Restriction map for
pF1KB8713
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AlwNI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI MaeII MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StyI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccII 34 CGCG CG^CG
Alw44I 489 GTGCAC G^TGCAC
AocI 850 CCTCAGG CC^T[ACGT]AGG
ApaLI 489 GTGCAC G^TGCAC
AvaI 410 CCCGAG C^[CT]CG[GA]G
AvaII 918 GGTCC G^G[AT]CC
BbsI 346 GTCTTC GAAGAC(2/6)
BfaI 706 CTAG C^TAG
BpuAI 346 GTCTTC GAAGAC(2/6)
BsaI 74 GGTCTC GGTCTC(1/5)
BseRI 906 CTCCTC GAGGAG(10/8)
BsoBI 410 CCCGAG C^[CT]CG[GA]G
BstF5I 45 CATCC GGATG(2/0)
BstUI 34 CGCG CG^CG
Bsu36I 850 CCTCAGG CC^T[ACGT]AGG
DraI 337 TTTAAA TTT^AAA
DsaI 33 CCGCGG C^C[GA][CT]GG
Eco81I 850 CCTCAGG CC^T[ACGT]AGG
FokI 45 CATCC GGATG(9/13)
KspI 33 CCGCGG CCGC^GG
MaeI 706 CTAG C^TAG
MspA1I 33 CCGCGG C[AC]G^C[GT]G
MvnI 34 CGCG CG^CG
NdeI 308 CATATG CA^TATG
SacI 88 GAGCTC GAGCT^C
SacII 33 CCGCGG CCGC^GG
StuI 847 AGGCCT AGG^CCT
XcmI 307 CCATATGGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 370 GAATTT [GA]^AATT[CT]
458 GAATTT
AlwI 202 GATCC GGATC(4/5)
835 GGATC
ApoI 370 GAATTT [GA]^AATT[CT]
458 GAATTT
AspHI 88 GAGCTC G[AT]GC[AT]^C
489 GTGCAC
BcnI 168 CCGGG CC^[GC]GG
559 CCCGG
BsiHKAI 88 GAGCTC G[AT]GC[AT]^C
489 GTGCAC
BsmAI 62 GTCTC GTCTC(1/5)
75 GTCTC
BstNI 845 CCAGG CC^[AT]GG
870 CCAGG
EarI 893 CTCTTC CTCTTC(1/4)
909 CTCTTC
EcoRII 845 CCAGG ^CC[AT]GG
870 CCAGG
HapII 168 CCGG C^CGG
560 CCGG
HinfI 9 GAATC G^A[ACGT]TC
856 GAATC
HpaII 168 CCGG C^CGG
560 CCGG
Ksp632I 893 CTCTTC CTCTTC(1/4)
909 CTCTTC
MspI 168 CCGG C^CGG
560 CCGG
MvaI 845 CCAGG CC^[AT]GG
870 CCAGG
NciI 168 CCGGG CC^[GC]GG
559 CCCGG
NlaIII 188 CATG CATG^
207 CATG
TfiI 9 GAATC G^A[AT]TC
856 GAATC
TspRI 198 CAGTG CA[GC]TG[ACGT][ACGT]^
249 CACTG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI BanII BbvI BciVI
BmyI BsaJI BsiYI BslI Bsp1286I
BspMI BsrI BssKI Cac8I Cfr13I
DdeI DpnII FauI Fnu4HI HaeIII
HphI ItaI MboI MboII MnlI
MseI MwoI NdeII NlaIV PflMI
PstI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI Sse8387I Tru9I TseI
Tsp509I Van91I