Restriction map for
pF1KB8721
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Aor51HI ApoI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BciVI BclI BfrI
BlnI BsaAI BsaHI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eam1105I
Eco47III EcoRI EcoRV EcoT22I FbaI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NarI NdeI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 618 CGCG CG^CG
AfaI 1100 GTAC GT^AC
AocI 228 CCTCAGG CC^T[ACGT]AGG
AseI 868 ATTAAT AT^TAAT
AsnI 868 ATTAAT AT^TAAT
BanI 712 GGTGCC G^G[CT][GA]CC
BbsI 1614 GAAGAC GAAGAC(2/6)
BbvCI 1522 GCTGAGG CCTCAGC(-5/-2)
BcgI 311 GCACAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1592 AGATCT A^GATCT
BpuAI 1614 GAAGAC GAAGAC(2/6)
BsaBI 960 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1160 GTGCAG GTGCAG(16/14)
BsiEI 162 CGGCCG CG[GA][CT]^CG
Bsp1407I 1099 TGTACA T^GTACA
BsrGI 1099 TGTACA T^GTACA
BssHII 617 GCGCGC G^CGCGC
BssSI 1540 CTCGTG CACGAG(-5/-1)
BstAPI 1357 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 180 GGTGACC G^GT[ACGT]ACC
BstPI 180 GGTGACC G^GT[ACGT]ACC
BstUI 618 CGCG CG^CG
Bsu36I 228 CCTCAGG CC^T[ACGT]AGG
DraIII 207 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4 GACAGCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 162 CGGCCG C^GGCCG
EarI 79 GAAGAG CTCTTC(1/4)
EclXI 162 CGGCCG C^GGCCG
Eco52I 162 CGGCCG C^GGCCG
Eco81I 228 CCTCAGG CC^T[ACGT]AGG
EcoNI 975 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 180 GGTGACC G^GT[ACGT]ACC
FauI 387 CCCGC CCCGC(4/6)
FseI 372 GGCCGGCC GGCCGG^CC
HincII 895 GTCGAC GT[CT]^[GA]AC
HindII 895 GTCGAC GT[CT]^[GA]AC
Ksp632I 79 GAAGAG CTCTTC(1/4)
MaeII 1385 ACGT A^CGT
MamI 960 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 618 CGCG CG^CG
NcoI 527 CCATGG C^CATGG
NheI 852 GCTAGC G^CTAGC
PpuMI 425 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 868 ATTAAT AT^TAAT
RsaI 1100 GTAC GT^AC
SalI 895 GTCGAC G^TCGAC
SspBI 1099 TGTACA T^GTACA
StuI 401 AGGCCT AGG^CCT
XcmI 452 CCATCCAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 895 GTCGAC GT^[AC][GT]AC
1174 GTCTAC
Alw44I 204 GTGCAC G^TGCAC
1408 GTGCAC
AlwI 478 GATCC GGATC(4/5)
1213 GATCC
ApaLI 204 GTGCAC G^TGCAC
1408 GTGCAC
BlpI 99 GCTGAGC GC^T[ACGT]AGC
1370 GCTTAGC
Bpu1102I 99 GCTGAGC GC^T[ACGT]AGC
1370 GCTTAGC
BsaI 904 GAGACC GGTCTC(1/5)
953 GGTCTC
BsmI 1362 GAATGC GAATGC(1/-1)
1309 GCATTC
BspMI 95 ACCTGC ACCTGC(4/8)
1110 ACCTGC
BsrFI 164 GCCGGC [GA]^CCGG[CT]
373 GCCGGC
CelII 99 GCTGAGC GC^T[ACGT]AGC
1370 GCTTAGC
CfoI 617 GCGC GCG^C
619 GCGC
Cfr10I 164 GCCGGC [GA]^CCGG[CT]
373 GCCGGC
HhaI 617 GCGC GCG^C
619 GCGC
HinP1I 617 GCGC G^CGC
619 GCGC
HphI 180 GGTGA GGTGA(8/7)
939 GGTGA
MaeIII 181 GTGAC ^GT[ACGT]AC
1543 GTGAC
MseI 706 TTAA T^TAA
869 TTAA
NaeI 164 GCCGGC GCC^GGC
373 GCCGGC
NgoMIV 164 GCCGGC G^CCGGC
373 GCCGGC
PleI 1606 GACTC GAGTC(4/5)
72 GAGTC
PvuII 361 CAGCTG CAG^CTG
857 CAGCTG
SfaNI 1189 GATGC GCATC(5/9)
932 GCATC
SfiI 154 GGCCCCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1295 GGCCCAGTGGGCC
SmaI 625 CCCGGG CCC^GGG
657 CCCGGG
TaqI 896 TCGA T^CGA
1512 TCGA
Tru9I 706 TTAA T^TAA
869 TTAA
Tsp45I 181 GTGAC ^GT[GC]AC
1543 GTGAC
TthHB8I 896 TCGA T^CGA
1512 TCGA
XmaCI 625 CCCGGG C^CCGGG
657 CCCGGG
XmaI 625 CCCGGG C^CCGGG
657 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AluI AlwNI ApaI AspHI
AvaI AvaII BalI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeI MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PstI
Sau3AI Sau96I ScrFI SfcI StyI
TfiI TseI Tsp509I TspRI Van91I
XhoII