Restriction map for
pF1KB8720
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsaI BsgI
BsiWI BsmI BspDI BspLU11I BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI KspI MamI
MluI NcoI NdeI NheI NruI
NsiI NspI NspV PacI PflFI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfuI SnaBI SpeI SphI SspI
StyI SwaI Tth111I XbaI XcmI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccIII 1212 TCCGGA T^CCGGA
AcyI 546 GGCGCC G[GA]^CG[CT]C
AflIII 1977 ACACGT A^C[GA][CT]GT
AgeI 2064 ACCGGT A^CCGGT
ApaI 466 GGGCCC GGGCC^C
Asp700I 19 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2051 TGGCCA TGG^CCA
BbeI 546 GGCGCC GGCGC^C
BbrPI 1978 CACGTG CAC^GTG
BbvCI 1184 CCTCAGC CCTCAGC(-5/-2)
BglI 827 GCCACTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1978 CACGTG [CT]AC^GT[GA]
BsaHI 546 GGCGCC G[GA]^CG[CT]C
BseAI 1212 TCCGGA T^CCGGA
Bsp1407I 245 TGTACA T^GTACA
BspEI 1212 TCCGGA T^CCGGA
BspHI 2018 TCATGA T^CATGA
BspMI 789 ACCTGC ACCTGC(4/8)
BsrDI 1660 GCAATG GCAATG(2/0)
BsrGI 245 TGTACA T^GTACA
BstAPI 958 GCATCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1990 GGATCT [GA]^GATC[CT]
DraI 1351 TTTAAA TTT^AAA
DraIII 1514 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1598 CCGTGG C^C[GA][CT]GG
EarI 1871 CTCTTC CTCTTC(1/4)
EcoRV 1882 GATATC GAT^ATC
HgaI 1475 GACGC GACGC(5/10)
Hin1I 546 GGCGCC G[GA]^CG[CT]C
HindIII 1402 AAGCTT A^AGCTT
KasI 546 GGCGCC G^GCGCC
Ksp632I 1871 CTCTTC CTCTTC(1/4)
MfeI 1267 CAATTG C^AATTG
MflI 1990 GGATCT [GA]^GATC[CT]
MluNI 2051 TGGCCA TGG^CCA
MroI 1212 TCCGGA T^CCGGA
MscI 2051 TGGCCA TGG^CCA
MslI 766 CACCGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1267 CAATTG C^AATTG
NarI 546 GGCGCC GG^CGCC
NotI 1419 GCGGCCGC GC^GGCCGC
PaeR7I 656 CTCGAG C^TCGAG
PflMI 336 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2064 ACCGGT A^CCGGT
PmaCI 1978 CACGTG CAC^GTG
PmlI 1978 CACGTG CAC^GTG
PpuMI 1071 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1100 CTGCAG CTGCA^G
RcaI 2018 TCATGA T^CATGA
SacI 2091 GAGCTC GAGCT^C
SapI 1870 GCTCTTC GCTCTTC(1/4)
SbfI 1099 CCTGCAGG CCTGCA^GG
ScaI 1807 AGTACT AGT^ACT
SfoI 546 GGCGCC GGC^GCC
SgrAI 2063 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 1099 CCTGCAGG CCTGCA^GG
SspBI 245 TGTACA T^GTACA
StuI 2002 AGGCCT AGG^CCT
Van91I 336 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 656 CTCGAG C^TCGAG
XhoII 1990 GGATCT [GA]^GATC[CT]
XmnI 19 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1409 CGCG CG^CG
1902 CGCG
AfaI 246 GTAC GT^AC
1808 GTAC
Alw44I 969 GTGCAC G^TGCAC
1431 GTGCAC
AocI 1756 CCTCAGG CC^T[ACGT]AGG
2116 CCTGAGG
ApaLI 969 GTGCAC G^TGCAC
1431 GTGCAC
BlpI 1506 GCTGAGC GC^T[ACGT]AGC
2109 GCTGAGC
Bpu1102I 1506 GCTGAGC GC^T[ACGT]AGC
2109 GCTGAGC
BsiEI 815 CGGCCG CG[GA][CT]^CG
1420 CGGCCG
BsmBI 419 CGTCTC CGTCTC(1/5)
1629 GAGACG
BsrBI 659 GAGCGG CCGCTC(-3/-3)
951 GAGCGG
BstUI 1409 CGCG CG^CG
1902 CGCG
BstXI 1592 CCAGGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1706 CCAGGCTGGTGG
Bsu36I 1756 CCTCAGG CC^T[ACGT]AGG
2116 CCTGAGG
CelII 1506 GCTGAGC GC^T[ACGT]AGC
2109 GCTGAGC
EagI 815 CGGCCG C^GGCCG
1420 CGGCCG
EclXI 815 CGGCCG C^GGCCG
1420 CGGCCG
Eco52I 815 CGGCCG C^GGCCG
1420 CGGCCG
Eco81I 1756 CCTCAGG CC^T[ACGT]AGG
2116 CCTGAGG
HaeII 546 GGCGCC [GA]GCGC^[CT]
1550 GGCGCT
MvnI 1409 CGCG CG^CG
1902 CGCG
NaeI 813 GCCGGC GCC^GGC
1547 GCCGGC
NgoMIV 813 GCCGGC G^CCGGC
1547 GCCGGC
PvuII 749 CAGCTG CAG^CTG
2107 CAGCTG
RsaI 246 GTAC GT^AC
1808 GTAC
SfcI 1100 CTGCAG C^T[GA][CT]AG
1985 CTGTAG
SmlI 656 CTCGAG C^T[CT][GA]AG
2152 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SmaI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmaCI XmaI