Restriction map for
pF1KB8718
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# NO CUT ENZYMES ( 101 enzymes) #
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Acc65I AclI AcsI AflII AgeI
AhdI AlwNI Aor51HI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbrPI BbsI BciVI
BclI BfrI BglI BglII BlnI
BpuAI BsaAI BsaI BsiWI BsmI
Bsp1407I BspDI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MfeI MluI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I XbaI
XcmI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 449 GTCTAC GT^[AC][GT]AC
Alw44I 672 GTGCAC G^TGCAC
ApaLI 672 GTGCAC G^TGCAC
Asp700I 936 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1594 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1810 GGATCC G^GATCC
BanII 34 GAGCTC G[GA]GC[CT]^C
BbeI 394 GGCGCC GGCGC^C
BbvCI 1711 GCTGAGG CCTCAGC(-5/-2)
BfaI 679 CTAG C^TAG
BlpI 711 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 711 GCTCAGC GC^T[ACGT]AGC
BsaBI 964 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1093 GAGACG CGTCTC(1/5)
BspHI 1139 TCATGA T^CATGA
BspMI 604 GCAGGT ACCTGC(4/8)
BsrDI 125 GCAATG GCAATG(2/0)
CelII 711 GCTCAGC GC^T[ACGT]AGC
DraIII 1066 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1236 GAAGAG CTCTTC(1/4)
EcoNI 1100 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1351 GTCAAC GT[CT]^[GA]AC
HindII 1351 GTCAAC GT[CT]^[GA]AC
KasI 394 GGCGCC G^GCGCC
Ksp632I 1236 GAAGAG CTCTTC(1/4)
MaeI 679 CTAG C^TAG
MamI 964 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1018 GCCGGC GCC^GGC
NarI 394 GGCGCC GG^CGCC
NgoMIV 1018 GCCGGC G^CCGGC
PaeR7I 232 CTCGAG C^TCGAG
PflFI 1594 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 906 GACTC GAGTC(4/5)
PshAI 1368 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 520 CTGCAG CTGCA^G
RcaI 1139 TCATGA T^CATGA
SacI 34 GAGCTC GAGCT^C
SapI 1236 GAAGAGC GCTCTTC(1/4)
SfoI 394 GGCGCC GGC^GCC
SmaI 110 CCCGGG CCC^GGG
TfiI 1330 GATTC G^A[AT]TC
Tth111I 1594 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 232 CTCGAG C^TCGAG
XmaCI 110 CCCGGG C^CCGGG
XmaI 110 CCCGGG C^CCGGG
XmnI 936 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 1261 GACGTC GACGT^C
1597 GACGTC
AccIII 950 TCCGGA T^CCGGA
1739 TCCGGA
BcgI 998 GCAACGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1241 GCAGCAAGGTCG
BseAI 950 TCCGGA T^CCGGA
1739 TCCGGA
BsiEI 657 CGACCG CG[GA][CT]^CG
699 CGACCG
BsmAI 631 GAGAC GTCTC(1/5)
1093 GAGAC
BspEI 950 TCCGGA T^CCGGA
1739 TCCGGA
BspLU11I 1496 ACATGT A^CATGT
1787 ACATGT
BsrFI 683 ACCGGC [GA]^CCGG[CT]
1018 GCCGGC
BstYI 1614 GGATCT [GA]^GATC[CT]
1810 GGATCC
Cfr10I 683 ACCGGC [GA]^CCGG[CT]
1018 GCCGGC
DrdI 658 GACCGGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1182 GACGGAGGTGTC
DsaI 366 CCGTGG C^C[GA][CT]GG
1670 CCGTGG
HaeII 158 AGCGCC [GA]GCGC^[CT]
394 GGCGCC
HgaI 954 GACGC GACGC(5/10)
1095 GACGC
HinfI 906 GACTC G^A[ACGT]TC
1330 GATTC
MaeIII 811 GTCAC ^GT[ACGT]AC
1405 GTCAC
MflI 1614 GGATCT [GA]^GATC[CT]
1810 GGATCC
MroI 950 TCCGGA T^CCGGA
1739 TCCGGA
PflMI 306 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
559 CCAAGTGCTGG
PpuMI 227 AGGACCT [GA]G^G[AT]CC[CT]
419 GGGACCT
SfcI 514 CTACAG C^T[GA][CT]AG
520 CTGCAG
SmlI 37 CTCAAG C^T[CT][GA]AG
232 CTCGAG
Tsp45I 811 GTCAC ^GT[GC]AC
1405 GTCAC
Van91I 306 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
559 CCAAGTGCTGG
XhoII 1614 GGATCT [GA]^GATC[CT]
1810 GGATCC
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AocI AspHI AvaI
AvaII BalI BanI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI MaeII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI TspRI TthHB8I