Restriction map for
pF1KB8719
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaWI BseAI BsgI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfuI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 663 GAATTC [GA]^AATT[CT]
AlwNI 344 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 663 GAATTC [GA]^AATT[CT]
AspHI 604 GAGCAC G[AT]GC[AT]^C
BbeI 1326 GGCGCC GGCGC^C
BbsI 448 GTCTTC GAAGAC(2/6)
BbvCI 550 GCTGAGG CCTCAGC(-5/-2)
BfaI 442 CTAG C^TAG
BpuAI 448 GTCTTC GAAGAC(2/6)
BsaAI 1003 TACGTG [CT]AC^GT[GA]
BsaBI 246 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 640 GAGACC GGTCTC(1/5)
BsiEI 381 CGGCCG CG[GA][CT]^CG
BsiHKAI 604 GAGCAC G[AT]GC[AT]^C
BsmBI 4 GAGACG CGTCTC(1/5)
BsrBI 1057 GAGCGG CCGCTC(-3/-3)
BsrFI 1323 ACCGGC [GA]^CCGG[CT]
BssSI 465 CACGAG CACGAG(-5/-1)
BstYI 1034 AGATCC [GA]^GATC[CT]
Cfr10I 1323 ACCGGC [GA]^CCGG[CT]
DsaI 378 CCGCGG C^C[GA][CT]GG
EagI 381 CGGCCG C^GGCCG
EclXI 381 CGGCCG C^GGCCG
Eco52I 381 CGGCCG C^GGCCG
EcoNI 111 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 663 GAATTC G^AATTC
HindIII 1412 AAGCTT A^AGCTT
HphI 1154 TCACC GGTGA(8/7)
KasI 1326 GGCGCC G^GCGCC
KspI 378 CCGCGG CCGC^GG
MaeI 442 CTAG C^TAG
MaeII 1004 ACGT A^CGT
MamI 246 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1034 AGATCC [GA]^GATC[CT]
NarI 1326 GGCGCC GG^CGCC
NspI 339 GCATGC [GA]CATG^[CT]
PaeR7I 878 CTCGAG C^TCGAG
PvuII 706 CAGCTG CAG^CTG
SacII 378 CCGCGG CCGC^GG
SfoI 1326 GGCGCC GGC^GCC
SgrAI 1322 CACCGGCG C[GA]^CCGG[CT]G
SmaI 284 CCCGGG CCC^GGG
SphI 339 GCATGC GCATG^C
TfiI 1140 GAATC G^A[AT]TC
Tsp509I 664 AATT ^AATT
XhoI 878 CTCGAG C^TCGAG
XhoII 1034 AGATCC [GA]^GATC[CT]
XmaCI 284 CCCGGG C^CCGGG
XmaI 284 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 6 GACGCC G[GA]^CG[CT]C
1326 GGCGCC
AlwI 861 GATCC GGATC(4/5)
1035 GATCC
AocI 331 CCTGAGG CC^T[ACGT]AGG
753 CCTTAGG
ApaI 840 GGGCCC GGGCC^C
1235 GGGCCC
BanI 785 GGTGCC G^G[CT][GA]CC
1326 GGCGCC
BcgI 1083 CGAGGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
16 GCATCAGCGTCG
BsaHI 6 GACGCC G[GA]^CG[CT]C
1326 GGCGCC
Bsu36I 331 CCTGAGG CC^T[ACGT]AGG
753 CCTTAGG
Eco81I 331 CCTGAGG CC^T[ACGT]AGG
753 CCTTAGG
EcoT14I 269 CCTTGG C^C[AT][AT]GG
509 CCAAGG
HaeII 1196 AGCGCC [GA]GCGC^[CT]
1326 GGCGCC
Hin1I 6 GACGCC G[GA]^CG[CT]C
1326 GGCGCC
MaeIII 307 GTGAC ^GT[ACGT]AC
1132 GTGAC
MseI 713 TTAA T^TAA
1031 TTAA
MslI 348 CACTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
498 CATGGAGGTG
PstI 1303 CTGCAG CTGCA^G
1390 CTGCAG
SfaNI 237 GATGC GCATC(5/9)
16 GCATC
SfiI 1077 GGCCAACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1236 GGCCCTGCGGGCC
StuI 387 AGGCCT AGG^CCT
1164 AGGCCT
StyI 269 CCTTGG C^C[AT][AT]GG
509 CCAAGG
Tru9I 713 TTAA T^TAA
1031 TTAA
Tsp45I 307 GTGAC ^GT[GC]AC
1132 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BalI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI TaqI TseI TspRI TthHB8I