Restriction map for
pF1KB8725
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII AclI AcsI AflII AflIII
AgeI AhdI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AspI
BbeI BbvCI BciVI BfrI BglI
BlpI Bpu1102I BsiEI BsiWI BsmBI
BspDI BspLU11I BspMI BsrBI BsrFI
BssHII Bst1107I BstBI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoRI FauI FseI HincII HindII
HindIII HpaI KasI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1066 GGTACC G^GTACC
AccI 427 GTCTAC GT^[AC][GT]AC
AccII 33 CGCG CG^CG
AccIII 1653 TCCGGA T^CCGGA
AcyI 688 GACGCC G[GA]^CG[CT]C
AlwNI 1542 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 896 AGCGCT AGC^GCT
Asp718I 1066 GGTACC G^GTACC
AvaI 489 CTCGGG C^[CT]CG[GA]G
AviII 1688 TGCGCA TGC^GCA
AvrII 1470 CCTAGG C^CTAGG
BalI 243 TGGCCA TGG^CCA
BamHI 419 GGATCC G^GATCC
BbrPI 925 CACGTG CAC^GTG
BbsI 1408 GTCTTC GAAGAC(2/6)
BcgI 1304 GCATCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 470 AGATCT A^GATCT
BlnI 1470 CCTAGG C^CTAGG
BpmI 15 CTGGAG CTGGAG(16/14)
BpuAI 1408 GTCTTC GAAGAC(2/6)
BsaHI 688 GACGCC G[GA]^CG[CT]C
BsaWI 1653 TCCGGA [AT]^CCGG[AT]
BseAI 1653 TCCGGA T^CCGGA
BsoBI 489 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1013 TGTACA T^GTACA
BspEI 1653 TCCGGA T^CCGGA
BsrGI 1013 TGTACA T^GTACA
BssSI 58 CACGAG CACGAG(-5/-1)
BstUI 33 CGCG CG^CG
BstXI 1379 CCAGTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 896 AGCGCT AGC^GCT
EcoRV 82 GATATC GAT^ATC
EcoT22I 1979 ATGCAT ATGCA^T
FspI 1688 TGCGCA TGC^GCA
HgaI 688 GACGC GACGC(5/10)
Hin1I 688 GACGCC G[GA]^CG[CT]C
KpnI 1066 GGTACC GGTAC^C
KspI 32 CCGCGG CCGC^GG
MluNI 243 TGGCCA TGG^CCA
MroI 1653 TCCGGA T^CCGGA
MscI 243 TGGCCA TGG^CCA
MvnI 33 CGCG CG^CG
NsiI 1979 ATGCAT ATGCA^T
PmaCI 925 CACGTG CAC^GTG
PmlI 925 CACGTG CAC^GTG
SacII 32 CCGCGG CCGC^GG
SspBI 1013 TGTACA T^GTACA
SspI 1058 AATATT AAT^ATT
TaqI 1358 TCGA T^CGA
TthHB8I 1358 TCGA T^CGA
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# TWO CUT ENZYMES ( 29 enzymes) #
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AvaII 1236 GGTCC G^G[AT]CC
1390 GGACC
BanI 1066 GGTACC G^G[CT][GA]CC
1320 GGTGCC
BclI 626 TGATCA T^GATCA
998 TGATCA
BsaAI 925 CACGTG [CT]AC^GT[GA]
1493 CACGTA
BsgI 892 GTGCAG GTGCAG(16/14)
1330 GTGCAG
BsmI 309 GAATGC GAATGC(1/-1)
1007 GCATTC
BspHI 1169 TCATGA T^CATGA
1265 TCATGA
BsrDI 1398 CATTGC GCAATG(2/0)
71 GCAATG
BstAPI 556 GCAATCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1981 GCATATTTTGC
BstEII 36 GGTCACC G^GT[ACGT]ACC
1956 GGTGACC
BstPI 36 GGTCACC G^GT[ACGT]ACC
1956 GGTGACC
DraII 584 GGGGCCT [GA]G^G[ACGT]CC[CT]
1746 AGGCCCT
EcoO109I 584 GGGGCCT [GA]G^G[ACGT]CC[CT]
1746 AGGCCCT
EcoO65I 36 GGTCACC G^GT[ACGT]ACC
1956 GGTGACC
FbaI 626 TGATCA T^GATCA
998 TGATCA
HaeII 896 AGCGCT [GA]GCGC^[CT]
1198 AGCGCC
MseI 362 TTAA T^TAA
646 TTAA
MspA1I 32 CCGCGG C[AC]G^C[GT]G
415 CAGCGG
NcoI 823 CCATGG C^CATGG
1217 CCATGG
PflMI 246 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
823 CCATGGCCTGG
PleI 781 GACTC GAGTC(4/5)
1436 GAGTC
RcaI 1169 TCATGA T^CATGA
1265 TCATGA
StuI 519 AGGCCT AGG^CCT
1137 AGGCCT
TfiI 525 GAATC G^A[AT]TC
1799 GAATC
Tru9I 362 TTAA T^TAA
646 TTAA
Tsp509I 576 AATT ^AATT
1026 AATT
TspRI 561 CACTG CA[GC]TG[ACGT][ACGT]^
1928 CAGTG
Van91I 246 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
823 CCATGGCCTGG
XcmI 971 CCATGTTCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1447 CCAGGAGCCTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AocI ApaLI AspHI BanII BbvI
BcnI BfaI BmyI BsaBI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraIII
DsaI EaeI EarI Eco81I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI SacI
Sau3AI Sau96I ScrFI SfaNI StyI
TseI Tsp45I XhoII