Restriction map for
pF1KB8714
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseRI
BsiEI BsiWI BsmBI BsmI BspDI
BspHI BspLU11I BspMI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MaeI MaeII MamI MfeI MluI
MslI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SbfI ScaI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI Tsp509I TthHB8I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 91 TCCGGA T^CCGGA
AspHI 108 GTGCTC G[AT]GC[AT]^C
AspI 586 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1002 TGGCCA TGG^CCA
BcgI 312 CGAGGAAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 91 TCCGGA T^CCGGA
BsgI 145 GTGCAG GTGCAG(16/14)
BsiHKAI 108 GTGCTC G[AT]GC[AT]^C
Bsp1407I 251 TGTACA T^GTACA
BspEI 91 TCCGGA T^CCGGA
BsrDI 830 CATTGC GCAATG(2/0)
BsrGI 251 TGTACA T^GTACA
BstEII 120 GGTGACC G^GT[ACGT]ACC
BstPI 120 GGTGACC G^GT[ACGT]ACC
BstYI 809 GGATCT [GA]^GATC[CT]
EarI 1107 GAAGAG CTCTTC(1/4)
EcoO65I 120 GGTGACC G^GT[ACGT]ACC
HgaI 95 GACGC GACGC(5/10)
Ksp632I 1107 GAAGAG CTCTTC(1/4)
KspI 10 CCGCGG CCGC^GG
MflI 809 GGATCT [GA]^GATC[CT]
MluNI 1002 TGGCCA TGG^CCA
MroI 91 TCCGGA T^CCGGA
MscI 1002 TGGCCA TGG^CCA
MseI 1174 TTAA T^TAA
NcoI 908 CCATGG C^CATGG
NspI 1278 ACATGC [GA]CATG^[CT]
PflFI 586 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 951 GAGTC GAGTC(4/5)
SacII 10 CCGCGG CCGC^GG
SapI 1107 GAAGAGC GCTCTTC(1/4)
SexAI 672 ACCAGGT A^CC[AT]GGT
SfiI 749 GGCCACCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 251 TGTACA T^GTACA
StuI 576 AGGCCT AGG^CCT
TfiI 995 GAATC G^A[AT]TC
Tru9I 1174 TTAA T^TAA
Tth111I 586 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 809 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 11 CGCG CG^CG
1141 CGCG
AlwI 809 GGATC GGATC(4/5)
1295 GGATC
AlwNI 99 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
782 CAGCCCCTG
AocI 517 CCTGAGG CC^T[ACGT]AGG
1087 CCTAAGG
BsaWI 91 TCCGGA [AT]^CCGG[AT]
1292 ACCGGA
BsrBI 532 CCGCTC CCGCTC(-3/-3)
439 GAGCGG
BstF5I 374 GGATG GGATG(2/0)
659 GGATG
BstUI 11 CGCG CG^CG
1141 CGCG
Bsu36I 517 CCTGAGG CC^T[ACGT]AGG
1087 CCTAAGG
DpnII 810 GATC ^GATC
1296 GATC
DsaI 10 CCGCGG C^C[GA][CT]GG
908 CCATGG
EaeI 702 TGGCCG [CT]^GGCC[GA]
1002 TGGCCA
Eco81I 517 CCTGAGG CC^T[ACGT]AGG
1087 CCTAAGG
FokI 374 GGATG GGATG(9/13)
659 GGATG
HinfI 951 GAGTC G^A[ACGT]TC
995 GAATC
MboI 810 GATC ^GATC
1296 GATC
MboII 1107 GAAGA GAAGA(8/7)
1152 GAAGA
MspA1I 10 CCGCGG C[AC]G^C[GT]G
1069 CAGCGG
MvnI 11 CGCG CG^CG
1141 CGCG
NaeI 637 GCCGGC GCC^GGC
1234 GCCGGC
NdeII 810 GATC ^GATC
1296 GATC
NgoMIV 637 GCCGGC G^CCGGC
1234 GCCGGC
PstI 175 CTGCAG CTGCA^G
414 CTGCAG
Sau3AI 810 GATC ^GATC
1296 GATC
SfaNI 634 GATGC GCATC(5/9)
71 GCATC
SmaI 48 CCCGGG CCC^GGG
610 CCCGGG
XmaCI 48 CCCGGG C^CCGGG
610 CCCGGG
XmaI 48 CCCGGG C^CCGGG
610 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BpmI BsaI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MnlI MspI MvaI MwoI NciI
NlaIII NlaIV PpuMI RsaI Sau96I
ScrFI SfcI StyI TseI Tsp45I
TspRI XcmI