Restriction map for
pF1KB8724
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Aor51HI
ApaI AscI AseI AsnI Asp718I
AviII AvrII BanII BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 729 GTAC GT^AC
AhdI 373 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 613 GTGCAC G^TGCAC
AocI 581 CCTTAGG CC^T[ACGT]AGG
ApaLI 613 GTGCAC G^TGCAC
AspEI 373 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 613 GTGCAC G[AT]GC[AT]^C
BalI 1562 TGGCCA TGG^CCA
BanI 1429 GGCACC G^G[CT][GA]CC
BbvCI 820 GCTGAGG CCTCAGC(-5/-2)
BmyI 613 GTGCAC G[AGT]GC[ACT]^C
BsaAI 1129 TACGTG [CT]AC^GT[GA]
BsaI 598 GAGACC GGTCTC(1/5)
BseRI 304 GAGGAG GAGGAG(10/8)
BsiEI 1497 CGACCG CG[GA][CT]^CG
BsiHKAI 613 GTGCAC G[AT]GC[AT]^C
BsmAI 598 GAGAC GTCTC(1/5)
Bsp1286I 613 GTGCAC G[AGT]GC[ACT]^C
BspHI 881 TCATGA T^CATGA
BspMI 1574 GCAGGT ACCTGC(4/8)
BstAPI 1532 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 581 CCTTAGG CC^T[ACGT]AGG
CfoI 1195 GCGC GCG^C
DraI 631 TTTAAA TTT^AAA
DraII 896 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 1451 CCACGG C^C[GA][CT]GG
EaeI 1562 TGGCCA [CT]^GGCC[GA]
Eam1105I 373 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 581 CCTTAGG CC^T[ACGT]AGG
EcoO109I 896 AGGGCCT [GA]G^G[ACGT]CC[CT]
HgaI 456 GCGTC GACGC(5/10)
HhaI 1195 GCGC GCG^C
HinP1I 1195 GCGC G^CGC
MaeII 1130 ACGT A^CGT
MluNI 1562 TGGCCA TGG^CCA
MscI 1562 TGGCCA TGG^CCA
PaeR7I 1250 CTCGAG C^TCGAG
RcaI 881 TCATGA T^CATGA
RsaI 729 GTAC GT^AC
SapI 1545 GCTCTTC GCTCTTC(1/4)
SexAI 512 ACCAGGT A^CC[AT]GGT
SmaI 1208 CCCGGG CCC^GGG
SmlI 1250 CTCGAG C^T[CT][GA]AG
XcmI 426 CCAGCTTTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1250 CTCGAG C^TCGAG
XmaCI 1208 CCCGGG C^CCGGG
XmaI 1208 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 370 GTCGAC GT^[AC][GT]AC
1495 GTCGAC
AspI 364 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
511 GACCAGGTC
AvaI 1208 CCCGGG C^[CT]CG[GA]G
1250 CTCGAG
BamHI 449 GGATCC G^GATCC
973 GGATCC
BbsI 346 GAAGAC GAAGAC(2/6)
1182 GAAGAC
BcnI 1208 CCCGG CC^[GC]GG
1209 CCGGG
BfaI 166 CTAG C^TAG
1297 CTAG
BpuAI 346 GAAGAC GAAGAC(2/6)
1182 GAAGAC
BsaBI 1317 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1377 GATCGCCATC
BsmFI 1159 GTCCC GGGAC(10/14)
1305 GTCCC
BsoBI 1208 CCCGGG C^[CT]CG[GA]G
1250 CTCGAG
FauI 153 CCCGC CCCGC(4/6)
15 GCGGG
MaeI 166 CTAG C^TAG
1297 CTAG
MaeIII 277 GTCAC ^GT[ACGT]AC
1447 GTGAC
MamI 1317 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1377 GATCGCCATC
NciI 1208 CCCGG CC^[GC]GG
1209 CCGGG
NspI 236 GCATGC [GA]CATG^[CT]
1145 GCATGC
PflFI 364 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
511 GACCAGGTC
PflMI 281 CCAACGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1095 CCAGGAGCTGG
PstI 10 CTGCAG CTGCA^G
194 CTGCAG
PvuII 1120 CAGCTG CAG^CTG
1536 CAGCTG
SalI 370 GTCGAC G^TCGAC
1495 GTCGAC
SfcI 10 CTGCAG C^T[GA][CT]AG
194 CTGCAG
SphI 236 GCATGC GCATG^C
1145 GCATGC
TfiI 291 GATTC G^A[AT]TC
948 GATTC
Tsp45I 277 GTCAC ^GT[GC]AC
1447 GTGAC
Tth111I 364 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
511 GACCAGGTC
Van91I 281 CCAACGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1095 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI Asp700I AvaII BbvI BpmI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI