Restriction map for
pF1KB8717
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccIII AcsI AflIII
AgeI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AviII AvrII BalI BanI BbeI
BbrPI BbsI BclI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssSI Bst1107I BstBI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DrdI Eco47III EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp509I XbaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 632 GTCTAC GT^[AC][GT]AC
AclI 376 AACGTT AA^CGTT
AcyI 163 GGCGTC G[GA]^CG[CT]C
AflII 267 CTTAAG C^TTAAG
AhdI 391 GACTTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 391 GACTTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 91 GAGCAC G[AT]GC[AT]^C
BamHI 807 GGATCC G^GATCC
BanII 1402 GGGCTC G[GA]GC[CT]^C
BbvCI 1396 GCTGAGG CCTCAGC(-5/-2)
BcgI 122 GCATGTTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 267 CTTAAG C^TTAAG
BglII 210 AGATCT A^GATCT
BsaHI 163 GGCGTC G[GA]^CG[CT]C
BsaWI 172 TCCGGT [AT]^CCGG[AT]
BsgI 642 CTGCAC GTGCAG(16/14)
BsiEI 140 CGGCCG CG[GA][CT]^CG
BsiHKAI 91 GAGCAC G[AT]GC[AT]^C
BsmBI 916 CGTCTC CGTCTC(1/5)
BsmI 733 GCATTC GAATGC(1/-1)
BsrDI 1095 CATTGC GCAATG(2/0)
BssHII 118 GCGCGC G^CGCGC
BstEII 1164 GGTGACC G^GT[ACGT]ACC
BstPI 1164 GGTGACC G^GT[ACGT]ACC
DraIII 980 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1336 CCGCGG C^C[GA][CT]GG
EagI 140 CGGCCG C^GGCCG
Eam1105I 391 GACTTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 140 CGGCCG C^GGCCG
Eco52I 140 CGGCCG C^GGCCG
EcoNI 279 CCTCCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1164 GGTGACC G^GT[ACGT]ACC
EcoT22I 731 ATGCAT ATGCA^T
HaeII 133 GGCGCT [GA]GCGC^[CT]
Hin1I 163 GGCGTC G[GA]^CG[CT]C
KspI 1336 CCGCGG CCGC^GG
MseI 268 TTAA T^TAA
NsiI 731 ATGCAT ATGCA^T
NspI 122 GCATGT [GA]CATG^[CT]
PflMI 1107 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 376 AACGTT AA^CGTT
PstI 196 CTGCAG CTGCA^G
PvuII 828 CAGCTG CAG^CTG
SacII 1336 CCGCGG CCGC^GG
Tru9I 268 TTAA T^TAA
Van91I 1107 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwNI 190 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
1108 CAGAGACTG
AocI 811 CCTGAGG CC^T[ACGT]AGG
1347 CCTCAGG
Asp700I 373 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1362 GAACTTCTTC
BciVI 1009 GGATAC GTATCC(6/5)
176 GTATCC
BfaI 904 CTAG C^TAG
1429 CTAG
BmyI 91 GAGCAC G[AGT]GC[ACT]^C
1402 GGGCTC
BsaI 1315 GAGACC GGTCTC(1/5)
289 GGTCTC
BseRI 106 CTCCTC GAGGAG(10/8)
500 GAGGAG
Bsp1286I 91 GAGCAC G[AGT]GC[ACT]^C
1402 GGGCTC
BstAPI 189 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1019 GCAAGATTTGC
Bsu36I 811 CCTGAGG CC^T[ACGT]AGG
1347 CCTCAGG
EaeI 140 CGGCCG [CT]^GGCC[GA]
308 CGGCCA
EarI 748 GAAGAG CTCTTC(1/4)
1434 GAAGAG
Eco81I 811 CCTGAGG CC^T[ACGT]AGG
1347 CCTCAGG
EcoT14I 422 CCTTGG C^C[AT][AT]GG
872 CCTTGG
Ksp632I 748 GAAGAG CTCTTC(1/4)
1434 GAAGAG
MaeI 904 CTAG C^TAG
1429 CTAG
MslI 543 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1182 CACTCGAGTG
PaeR7I 666 CTCGAG C^TCGAG
1184 CTCGAG
PpuMI 200 AGGTCCT [GA]G^G[AT]CC[CT]
1216 AGGTCCC
StuI 780 AGGCCT AGG^CCT
1244 AGGCCT
StyI 422 CCTTGG C^C[AT][AT]GG
872 CCTTGG
TfiI 850 GATTC G^A[AT]TC
1087 GAATC
XhoI 666 CTCGAG C^TCGAG
1184 CTCGAG
XmnI 373 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1362 GAACTTCTTC
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspI AvaI AvaII BbvI BcnI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflFI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TseI
Tsp45I TspRI Tth111I TthHB8I XhoII