Restriction map for
pF1KB8709
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI HinP1I HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI RsrII SacII SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AatII 1110 GACGTC GACGT^C
AccI 547 GTCGAC GT^[AC][GT]AC
AccII 735 CGCG CG^CG
AccIII 801 TCCGGA T^CCGGA
AclI 127 AACGTT AA^CGTT
ApaI 257 GGGCCC GGGCC^C
Asp700I 183 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 246 TGGCCA TGG^CCA
BamHI 69 GGATCC G^GATCC
BfaI 160 CTAG C^TAG
BsaAI 769 TACGTG [CT]AC^GT[GA]
BsaWI 801 TCCGGA [AT]^CCGG[AT]
BseAI 801 TCCGGA T^CCGGA
BspEI 801 TCCGGA T^CCGGA
BspMI 332 ACCTGC ACCTGC(4/8)
BstUI 735 CGCG CG^CG
DraIII 157 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 246 TGGCCA [CT]^GGCC[GA]
MaeI 160 CTAG C^TAG
MluNI 246 TGGCCA TGG^CCA
MroI 801 TCCGGA T^CCGGA
MscI 246 TGGCCA TGG^CCA
MseI 854 TTAA T^TAA
MvnI 735 CGCG CG^CG
Psp1406I 127 AACGTT AA^CGTT
SacI 739 GAGCTC GAGCT^C
SalI 547 GTCGAC G^TCGAC
SmlI 168 CTTGAG C^T[CT][GA]AG
StuI 870 AGGCCT AGG^CCT
Tru9I 854 TTAA T^TAA
XmnI 183 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 474 GAATTT [GA]^AATT[CT]
987 AAATTT
AcyI 1009 GACGCC G[GA]^CG[CT]C
1110 GACGTC
AfaI 15 GTAC GT^AC
897 GTAC
AlwNI 271 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
328 CAGCACCTG
ApoI 474 GAATTT [GA]^AATT[CT]
987 AAATTT
AspHI 711 GAGCAC G[AT]GC[AT]^C
739 GAGCTC
BanI 238 GGCACC G^G[CT][GA]CC
690 GGTGCC
BanII 257 GGGCCC G[GA]GC[CT]^C
739 GAGCTC
BbsI 23 GTCTTC GAAGAC(2/6)
787 GTCTTC
BcnI 108 CCGGG CC^[GC]GG
843 CCGGG
BpuAI 23 GTCTTC GAAGAC(2/6)
787 GTCTTC
BsaHI 1009 GACGCC G[GA]^CG[CT]C
1110 GACGTC
BsiHKAI 711 GAGCAC G[AT]GC[AT]^C
739 GAGCTC
BsmAI 304 GAGAC GTCTC(1/5)
673 GAGAC
Bsp1407I 14 TGTACA T^GTACA
896 TGTACA
BspHI 729 TCATGA T^CATGA
1154 TCATGA
BsrGI 14 TGTACA T^GTACA
896 TGTACA
BstEII 909 GGTCACC G^GT[ACGT]ACC
1095 GGTCACC
BstF5I 579 CATCC GGATG(2/0)
795 CATCC
BstPI 909 GGTCACC G^GT[ACGT]ACC
1095 GGTCACC
BstYI 69 GGATCC [GA]^GATC[CT]
311 AGATCC
DraII 110 GGGGCCT [GA]G^G[ACGT]CC[CT]
272 AGGGCCT
EcoO109I 110 GGGGCCT [GA]G^G[ACGT]CC[CT]
272 AGGGCCT
EcoO65I 909 GGTCACC G^GT[ACGT]ACC
1095 GGTCACC
FokI 579 CATCC GGATG(9/13)
795 CATCC
HgaI 733 GACGC GACGC(5/10)
1009 GACGC
Hin1I 1009 GACGCC G[GA]^CG[CT]C
1110 GACGTC
MflI 69 GGATCC [GA]^GATC[CT]
311 AGATCC
MwoI 239 GCACCTGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
872 GCCTGACCAGC
NciI 108 CCGGG CC^[GC]GG
843 CCGGG
PflMI 60 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
270 CCAGGGCCTGG
RcaI 729 TCATGA T^CATGA
1154 TCATGA
RsaI 15 GTAC GT^AC
897 GTAC
SfaNI 206 GCATC GCATC(5/9)
998 GCATC
SspBI 14 TGTACA T^GTACA
896 TGTACA
Tsp45I 910 GTCAC ^GT[GC]AC
1096 GTCAC
Van91I 60 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
270 CCAGGGCCTGG
XcmI 578 CCATCCTCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
878 CCAGCAGCCAGGTGG
XhoII 69 GGATCC [GA]^GATC[CT]
311 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BsrDI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI NcoI NdeII
NlaIII NlaIV PleI PvuII Sau3AI
Sau96I ScrFI StyI TaqI TseI
Tsp509I TspRI TthHB8I