Restriction map for
pF1KB8715
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AlwI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BclI BfrI BglII BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstYI BstZ17I ClaI CpoI
DpnII DraI DrdI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV FbaI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MboI
MfeI MflI MluI MroI MseI
MslI MunI MvnI NarI NdeI
NdeII NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcyI 595 GGCGTC G[GA]^CG[CT]C
AhdI 733 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 148 GTGCAC G^TGCAC
ApaI 12 GGGCCC GGGCC^C
ApaLI 148 GTGCAC G^TGCAC
AspEI 733 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 648 CCCGGG C^[CT]CG[GA]G
BanI 1060 GGTGCC G^G[CT][GA]CC
BbsI 751 GAAGAC GAAGAC(2/6)
BbvCI 275 CCTCAGC CCTCAGC(-5/-2)
BciVI 358 GGATAC GTATCC(6/5)
BfaI 407 CTAG C^TAG
BlpI 474 GCTCAGC GC^T[ACGT]AGC
BpmI 994 CTGGAG CTGGAG(16/14)
Bpu1102I 474 GCTCAGC GC^T[ACGT]AGC
BpuAI 751 GAAGAC GAAGAC(2/6)
BsaHI 595 GGCGTC G[GA]^CG[CT]C
BsaI 1099 GAGACC GGTCTC(1/5)
BsaWI 548 TCCGGT [AT]^CCGG[AT]
BsoBI 648 CCCGGG C^[CT]CG[GA]G
BstEII 1107 GGTGACC G^GT[ACGT]ACC
BstPI 1107 GGTGACC G^GT[ACGT]ACC
CelII 474 GCTCAGC GC^T[ACGT]AGC
DraIII 78 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 733 GACCTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1107 GGTGACC G^GT[ACGT]ACC
EcoT22I 1 ATGCAT ATGCA^T
FauI 616 GCGGG CCCGC(4/6)
FseI 96 GGCCGGCC GGCCGG^CC
HgaI 596 GCGTC GACGC(5/10)
Hin1I 595 GGCGTC G[GA]^CG[CT]C
MaeI 407 CTAG C^TAG
MboII 751 GAAGA GAAGA(8/7)
NsiI 1 ATGCAT ATGCA^T
PflMI 165 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfiI 13 GGCCCCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 648 CCCGGG CCC^GGG
TfiI 263 GATTC G^A[AT]TC
Van91I 165 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 648 CCCGGG C^CCGGG
XmaI 648 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 630 CCTGAGG CC^T[ACGT]AGG
777 CCTGAGG
AspHI 75 GAGCAC G[AT]GC[AT]^C
148 GTGCAC
BalI 21 TGGCCA TGG^CCA
377 TGGCCA
BanII 12 GGGCCC G[GA]GC[CT]^C
804 GGGCTC
BsiHKAI 75 GAGCAC G[AT]GC[AT]^C
148 GTGCAC
BsmAI 1099 GAGAC GTCTC(1/5)
970 GTCTC
BspMI 144 GCAGGT ACCTGC(4/8)
579 GCAGGT
Bsu36I 630 CCTGAGG CC^T[ACGT]AGG
777 CCTGAGG
CfoI 944 GCGC GCG^C
999 GCGC
EaeI 21 TGGCCA [CT]^GGCC[GA]
377 TGGCCA
Eco81I 630 CCTGAGG CC^T[ACGT]AGG
777 CCTGAGG
HhaI 944 GCGC GCG^C
999 GCGC
HinP1I 944 GCGC G^CGC
999 GCGC
HphI 1107 GGTGA GGTGA(8/7)
674 TCACC
MluNI 21 TGGCCA TGG^CCA
377 TGGCCA
MscI 21 TGGCCA TGG^CCA
377 TGGCCA
PleI 754 GACTC GAGTC(4/5)
855 GACTC
PpuMI 607 AGGACCT [GA]G^G[AT]CC[CT]
1143 GGGTCCT
SphI 3 GCATGC GCATG^C
270 GCATGC
XcmI 1043 CCACAGGCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1113 CCATGGAAAGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BcnI BglI BmyI BsaJI BseRI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI Cac8I Cfr10I Cfr13I DdeI
DraII DsaI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeIII MnlI MspA1I
MspI MvaI MwoI NaeI NciI
NcoI NgoMIV NlaIII NlaIV NspI
PstI PvuII Sau96I ScrFI SfaNI
SfcI StuI StyI TseI Tsp45I
TspRI