCCT [GA]G^G[ACGT]CC[CT]
1379 AGGCCCT
EcoNI 414 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
997 CCTGGAATAGG
EcoO109I 993 GGGGCCT [GA]G^G[ACGT]CC[CT]
1379 AGGCCCT
EcoRII 696 CCTGG ^CC[AT]GG
997 CCTGG
HhaI 25 GCGC GCG^C
1045 GCGC
HinP1I 25 GCGC G^CGC
1045 GCGC
MseI 824 TTAA T^TAA
1265 TTAA
MslI 651 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1143 CACAACTATG
MspA1I 212 CAGCTG C[AC]G^C[GT]G
884 CCGCTG
MvaI 696 CCTGG CC^[AT]GG
997 CCTGG
NlaIV 304 GGCTCC GG[ACGT]^[ACGT]CC
993 GGGGCC
PleI 819 GACTC GAGTC(4/5)
58 GAGTC
ScrFI 696 CCTGG CC^[ACGT]GG
997 CCTGG
SfcI 1098 CTATAG C^T[GA][CT]AG
1355 CTGCAG
Tru9I 824 TTAA T^TAA
1265 TTAA
TseI 33 GCAGC G^C[AT]GC
1042 GCTGC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AcsI AluI ApoI BfaI
BmyI BsiYI BslI BsmAI Bsp1286I
BstF5I Cac8I Cfr13I DpnII FauI
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MwoI NdeII NlaIII Sau3AI
Sau96I SfaNI TaqI TfiI Tsp509I
TspRI TthHB8I