Restriction map for
pF1KB8707
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII BalI
BamHI BanI BanII BbeI BbvCI
BciVI BclI BcnI BglI BglII
BlpI Bpu1102I BsaBI BseAI BseRI
BsiEI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 1520 GTAGAC GT^[AC][GT]AC
AccII 25 CGCG CG^CG
AcyI 1319 GACGCC G[GA]^CG[CT]C
AflII 549 CTTAAG C^TTAAG
AlwI 1411 GATCC GGATC(4/5)
AlwNI 1466 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 400 CCTGAGG CC^T[ACGT]AGG
AspHI 1402 GTGCTC G[AT]GC[AT]^C
AvrII 865 CCTAGG C^CTAGG
BbrPI 1373 CACGTG CAC^GTG
BcgI 581 CGAAGTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 549 CTTAAG C^TTAAG
BlnI 865 CCTAGG C^CTAGG
BmyI 1402 GTGCTC G[AGT]GC[ACT]^C
BpmI 743 CTGGAG CTGGAG(16/14)
BsaAI 1373 CACGTG [CT]AC^GT[GA]
BsaHI 1319 GACGCC G[GA]^CG[CT]C
BsaI 1514 GGTCTC GGTCTC(1/5)
BsaWI 1189 TCCGGT [AT]^CCGG[AT]
BsgI 1039 GTGCAG GTGCAG(16/14)
BsiHKAI 1402 GTGCTC G[AT]GC[AT]^C
BsiWI 577 CGTACG C^GTACG
BsiYI 1372 CCACGTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1372 CCACGTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 1317 GAGACG CGTCTC(1/5)
BsmI 628 GAATGC GAATGC(1/-1)
Bsp1286I 1402 GTGCTC G[AGT]GC[ACT]^C
BspMI 1041 GCAGGT ACCTGC(4/8)
BstUI 25 CGCG CG^CG
BstXI 226 CCATGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1410 AGATCC [GA]^GATC[CT]
Bsu36I 400 CCTGAGG CC^T[ACGT]AGG
DraI 379 TTTAAA TTT^AAA
DraII 1173 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1373 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 400 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1173 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 865 CCTAGG C^C[AT][AT]GG
EcoT22I 559 ATGCAT ATGCA^T
HapII 1190 CCGG C^CGG
Hin1I 1319 GACGCC G[GA]^CG[CT]C
HpaII 1190 CCGG C^CGG
MaeII 1374 ACGT A^CGT
MflI 1410 AGATCC [GA]^GATC[CT]
MspA1I 1120 CAGCTG C[AC]G^C[GT]G
MspI 1190 CCGG C^CGG
MvnI 25 CGCG CG^CG
NsiI 559 ATGCAT ATGCA^T
PflMI 1372 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 76 GACTC GAGTC(4/5)
PmaCI 1373 CACGTG CAC^GTG
PmlI 1373 CACGTG CAC^GTG
PpuMI 1173 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1463 CTGCAG CTGCA^G
PvuII 1120 CAGCTG CAG^CTG
ScaI 1495 AGTACT AGT^ACT
SmlI 549 CTTAAG C^T[CT][GA]AG
SphI 466 GCATGC GCATG^C
StyI 865 CCTAGG C^C[AT][AT]GG
Van91I 1372 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1050 TCTAGA T^CTAGA
XhoII 1410 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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Asp700I 328 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1340 GAAGTGTTTC
AvaII 1174 GGACC G^G[AT]CC
1369 GGACC
BbsI 73 GAAGAC GAAGAC(2/6)
985 GAAGAC
BpuAI 73 GAAGAC GAAGAC(2/6)
985 GAAGAC
BsaJI 865 CCTAGG C^C[ACGT][ACGT]GG
1323 CCCAGG
BsmFI 959 GGGAC GGGAC(10/14)
1173 GGGAC
BspHI 69 TCATGA T^CATGA
657 TCATGA
BsrI 775 ACTGG ACTGG(1/-1)
518 CCAGT
HgaI 298 GACGC GACGC(5/10)
1319 GACGC
MslI 227 CATGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1300 CATCAGCGTG
NspI 466 GCATGC [GA]CATG^[CT]
557 ACATGC
RcaI 69 TCATGA T^CATGA
657 TCATGA
SfcI 1105 CTACAG C^T[GA][CT]AG
1463 CTGCAG
XcmI 219 CCAAGAGCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
226 CCATGTGGGTGGTGG
XmnI 328 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1340 GAAGTGTTTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BfaI BsmAI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I