Restriction map for
pF1KB8723
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AviII AvrII BamHI BbeI
BbsI BbvCI BcgI BfaI BglI
BglII BlnI BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KspI MaeI MamI MfeI
MflI MluI MroI MunI MvnI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI SwaI TaqI TfiI
TthHB8I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1357 GGTACC G^GTACC
AflII 1230 CTTAAG C^TTAAG
AseI 778 ATTAAT AT^TAAT
AsnI 778 ATTAAT AT^TAAT
Asp718I 1357 GGTACC G^GTACC
AspI 1144 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1096 CCCGAG C^[CT]CG[GA]G
AvaII 1143 GGACC G^G[AT]CC
BalI 119 TGGCCA TGG^CCA
BciVI 1320 GTATCC GTATCC(6/5)
BclI 1176 TGATCA T^GATCA
BfrI 1230 CTTAAG C^TTAAG
BsaI 283 GAGACC GGTCTC(1/5)
BsoBI 1096 CCCGAG C^[CT]CG[GA]G
BspMI 235 ACCTGC ACCTGC(4/8)
BsrFI 995 GCCGGC [GA]^CCGG[CT]
Cfr10I 995 GCCGGC [GA]^CCGG[CT]
DrdI 399 GACTGTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 119 TGGCCA [CT]^GGCC[GA]
EcoRI 1245 GAATTC G^AATTC
EcoRV 664 GATATC GAT^ATC
FbaI 1176 TGATCA T^GATCA
HgaI 1040 GCGTC GACGC(5/10)
HindIII 1233 AAGCTT A^AGCTT
KpnI 1357 GGTACC GGTAC^C
MaeIII 718 GTGAC ^GT[ACGT]AC
MluNI 119 TGGCCA TGG^CCA
MscI 119 TGGCCA TGG^CCA
MspA1I 691 CAGCTG C[AC]G^C[GT]G
NaeI 995 GCCGGC GCC^GGC
NcoI 1353 CCATGG C^CATGG
NgoMIV 995 GCCGGC G^CCGGC
NspI 1389 GCATGC [GA]CATG^[CT]
PflFI 1144 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1142 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 778 ATTAAT AT^TAAT
PvuII 691 CAGCTG CAG^CTG
SacI 151 GAGCTC GAGCT^C
SbfI 891 CCTGCAGG CCTGCA^GG
SexAI 1145 ACCTGGT A^CC[AT]GGT
SphI 1389 GCATGC GCATG^C
Sse8387I 891 CCTGCAGG CCTGCA^GG
StuI 221 AGGCCT AGG^CCT
Tsp45I 718 GTGAC ^GT[GC]AC
Tth111I 1144 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 122 CCATTGCCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 535 GTAGAC GT^[AC][GT]AC
788 GTAGAC
AcsI 751 AAATTT [GA]^AATT[CT]
1245 GAATTC
ApoI 751 AAATTT [GA]^AATT[CT]
1245 GAATTC
BanI 556 GGTGCC G^G[CT][GA]CC
1357 GGTACC
BbrPI 955 CACGTG CAC^GTG
1312 CACGTG
BcnI 831 CCCGG CC^[GC]GG
1491 CCCGG
BlpI 454 GCTCAGC GC^T[ACGT]AGC
1296 GCTGAGC
Bpu1102I 454 GCTCAGC GC^T[ACGT]AGC
1296 GCTGAGC
BsaAI 955 CACGTG [CT]AC^GT[GA]
1312 CACGTG
BseRI 793 CTCCTC GAGGAG(10/8)
796 CTCCTC
BsgI 217 GTGCAG GTGCAG(16/14)
688 GTGCAG
BstAPI 93 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
885 GCAGTGCCTGC
CelII 454 GCTCAGC GC^T[ACGT]AGC
1296 GCTGAGC
DraII 113 AGGCCCT [GA]G^G[ACGT]CC[CT]
1142 AGGACCT
DsaI 67 CCACGG C^C[GA][CT]GG
1353 CCATGG
EcoNI 99 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
321 CCTTGCCCAGG
EcoO109I 113 AGGCCCT [GA]G^G[ACGT]CC[CT]
1142 AGGACCT
EcoT22I 812 ATGCAT ATGCA^T
1018 ATGCAT
FauI 159 CCCGC CCCGC(4/6)
18 GCGGG
MaeII 956 ACGT A^CGT
1313 ACGT
MseI 779 TTAA T^TAA
1231 TTAA
NciI 831 CCCGG CC^[GC]GG
1491 CCCGG
NsiI 812 ATGCAT ATGCA^T
1018 ATGCAT
PflMI 341 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1140 CCAGGACCTGG
PmaCI 955 CACGTG CAC^GTG
1312 CACGTG
PmlI 955 CACGTG CAC^GTG
1312 CACGTG
SapI 841 GAAGAGC GCTCTTC(1/4)
153 GCTCTTC
SmlI 547 CTCAAG C^T[CT][GA]AG
1230 CTTAAG
Tru9I 779 TTAA T^TAA
1231 TTAA
Van91I 341 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1140 CCAGGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI BanII BbvI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MboI MboII
MnlI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp509I TspRI